Strain Fitness in Pseudomonas putida KT2440 around PP_4586

Experiment: MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4584 and PP_4585 are separated by 71 nucleotidesPP_4585 and PP_4586 are separated by 114 nucleotidesPP_4586 and PP_4587 are separated by 41 nucleotidesPP_4587 and PP_4588 are separated by 145 nucleotides PP_4584: PP_4584 - putative endonuclease, at 5,204,196 to 5,204,567 _4584 PP_4585: PP_4585 - RNA methyltransferase, TrmH family, at 5,204,639 to 5,205,109 _4585 PP_4586: PP_4586 - conserved exported protein of unknown function, at 5,205,224 to 5,206,432 _4586 PP_4587: PP_4587 - conserved protein of unknown function, at 5,206,474 to 5,206,923 _4587 PP_4588: PP_4588 - putative oxidoreductase (nitroreductase family), at 5,207,069 to 5,207,662 _4588 Position (kb) 5205 5206 5207Strain fitness (log2 ratio) -2 -1 0 1 2at 5204.302 kb on - strand, within PP_4584at 5204.302 kb on - strand, within PP_4584at 5204.433 kb on + strand, within PP_4584at 5204.433 kb on + strand, within PP_4584at 5204.460 kb on + strand, within PP_4584at 5204.475 kb on + strand, within PP_4584at 5204.519 kb on + strand, within PP_4584at 5204.519 kb on + strand, within PP_4584at 5204.604 kb on + strandat 5204.604 kb on + strandat 5204.605 kb on - strandat 5204.606 kb on + strandat 5204.654 kb on + strandat 5204.726 kb on + strand, within PP_4585at 5204.726 kb on + strand, within PP_4585at 5204.726 kb on + strand, within PP_4585at 5204.727 kb on - strand, within PP_4585at 5204.747 kb on + strand, within PP_4585at 5204.747 kb on + strand, within PP_4585at 5204.748 kb on - strand, within PP_4585at 5204.762 kb on + strand, within PP_4585at 5204.763 kb on - strand, within PP_4585at 5204.763 kb on - strand, within PP_4585at 5204.807 kb on + strand, within PP_4585at 5204.807 kb on + strand, within PP_4585at 5204.808 kb on - strand, within PP_4585at 5204.887 kb on - strand, within PP_4585at 5204.887 kb on - strand, within PP_4585at 5204.912 kb on + strand, within PP_4585at 5204.912 kb on + strand, within PP_4585at 5204.912 kb on + strand, within PP_4585at 5204.932 kb on + strand, within PP_4585at 5204.933 kb on - strand, within PP_4585at 5204.936 kb on + strand, within PP_4585at 5204.937 kb on - strand, within PP_4585at 5204.937 kb on - strand, within PP_4585at 5204.937 kb on - strand, within PP_4585at 5204.937 kb on - strand, within PP_4585at 5204.998 kb on + strand, within PP_4585at 5204.998 kb on + strand, within PP_4585at 5204.998 kb on + strand, within PP_4585at 5204.999 kb on - strand, within PP_4585at 5204.999 kb on - strand, within PP_4585at 5204.999 kb on - strand, within PP_4585at 5204.999 kb on - strand, within PP_4585at 5204.999 kb on - strand, within PP_4585at 5205.002 kb on + strand, within PP_4585at 5205.002 kb on + strand, within PP_4585at 5205.002 kb on + strand, within PP_4585at 5205.002 kb on + strand, within PP_4585at 5205.002 kb on + strand, within PP_4585at 5205.003 kb on - strand, within PP_4585at 5205.003 kb on - strand, within PP_4585at 5205.003 kb on - strand, within PP_4585at 5205.003 kb on - strand, within PP_4585at 5205.003 kb on - strand, within PP_4585at 5205.003 kb on - strand, within PP_4585at 5205.056 kb on + strand, within PP_4585at 5205.056 kb on + strand, within PP_4585at 5205.056 kb on + strand, within PP_4585at 5205.056 kb on + strand, within PP_4585at 5205.056 kb on + strand, within PP_4585at 5205.057 kb on - strand, within PP_4585at 5205.083 kb