Experiment: MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4959 and PP_4960 are separated by 212 nucleotides PP_4960 and PP_4961 are separated by 100 nucleotides PP_4961 and PP_4962 are separated by 160 nucleotides PP_4962 and PP_4963 overlap by 1 nucleotides
PP_4959: PP_4959 - diguanylate cyclase, at 5,649,066 to 5,651,192
_4959
PP_4960: PP_4960 - Fructose-bisphosphate aldolase, at 5,651,405 to 5,652,469
_4960
PP_4961: PP_4961 - putative Lipoprotein, at 5,652,570 to 5,652,899
_4961
PP_4962: PP_4962 - conserved exported protein of unknown function, at 5,653,060 to 5,653,311
_4962
PP_4963: PP_4963 - Phosphoglycerate kinase, at 5,653,311 to 5,654,474
_4963
Position (kb)
5651
5652
5653 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5650.510 kb on - strand, within PP_4959 at 5650.534 kb on + strand, within PP_4959 at 5650.544 kb on - strand, within PP_4959 at 5650.544 kb on - strand, within PP_4959 at 5650.849 kb on + strand, within PP_4959 at 5650.849 kb on + strand, within PP_4959 at 5650.849 kb on + strand at 5650.850 kb on - strand, within PP_4959 at 5650.870 kb on + strand, within PP_4959 at 5650.995 kb on + strand at 5650.995 kb on + strand at 5650.995 kb on + strand at 5650.995 kb on + strand at 5650.995 kb on + strand at 5650.995 kb on + strand at 5650.995 kb on + strand at 5650.995 kb on + strand at 5650.996 kb on - strand at 5650.996 kb on - strand at 5650.996 kb on - strand at 5650.996 kb on - strand at 5650.996 kb on - strand at 5650.996 kb on - strand at 5650.996 kb on - strand at 5650.996 kb on - strand at 5650.996 kb on - strand at 5651.050 kb on - strand at 5651.050 kb on - strand at 5651.070 kb on + strand at 5651.070 kb on + strand at 5651.070 kb on + strand at 5651.070 kb on + strand at 5651.071 kb on - strand at 5651.071 kb on - strand at 5651.180 kb on - strand at 5651.206 kb on + strand at 5651.206 kb on + strand at 5651.206 kb on + strand at 5651.206 kb on + strand at 5651.206 kb on + strand at 5651.207 kb on - strand at 5651.207 kb on - strand at 5651.240 kb on + strand at 5651.241 kb on - strand at 5651.243 kb on + strand at 5651.243 kb on + strand at 5651.249 kb on - strand at 5651.249 kb on - strand at 5651.249 kb on - strand at 5651.249 kb on - strand at 5651.303 kb on + strand at 5651.304 kb on - strand at 5651.337 kb on + strand at 5651.395 kb on + strand at 5652.620 kb on - strand, within PP_4961 at 5652.710 kb on - strand, within PP_4961 at 5652.960 kb on + strand at 5652.961 kb on - strand at 5652.961 kb on - strand at 5653.050 kb on + strand at 5653.050 kb on + strand at 5653.050 kb on + strand at 5653.051 kb on - strand at 5653.173 kb on - strand, within PP_4962
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL remove 5,650,510 - PP_4959 0.68 +1.6 5,650,534 + PP_4959 0.69 +1.1 5,650,544 - PP_4959 0.69 +0.9 5,650,544 - PP_4959 0.69 +0.3 5,650,849 + PP_4959 0.84 -0.4 5,650,849 + PP_4959 0.84 +1.1 5,650,849 + +2.5 5,650,850 - PP_4959 0.84 +1.5 5,650,870 + PP_4959 0.85 +0.7 5,650,995 + +3.5 5,650,995 + +1.2 5,650,995 + +1.8 5,650,995 + +1.4 5,650,995 + +1.6 5,650,995 + +1.4 5,650,995 + -0.5 5,650,995 + +1.5 5,650,996 - +2.5 5,650,996 - +1.2 5,650,996 - +1.1 5,650,996 - +1.2 5,650,996 - +1.5 5,650,996 - +1.1 5,650,996 - +0.9 5,650,996 - +0.8 5,650,996 - +0.4 5,651,050 - +0.8 5,651,050 - +0.8 5,651,070 + +1.4 5,651,070 + +1.6 5,651,070 + -0.2 5,651,070 + +1.3 5,651,071 - +1.4 5,651,071 - +0.9 5,651,180 - +1.9 5,651,206 + +1.2 5,651,206 + +1.1 5,651,206 + +1.1 5,651,206 + +0.8 5,651,206 + +0.9 5,651,207 - -0.6 5,651,207 - +0.5 5,651,240 + +1.0 5,651,241 - -0.0 5,651,243 + +1.0 5,651,243 + +0.5 5,651,249 - -0.2 5,651,249 - -0.5 5,651,249 - -0.1 5,651,249 - -0.7 5,651,303 + +0.6 5,651,304 - -1.4 5,651,337 + +1.6 5,651,395 + +1.5 5,652,620 - PP_4961 0.15 -0.9 5,652,710 - PP_4961 0.42 -0.4 5,652,960 + -0.5 5,652,961 - -0.2 5,652,961 - -0.7 5,653,050 + +0.7 5,653,050 + +0.2 5,653,050 + -2.7 5,653,051 - +0.6 5,653,173 - PP_4962 0.45 -0.4
Or see this region's nucleotide sequence