Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeB and rcsF overlap by 4 nucleotides rcsF and metQ are separated by 117 nucleotides metQ and metI are separated by 39 nucleotides metI and metN overlap by 8 nucleotides
b0195: yaeB - hypothetical protein (NCBI), at 218,887 to 219,594
yaeB
b0196: rcsF - predicted outer membrane protein, signal (NCBI), at 219,591 to 219,995
rcsF
b0197: metQ - DL-methionine transporter subunit (NCBI), at 220,113 to 220,928
metQ
b0198: metI - DL-methionine transporter subunit (NCBI), at 220,968 to 221,621
metI
b0199: metN - DL-methionine transporter subunit (NCBI), at 221,614 to 222,645
metN
Position (kb) 220
221 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 219.200 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.261 kb on + strand, within yaeB at 219.312 kb on + strand, within yaeB at 219.446 kb on - strand, within yaeB at 219.465 kb on + strand, within yaeB at 219.466 kb on + strand, within yaeB at 219.489 kb on - strand, within yaeB at 219.513 kb on + strand, within yaeB at 219.548 kb on - strand at 219.598 kb on + strand at 219.598 kb on + strand at 219.643 kb on - strand, within rcsF at 219.678 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.756 kb on + strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.842 kb on - strand, within rcsF at 219.902 kb on + strand, within rcsF at 219.944 kb on - strand, within rcsF at 219.968 kb on + strand at 219.976 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.127 kb on - strand at 220.127 kb on - strand at 220.249 kb on - strand, within metQ at 220.321 kb on + strand, within metQ at 220.321 kb on + strand, within metQ at 220.323 kb on + strand, within metQ at 220.361 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.405 kb on + strand, within metQ at 220.405 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.504 kb on + strand, within metQ at 220.532 kb on + strand, within metQ at 220.543 kb on + strand, within metQ at 220.661 kb on + strand, within metQ at 220.675 kb on + strand, within metQ at 220.694 kb on - strand, within metQ at 220.694 kb on - strand, within metQ at 220.750 kb on - strand, within metQ at 220.754 kb on + strand, within metQ at 220.764 kb on + strand, within metQ at 220.825 kb on + strand, within metQ at 220.865 kb on + strand at 221.043 kb on - strand, within metI at 221.047 kb on + strand, within metI at 221.052 kb on + strand, within metI at 221.060 kb on - strand, within metI at 221.076 kb on + strand, within metI at 221.076 kb on + strand, within metI at 221.080 kb on + strand, within metI at 221.080 kb on + strand, within metI at 221.096 kb on - strand, within metI at 221.105 kb on - strand, within metI at 221.106 kb on - strand, within metI at 221.128 kb on - strand, within metI at 221.148 kb on + strand, within metI at 221.148 kb on + strand, within metI at 221.181 kb on + strand, within metI at 221.214 kb on + strand, within metI at 221.238 kb on + strand, within metI at 221.238 kb on + strand, within metI at 221.250 kb on - strand, within metI at 221.269 kb on - strand, within metI at 221.280 kb on + strand, within metI at 221.300 kb on - strand, within metI at 221.347 kb on - strand, within metI at 221.383 kb on - strand, within metI at 221.383 kb on - strand, within metI at 221.466 kb on - strand, within metI at 221.469 kb on - strand, within metI at 221.511 kb on - strand, within metI at 221.511 kb on - strand, within metI at 221.595 kb on - strand at 221.595 kb on - strand at 221.619 kb on + strand at 221.623 kb on + strand at 221.655 kb on - strand at 221.680 kb on + strand at 221.685 kb on + strand at 221.688 kb on - strand at 221.802 kb on + strand, within metN at 221.830 kb on + strand, within metN at 221.830 kb on + strand, within metN at 221.846 kb on + strand, within metN at 221.847 kb on + strand, within metN at 221.848 kb on + strand, within metN at 221.856 kb on + strand, within metN at 221.911 kb on - strand, within metN
