Experiment: MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4697 and PP_4698 overlap by 4 nucleotides PP_4698 and PP_4699 are separated by 262 nucleotides
PP_4697: PP_4697 - poly(A) polymerase, at 5,338,773 to 5,340,155
_4697
PP_4698: PP_4698 - 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, at 5,340,152 to 5,340,631
_4698
PP_4699: PP_4699 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 5,340,894 to 5,341,694
_4699
Position (kb) 5340
5341 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5339.262 kb on + strand, within PP_4697 at 5339.265 kb on + strand, within PP_4697 at 5339.265 kb on + strand, within PP_4697 at 5339.265 kb on + strand, within PP_4697 at 5339.265 kb on + strand, within PP_4697 at 5339.439 kb on + strand, within PP_4697 at 5339.439 kb on + strand, within PP_4697 at 5339.439 kb on + strand, within PP_4697 at 5339.952 kb on + strand, within PP_4697 at 5340.167 kb on + strand at 5340.167 kb on + strand at 5340.629 kb on + strand at 5340.629 kb on + strand at 5340.630 kb on - strand at 5340.674 kb on - strand at 5340.674 kb on - strand at 5340.674 kb on - strand at 5340.687 kb on + strand at 5340.735 kb on + strand at 5340.735 kb on + strand at 5340.735 kb on + strand at 5340.735 kb on + strand at 5340.737 kb on + strand at 5340.737 kb on + strand at 5340.738 kb on - strand at 5340.738 kb on - strand at 5340.738 kb on - strand at 5340.738 kb on - strand at 5340.738 kb on - strand at 5340.769 kb on - strand at 5340.769 kb on - strand at 5340.840 kb on + strand at 5340.841 kb on - strand at 5340.841 kb on - strand at 5340.841 kb on - strand at 5340.841 kb on - strand at 5340.841 kb on - strand at 5340.891 kb on + strand at 5340.891 kb on + strand at 5340.892 kb on - strand at 5340.904 kb on + strand at 5340.905 kb on - strand at 5340.911 kb on + strand at 5340.911 kb on + strand at 5340.911 kb on + strand at 5340.912 kb on - strand at 5340.966 kb on + strand at 5340.966 kb on + strand at 5340.966 kb on + strand at 5340.966 kb on + strand at 5341.092 kb on + strand, within PP_4699 at 5341.093 kb on - strand, within PP_4699 at 5341.285 kb on + strand, within PP_4699 at 5341.285 kb on + strand, within PP_4699 at 5341.341 kb on + strand, within PP_4699 at 5341.442 kb on - strand, within PP_4699 at 5341.528 kb on + strand, within PP_4699 at 5341.528 kb on + strand, within PP_4699 at 5341.528 kb on + strand, within PP_4699 at 5341.528 kb on + strand, within PP_4699 at 5341.529 kb on - strand, within PP_4699 at 5341.534 kb on + strand, within PP_4699 at 5341.535 kb on - strand, within PP_4699 at 5341.615 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL remove 5,339,262 + PP_4697 0.35 +1.3 5,339,265 + PP_4697 0.36 -0.8 5,339,265 + PP_4697 0.36 -1.4 5,339,265 + PP_4697 0.36 +0.6 5,339,265 + PP_4697 0.36 -0.1 5,339,439 + PP_4697 0.48 +1.5 5,339,439 + PP_4697 0.48 -2.1 5,339,439 + PP_4697 0.48 -1.7 5,339,952 + PP_4697 0.85 -0.4 5,340,167 + -0.7 5,340,167 + -0.0 5,340,629 + -0.5 5,340,629 + -0.0 5,340,630 - -1.0 5,340,674 - +0.6 5,340,674 - -1.2 5,340,674 - +0.3 5,340,687 + +0.0 5,340,735 + -0.7 5,340,735 + +0.2 5,340,735 + +0.5 5,340,735 + +0.6 5,340,737 + +1.7 5,340,737 + -0.0 5,340,738 - +0.7 5,340,738 - -0.3 5,340,738 - +0.7 5,340,738 - -1.2 5,340,738 - -1.2 5,340,769 - -1.1 5,340,769 - -0.0 5,340,840 + -0.1 5,340,841 - -1.0 5,340,841 - -1.4 5,340,841 - -0.0 5,340,841 - -2.1 5,340,841 - -1.2 5,340,891 + -0.1 5,340,891 + -2.5 5,340,892 - -3.0 5,340,904 + -1.2 5,340,905 - -2.7 5,340,911 + +0.7 5,340,911 + +0.3 5,340,911 + -1.6 5,340,912 - -2.5 5,340,966 + -0.5 5,340,966 + -1.0 5,340,966 + -1.3 5,340,966 + +0.8 5,341,092 + PP_4699 0.25 -0.9 5,341,093 - PP_4699 0.25 -1.5 5,341,285 + PP_4699 0.49 +0.3 5,341,285 + PP_4699 0.49 -2.9 5,341,341 + PP_4699 0.56 +0.3 5,341,442 - PP_4699 0.68 -2.2 5,341,528 + PP_4699 0.79 -1.4 5,341,528 + PP_4699 0.79 -1.4 5,341,528 + PP_4699 0.79 -1.8 5,341,528 + PP_4699 0.79 -0.3 5,341,529 - PP_4699 0.79 -0.5 5,341,534 + PP_4699 0.80 -1.3 5,341,535 - PP_4699 0.80 -2.2 5,341,615 + -1.7
Or see this region's nucleotide sequence