Experiment: MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4697 and PP_4698 overlap by 4 nucleotides PP_4698 and PP_4699 are separated by 262 nucleotides PP_4699 and PP_4700 overlap by 4 nucleotides
PP_4697: PP_4697 - poly(A) polymerase, at 5,338,773 to 5,340,155
_4697
PP_4698: PP_4698 - 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, at 5,340,152 to 5,340,631
_4698
PP_4699: PP_4699 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 5,340,894 to 5,341,694
_4699
PP_4700: PP_4700 - pantothenate synthetase, at 5,341,691 to 5,342,554
_4700
Position (kb)
5340
5341
5342 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5339.952 kb on + strand, within PP_4697 at 5340.167 kb on + strand at 5340.167 kb on + strand at 5340.629 kb on + strand at 5340.629 kb on + strand at 5340.630 kb on - strand at 5340.674 kb on - strand at 5340.674 kb on - strand at 5340.674 kb on - strand at 5340.687 kb on + strand at 5340.735 kb on + strand at 5340.735 kb on + strand at 5340.735 kb on + strand at 5340.735 kb on + strand at 5340.737 kb on + strand at 5340.737 kb on + strand at 5340.738 kb on - strand at 5340.738 kb on - strand at 5340.738 kb on - strand at 5340.738 kb on - strand at 5340.738 kb on - strand at 5340.769 kb on - strand at 5340.769 kb on - strand at 5340.840 kb on + strand at 5340.841 kb on - strand at 5340.841 kb on - strand at 5340.841 kb on - strand at 5340.841 kb on - strand at 5340.841 kb on - strand at 5340.891 kb on + strand at 5340.891 kb on + strand at 5340.892 kb on - strand at 5340.904 kb on + strand at 5340.905 kb on - strand at 5340.911 kb on + strand at 5340.911 kb on + strand at 5340.911 kb on + strand at 5340.912 kb on - strand at 5340.966 kb on + strand at 5340.966 kb on + strand at 5340.966 kb on + strand at 5340.966 kb on + strand at 5341.092 kb on + strand, within PP_4699 at 5341.093 kb on - strand, within PP_4699 at 5341.285 kb on + strand, within PP_4699 at 5341.285 kb on + strand, within PP_4699 at 5341.341 kb on + strand, within PP_4699 at 5341.442 kb on - strand, within PP_4699 at 5341.528 kb on + strand, within PP_4699 at 5341.528 kb on + strand, within PP_4699 at 5341.528 kb on + strand, within PP_4699 at 5341.528 kb on + strand, within PP_4699 at 5341.529 kb on - strand, within PP_4699 at 5341.534 kb on + strand, within PP_4699 at 5341.535 kb on - strand, within PP_4699 at 5341.615 kb on + strand at 5341.635 kb on + strand at 5341.635 kb on + strand at 5341.696 kb on + strand at 5341.696 kb on + strand at 5341.852 kb on + strand, within PP_4700 at 5341.901 kb on + strand, within PP_4700 at 5341.902 kb on - strand, within PP_4700 at 5342.148 kb on - strand at 5342.148 kb on - strand at 5342.148 kb on - strand, within PP_4700 at 5342.148 kb on - strand at 5342.192 kb on - strand, within PP_4700 at 5342.192 kb on - strand, within PP_4700 at 5342.265 kb on - strand, within PP_4700 at 5342.284 kb on + strand, within PP_4700 at 5342.285 kb on - strand at 5342.285 kb on - strand, within PP_4700 at 5342.303 kb on + strand, within PP_4700 at 5342.304 kb on - strand, within PP_4700 at 5342.414 kb on + strand, within PP_4700 at 5342.424 kb on + strand, within PP_4700 at 5342.424 kb on + strand, within PP_4700 at 5342.425 kb on - strand at 5342.425 kb on - strand at 5342.425 kb on - strand at 5342.493 kb on - strand at 5342.526 kb on - strand at 5342.526 kb on - strand at 5342.608 kb on + strand at 5342.609 kb on - strand at 5342.609 kb on - strand at 5342.658 kb on - strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.686 kb on - strand at 5342.687 kb on + strand at 5342.688 kb on - strand at 5342.688 kb on - strand at 5342.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL remove 5,339,952 + PP_4697 0.85 -0.4 5,340,167 + -0.7 5,340,167 + -0.0 5,340,629 + -0.5 5,340,629 + -0.0 5,340,630 - -1.0 5,340,674 - +0.6 5,340,674 - -1.2 5,340,674 - +0.3 5,340,687 + +0.0 5,340,735 + -0.7 5,340,735 + +0.2 5,340,735 + +0.5 5,340,735 + +0.6 5,340,737 + +1.7 5,340,737 + -0.0 5,340,738 - +0.7 5,340,738 - -0.3 5,340,738 - +0.7 5,340,738 - -1.2 5,340,738 - -1.2 5,340,769 - -1.1 5,340,769 - -0.0 5,340,840 + -0.1 5,340,841 - -1.0 5,340,841 - -1.4 5,340,841 - -0.0 5,340,841 - -2.1 5,340,841 - -1.2 5,340,891 + -0.1 5,340,891 + -2.5 5,340,892 - -3.0 5,340,904 + -1.2 5,340,905 - -2.7 5,340,911 + +0.7 5,340,911 + +0.3 5,340,911 + -1.6 5,340,912 - -2.5 5,340,966 + -0.5 5,340,966 + -1.0 5,340,966 + -1.3 5,340,966 + +0.8 5,341,092 + PP_4699 0.25 -0.9 5,341,093 - PP_4699 0.25 -1.5 5,341,285 + PP_4699 0.49 +0.3 5,341,285 + PP_4699 0.49 -2.9 5,341,341 + PP_4699 0.56 +0.3 5,341,442 - PP_4699 0.68 -2.2 5,341,528 + PP_4699 0.79 -1.4 5,341,528 + PP_4699 0.79 -1.4 5,341,528 + PP_4699 0.79 -1.8 5,341,528 + PP_4699 0.79 -0.3 5,341,529 - PP_4699 0.79 -0.5 5,341,534 + PP_4699 0.80 -1.3 5,341,535 - PP_4699 0.80 -2.2 5,341,615 + -1.7 5,341,635 + -1.0 5,341,635 + -2.9 5,341,696 + -0.7 5,341,696 + -1.4 5,341,852 + PP_4700 0.19 -1.8 5,341,901 + PP_4700 0.24 -3.0 5,341,902 - PP_4700 0.24 -2.8 5,342,148 - -1.0 5,342,148 - -1.6 5,342,148 - PP_4700 0.53 -2.0 5,342,148 - -1.5 5,342,192 - PP_4700 0.58 -3.6 5,342,192 - PP_4700 0.58 -2.8 5,342,265 - PP_4700 0.66 -2.4 5,342,284 + PP_4700 0.69 -1.6 5,342,285 - -1.9 5,342,285 - PP_4700 0.69 -3.0 5,342,303 + PP_4700 0.71 -1.3 5,342,304 - PP_4700 0.71 -2.4 5,342,414 + PP_4700 0.84 -2.0 5,342,424 + PP_4700 0.85 -2.2 5,342,424 + PP_4700 0.85 -1.0 5,342,425 - -2.0 5,342,425 - -2.4 5,342,425 - -1.9 5,342,493 - -2.2 5,342,526 - +0.5 5,342,526 - +1.3 5,342,608 + +0.7 5,342,609 - +1.2 5,342,609 - -0.9 5,342,658 - +0.7 5,342,685 + -2.3 5,342,685 + -0.2 5,342,685 + +2.3 5,342,685 + -1.4 5,342,685 + -1.2 5,342,685 + -0.6 5,342,686 - -0.6 5,342,687 + +1.3 5,342,688 - +0.0 5,342,688 - -0.7 5,342,688 - -0.1
Or see this region's nucleotide sequence