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 219,200 + yaeB b0195 0.44 -1.2 219,240 + yaeB b0195 0.50 +0.8 219,240 + yaeB b0195 0.50 +1.1 219,261 + yaeB b0195 0.53 +1.0 219,312 + yaeB b0195 0.60 -2.6 219,446 - yaeB b0195 0.79 -0.5 219,465 + yaeB b0195 0.82 +1.1 219,466 + yaeB b0195 0.82 +0.8 219,489 - yaeB b0195 0.85 +0.7 219,513 + yaeB b0195 0.88 -0.2 219,548 - +0.6 219,598 + +1.6 219,598 + +0.7 219,643 - rcsF b0196 0.13 -2.4 219,678 + rcsF b0196 0.21 -1.1 219,680 + rcsF b0196 0.22 +0.1 219,680 + rcsF b0196 0.22 -0.2 219,680 + rcsF b0196 0.22 -0.7 219,720 - rcsF b0196 0.32 -0.0 219,720 - rcsF b0196 0.32 -0.5 219,756 + rcsF b0196 0.41 +0.6 219,764 - rcsF b0196 0.43 +0.7 219,764 - rcsF b0196 0.43 +0.3 219,764 - rcsF b0196 0.43 -0.1 219,842 - rcsF b0196 0.62 +0.3 219,902 + rcsF b0196 0.77 -2.1 219,944 - rcsF b0196 0.87 -0.5 219,968 + -3.0 219,976 - -0.2 220,041 - +0.6 220,041 - +1.9 220,041 - +1.4 220,127 - +0.3 220,127 - -0.9 220,249 - metQ b0197 0.17 -3.1 220,321 + metQ b0197 0.25 +1.0 220,321 + metQ b0197 0.25 +0.6 220,323 + metQ b0197 0.26 -0.1 220,361 - metQ b0197 0.30 +0.1 220,386 - metQ b0197 0.33 +1.3 220,386 - metQ b0197 0.33 -0.1 220,405 + metQ b0197 0.36 -1.6 220,405 + metQ b0197 0.36 -2.8 220,432 + metQ b0197 0.39 -1.0 220,432 + metQ b0197 0.39 +0.5 220,504 + metQ b0197 0.48 -1.9 220,532 + metQ b0197 0.51 +0.1 220,543 + metQ b0197 0.53 -0.0 220,661 + metQ b0197 0.67 +0.0 220,675 + metQ b0197 0.69 -2.0 220,694 - metQ b0197 0.71 -3.2 220,694 - metQ b0197 0.71 -0.6 220,750 - metQ b0197 0.78 -0.1 220,754 + metQ b0197 0.79 +0.4 220,764 + metQ b0197 0.80 +0.9 220,825 + metQ b0197 0.87 -1.5 220,865 + +1.0 221,043 - metI b0198 0.11 -0.4 221,047 + metI b0198 0.12 -1.4 221,052 + metI b0198 0.13 +0.5 221,060 - metI b0198 0.14 +0.0 221,076 + metI b0198 0.17 +0.3 221,076 + metI b0198 0.17 -0.6 221,080 + metI b0198 0.17 -0.4 221,080 + metI b0198 0.17 -2.6 221,096 - metI b0198 0.20 +0.5 221,105 - metI b0198 0.21 -0.7 221,106 - metI b0198 0.21 -2.2 221,128 - metI b0198 0.24 +1.0 221,148 + metI b0198 0.28 -2.8 221,148 + metI b0198 0.28 -0.0 221,181 + metI b0198 0.33 +0.0 221,214 + metI b0198 0.38 +1.8 221,238 + metI b0198 0.41 -1.5 221,238 + metI b0198 0.41 -0.4 221,250 - metI b0198 0.43 -0.5 221,269 - metI b0198 0.46 -1.1 221,280 + metI b0198 0.48 -1.1 221,300 - metI b0198 0.51 -0.3 221,347 - metI b0198 0.58 -1.8 221,383 - metI b0198 0.63 -0.7 221,383 - metI b0198 0.63 -0.1 221,466 - metI b0198 0.76 -1.1 221,469 - metI b0198 0.77 +0.2 221,511 - metI b0198 0.83 -2.7 221,511 - metI b0198 0.83 -4.1 221,595 - +0.2 221,595 - -0.1 221,619 + -1.2 221,623 + -0.7 221,655 - +0.6 221,680 + -1.5 221,685 + +1.9 221,688 - -1.2 221,802 + metN b0199 0.18 +0.0 221,830 + metN b0199 0.21 -0.3 221,830 + metN b0199 0.21 -0.9 221,846 + metN b0199 0.22 +0.7 221,847 + metN b0199 0.23 -0.2 221,848 + metN b0199 0.23 -0.0 221,856 + metN b0199 0.23 +0.8 221,911 - metN b0199 0.29 -2.2
Or see this region's nucleotide sequence