on - strandat 5205.108 kb on - strandat 5205.176 kb on + strandat 5205.176 kb on + strandat 5205.225 kb on + strandat 5205.335 kb on + strandat 5205.335 kb on + strandat 5205.335 kb on + strandat 5205.336 kb on - strandat 5205.336 kb on - strandat 5205.336 kb on - strandat 5205.336 kb on - strandat 5205.530 kb on + strand, within PP_4586at 5205.546 kb on + strand, within PP_4586at 5205.549 kb on + strand, within PP_4586at 5205.550 kb on - strand, within PP_4586at 5205.567 kb on - strand, within PP_4586at 5205.638 kb on - strand, within PP_4586at 5205.678 kb on + strand, within PP_4586at 5205.701 kb on - strand, within PP_4586at 5205.773 kb on + strand, within PP_4586at 5205.773 kb on + strand, within PP_4586at 5205.773 kb on + strand, within PP_4586at 5205.774 kb on - strand, within PP_4586at 5205.774 kb on - strand, within PP_4586at 5206.122 kb on + strand, within PP_4586at 5206.123 kb on - strand, within PP_4586at 5206.161 kb on + strand, within PP_4586at 5206.162 kb on - strand, within PP_4586at 5206.162 kb on - strand, within PP_4586at 5206.199 kb on - strand, within PP_4586at 5206.199 kb on - strand, within PP_4586at 5206.248 kb on + strandat 5206.248 kb on + strand, within PP_4586at 5206.249 kb on - strand, within PP_4586at 5206.249 kb on - strand, within PP_4586at 5206.357 kb on - strandat 5206.357 kb on - strandat 5206.539 kb on + strand, within PP_4587at 5206.539 kb on + strand, within PP_4587at 5206.539 kb on + strand, within PP_4587at 5206.539 kb on + strand, within PP_4587at 5206.539 kb on + strand, within PP_4587at 5206.540 kb on - strandat 5206.540 kb on - strand, within PP_4587at 5206.540 kb on - strand, within PP_4587at 5206.540 kb on - strand, within PP_4587at 5206.650 kb on + strand, within PP_4587at 5206.750 kb on - strand, within PP_4587at 5206.784 kb on + strand, within PP_4587at 5206.784 kb on + strand, within PP_4587at 5206.785 kb on - strand, within PP_4587at 5206.785 kb on - strand, within PP_4587at 5206.785 kb on - strand, within PP_4587at 5206.785 kb on - strand, within PP_4587at 5206.787 kb on + strand, within PP_4587at 5206.787 kb on + strand, within PP_4587at 5206.787 kb on + strand, within PP_4587at 5206.788 kb on - strand, within PP_4587at 5206.788 kb on - strand, within PP_4587at 5206.788 kb on - strand, within PP_4587at 5206.788 kb on - strand, within PP_4587at 5207.032 kb on + strandat 5207.032 kb on + strandat 5207.033 kb on - strandat 5207.213 kb on + strand, within PP_4588at 5207.213 kb on + strand, within PP_4588at 5207.215 kb on + strand, within PP_4588at 5207.215 kb on + strand, within PP_4588at 5207.215 kb on + strand, within PP_4588at 5207.240 kb on + strand, within PP_4588at 5207.240 kb on + strand, within PP_4588at 5207.240 kb on + strand, within PP_4588at 5207.241 kb on - strandat 5207.274 kb on - strand, within PP_4588

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Per-strain Table

Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL
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5,204,302 - PP_4584 0.28 +0.4
5,204,302 - PP_4584 0.28 -1.2
5,204,433 + PP_4584 0.64 -0.4
5,204,433 + PP_4584 0.64 +0.2
5,204,460 + PP_4584 0.71 -1.2
5,204,475 + PP_4584 0.75 +0.1
5,204,519 + PP_4584 0.87 +1.1
5,204,519 + PP_4584 0.87 -0.1
5,204,604 + -0.4
5,204,604 + +0.5
5,204,605 - +0.1
5,204,606 + -1.2
5,204,654 + -0.7
5,204,726 + PP_4585 0.18 -0.0
5,204,726 + PP_4585 0.18 -0.0
5,204,726 + PP_4585 0.18 -0.9
