Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagH and yagI are separated by 4 nucleotides yagI and argF are separated by 138 nucleotides
b0271: yagH - CP4-6 prophage; predicted xylosidase/arabinosidase (NCBI), at 286,013 to 287,623
yagH
b0272: yagI - CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 287,628 to 288,386
yagI
b0273: argF - CP4-6 prophage; ornithine carbamoyltransferase 2, chain F (NCBI), at 288,525 to 289,529
argF
Position (kb) 287
288
289 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 286.655 kb on - strand, within yagH at 286.692 kb on + strand, within yagH at 286.692 kb on + strand, within yagH at 286.739 kb on + strand, within yagH at 286.789 kb on + strand, within yagH at 287.037 kb on - strand, within yagH at 287.070 kb on - strand, within yagH at 287.150 kb on - strand, within yagH at 287.230 kb on - strand, within yagH at 287.249 kb on + strand, within yagH at 287.253 kb on - strand, within yagH at 287.287 kb on + strand, within yagH at 287.293 kb on - strand, within yagH at 287.293 kb on - strand, within yagH at 287.302 kb on + strand, within yagH at 287.302 kb on + strand, within yagH at 287.302 kb on + strand, within yagH at 287.302 kb on + strand, within yagH at 287.310 kb on - strand, within yagH at 287.373 kb on - strand, within yagH at 287.373 kb on - strand, within yagH at 287.459 kb on + strand, within yagH at 287.489 kb on + strand at 287.489 kb on + strand at 287.496 kb on - strand at 287.599 kb on - strand at 287.604 kb on + strand at 287.643 kb on - strand at 287.643 kb on - strand at 287.670 kb on - strand at 287.743 kb on - strand, within yagI at 287.766 kb on + strand, within yagI at 287.782 kb on + strand, within yagI at 287.870 kb on + strand, within yagI at 287.904 kb on + strand, within yagI at 287.914 kb on + strand, within yagI at 287.914 kb on + strand, within yagI at 287.955 kb on + strand, within yagI at 288.049 kb on + strand, within yagI at 288.049 kb on + strand, within yagI at 288.056 kb on - strand, within yagI at 288.056 kb on - strand, within yagI at 288.057 kb on - strand, within yagI at 288.057 kb on - strand, within yagI at 288.103 kb on + strand, within yagI at 288.271 kb on + strand, within yagI at 288.271 kb on + strand, within yagI at 288.274 kb on + strand, within yagI at 288.282 kb on - strand, within yagI at 288.282 kb on - strand, within yagI at 288.308 kb on - strand, within yagI at 288.339 kb on - strand at 288.470 kb on - strand at 288.470 kb on - strand at 288.476 kb on + strand at 288.480 kb on - strand at 288.501 kb on - strand at 288.501 kb on - strand at 288.536 kb on - strand at 288.592 kb on - strand at 288.617 kb on - strand at 288.750 kb on + strand, within argF at 288.750 kb on + strand, within argF at 288.758 kb on - strand, within argF at 288.809 kb on - strand, within argF at 288.820 kb on + strand, within argF at 288.820 kb on + strand, within argF at 289.058 kb on - strand, within argF at 289.099 kb on + strand, within argF at 289.099 kb on - strand, within argF at 289.107 kb on - strand, within argF at 289.107 kb on - strand, within argF at 289.193 kb on + strand, within argF at 289.193 kb on + strand, within argF at 289.207 kb on + strand, within argF at 289.207 kb on + strand, within argF
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 286,655 - yagH b0271 0.40 +0.3 286,692 + yagH b0271 0.42 +0.4 286,692 + yagH b0271 0.42 -0.7 286,739 + yagH b0271 0.45 -0.8 286,789 + yagH b0271 0.48 +0.1 287,037 - yagH b0271 0.64 -0.5 287,070 - yagH b0271 0.66 -0.1 287,150 - yagH b0271 0.71 +0.3 287,230 - yagH b0271 0.76 +0.7 287,249 + yagH b0271 0.77 +0.2 287,253 - yagH b0271 0.77 -1.0 287,287 + yagH b0271 0.79 -0.3 287,293 - yagH b0271 0.79 -0.8 287,293 - yagH b0271 0.79 +0.7 287,302 + yagH b0271 0.80 +0.1 287,302 + yagH b0271 0.80 +0.7 287,302 + yagH b0271 0.80 -5.2 287,302 + yagH b0271 0.80 -1.3 287,310 - yagH b0271 0.81 +1.1 287,373 - yagH b0271 0.84 +1.6 287,373 - yagH b0271 0.84 +1.5 287,459 + yagH b0271 0.90 -0.3 287,489 + +0.2 287,489 + +0.5 287,496 - +0.5 287,599 - -0.3 287,604 + -0.3 287,643 - -1.5 287,643 - +0.2 287,670 - -1.2 287,743 - yagI b0272 0.15 +0.4 287,766 + yagI b0272 0.18 +1.1 287,782 + yagI b0272 0.20 -0.6 287,870 + yagI b0272 0.32 -0.0 287,904 + yagI b0272 0.36 +0.1 287,914 + yagI b0272 0.38 -1.5 287,914 + yagI b0272 0.38 +0.5 287,955 + yagI b0272 0.43 +0.2 288,049 + yagI b0272 0.55 -0.6 288,049 + yagI b0272 0.55 -0.8 288,056 - yagI b0272 0.56 -1.3 288,056 - yagI b0272 0.56 -2.6 288,057 - yagI b0272 0.57 +2.2 288,057 - yagI b0272 0.57 +0.0 288,103 + yagI b0272 0.63 -0.6 288,271 + yagI b0272 0.85 +0.8 288,271 + yagI b0272 0.85 -3.2 288,274 + yagI b0272 0.85 +0.5 288,282 - yagI b0272 0.86 +0.7 288,282 - yagI b0272 0.86 +0.7 288,308 - yagI b0272 0.90 -1.0 288,339 - +1.1 288,470 - -3.1 288,470 - -4.1 288,476 + -1.7 288,480 - +0.5 288,501 - -0.6 288,501 - +0.1 288,536 - -4.0 288,592 - -0.2 288,617 - +0.1 288,750 + argF b0273 0.22 +0.3 288,750 + argF b0273 0.22 -1.7 288,758 - argF b0273 0.23 -0.2 288,809 - argF b0273 0.28 +1.1 288,820 + argF b0273 0.29 -0.6 288,820 + argF b0273 0.29 -0.7 289,058 - argF b0273 0.53 +0.5 289,099 + argF b0273 0.57 -0.3 289,099 - argF b0273 0.57 +1.1 289,107 - argF b0273 0.58 +1.2 289,107 - argF b0273 0.58 -3.5 289,193 + argF b0273 0.66 -0.1 289,193 + argF b0273 0.66 +0.1 289,207 + argF b0273 0.68 +0.6 289,207 + argF b0273 0.68 +0.7
Or see this region's nucleotide sequence