5,204,727 - PP_4585 0.19 +0.2
5,204,747 + PP_4585 0.23 +1.2
5,204,747 + PP_4585 0.23 -0.8
5,204,748 - PP_4585 0.23 +0.1
5,204,762 + PP_4585 0.26 +2.2
5,204,763 - PP_4585 0.26 +0.0
5,204,763 - PP_4585 0.26 +0.6
5,204,807 + PP_4585 0.36 +0.5
5,204,807 + PP_4585 0.36 +1.0
5,204,808 - PP_4585 0.36 -0.6
5,204,887 - PP_4585 0.53 -1.6
5,204,887 - PP_4585 0.53 -2.3
5,204,912 + PP_4585 0.58 +0.8
5,204,912 + PP_4585 0.58 +0.4
5,204,912 + PP_4585 0.58 -0.5
5,204,932 + PP_4585 0.62 -1.0
5,204,933 - PP_4585 0.62 -0.4
5,204,936 + PP_4585 0.63 -0.6
5,204,937 - PP_4585 0.63 -0.1
5,204,937 - PP_4585 0.63 +0.1
5,204,937 - PP_4585 0.63 +1.2
5,204,937 - PP_4585 0.63 +0.2
5,204,998 + PP_4585 0.76 +1.2
5,204,998 + PP_4585 0.76 -1.6
5,204,998 + PP_4585 0.76 -2.5
5,204,999 - PP_4585 0.76 +0.2
5,204,999 - PP_4585 0.76 +1.7
5,204,999 - PP_4585 0.76 -0.9
5,204,999 - PP_4585 0.76 -0.9
5,204,999 - PP_4585 0.76 -1.2
5,205,002 + PP_4585 0.77 +0.5
5,205,002 + PP_4585 0.77 +0.5
5,205,002 + PP_4585 0.77 -1.0
5,205,002 + PP_4585 0.77 +0.1
5,205,002 + PP_4585 0.77 +2.2
5,205,003 - PP_4585 0.77 -0.0
5,205,003 - PP_4585 0.77 -1.7
5,205,003 - PP_4585 0.77 +0.3
5,205,003 - PP_4585 0.77 -1.5
5,205,003 - PP_4585 0.77 -1.3
5,205,003 - PP_4585 0.77 +0.7
5,205,056 + PP_4585 0.89 +0.2
5,205,056 + PP_4585 0.89 +1.1
5,205,056 + PP_4585 0.89 +0.4
5,205,056 + PP_4585 0.89 +1.0
5,205,056 + PP_4585 0.89 -0.1
5,205,057 - PP_4585 0.89 -0.6
5,205,083 - -0.7
5,205,108 - +0.2
5,205,176 + -0.3
5,205,176 + -1.1
5,205,225 + -1.9
5,205,335 + -0.2
5,205,335 + +0.6
5,205,335 + -0.3
5,205,336 - -0.3
5,205,336 - -0.0
5,205,336 - -0.6
5,205,336 - +0.2
5,205,530 + PP_4586 0.25 +1.8
5,205,546 + PP_4586 0.27 -0.6
5,205,549 + PP_4586 0.27 -2.0
5,205,550 - PP_4586 0.27 -0.9
5,205,567 - PP_4586 0.28 -0.0
5,205,638 - PP_4586 0.34 -2.3
5,205,678 + PP_4586 0.38 -1.0
5,205,701 - PP_4586 0.39 -1.9
5,205,773 + PP_4586 0.45 +0.2
5,205,773 + PP_4586 0.45 -0.6
5,205,773 + PP_4586 0.45 -0.9
5,205,774 - PP_4586 0.45 -0.9
5,205,774 - PP_4586 0.45 -1.1
5,206,122 + PP_4586 0.74 +1.2
5,206,123 - PP_4586 0.74 +0.4
5,206,161 + PP_4586 0.78 +0.1
5,206,162 - PP_4586 0.78 -0.6
5,206,162 - PP_4586 0.78 -1.8
5,206,199 - PP_4586 0.81 -0.9
5,206,199 - PP_4586 0.81 -0.4
5,206,248 + -1.1
5,206,248 + PP_4586 0.85 +0.2
5,206,249 - PP_4586 0.85 +1.1
5,206,249 - PP_4586 0.85 -0.2
5,206,357 - -0.5
5,206,357 - -0.1
5,206,539 + PP_4587 0.14 +1.5
5,206,539 + PP_4587 0.14 -0.6
5,206,539 + PP_4587 0.14 -0.2
5,206,539 + PP_4587 0.14 +0.8
5,206,539 + PP_4587 0.14 +0.1
5,206,540 - +2.2
5,206,540 - PP_4587 0.15 +0.6
5,206,540 - PP_4587 0.15 -0.2
5,206,540 - PP_4587 0.15 -0.8
5,206,650 + PP_4587 0.39 -0.1
5,206,750 - PP_4587 0.61 +0.2
5,206,784 + PP_4587 0.69 +0.1
5,206,784 + PP_4587 0.69 +1.8
5,206,785 - PP_4587 0.69 +1.3
5,206,785 - PP_4587 0.69 +0.2
5,206,785 - PP_4587 0.69 +1.2
5,206,785 - PP_4587 0.69 -1.7
5,206,787 + PP_4587 0.70 +0.5
5,206,787 + PP_4587 0.70 +0.2
5,206,787 + PP_4587 0.70 +0.3
5,206,788 - PP_4587 0.70 -0.1
5,206,788 - PP_4587 0.70 +0.5
5,206,788 - PP_4587 0.70 -0.5
5,206,788 - PP_4587 0.70 +0.4
5,207,032 + +0.2
5,207,032 + +1.1
5,207,033 - +0.0
5,207,213 + PP_4588 0.24 +0.0
5,207,213 + PP_4588 0.24 -0.0
5,207,215 + PP_4588 0.25 +0.5
5,207,215 + PP_4588 0.25 -1.3
5,207,215 + PP_4588 0.25 -0.1
5,207,240 + PP_4588 0.29 -0.6
5,207,240 + PP_4588 0.29 -1.3
5,207,240 + PP_4588 0.29 +0.2
5,207,241 - +1.7
5,207,274 - PP_4588 0.35 -0.5

Or see this region's nucleotide sequence