Strain Fitness in Pseudomonas putida KT2440 around PP_0665

Experiment: MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0664 and PP_0665 are separated by 12 nucleotidesPP_0665 and PP_0666 are separated by 16 nucleotidesPP_0666 and PP_0667 are separated by 170 nucleotides PP_0664: PP_0664 - homoserine dehydrogenase, at 772,338 to 773,381 _0664 PP_0665: PP_0665 - putative glyceraldehyde-3-phosphate dehydrogenase, at 773,394 to 774,812 _0665 PP_0666: PP_0666 - conserved protein of unknown function, at 774,829 to 775,191 _0666 PP_0667: PP_0667 - RNA polymerase sigma-70 factor, ECF subfamily, at 775,362 to 775,898 _0667 Position (kb) 773 774 775Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 772.788 kb on + strand, within PP_0664at 772.827 kb on - strand, within PP_0664at 772.862 kb on - strand, within PP_0664at 772.886 kb on + strand, within PP_0664at 772.886 kb on + strand, within PP_0664at 772.887 kb on - strand, within PP_0664at 772.892 kb on + strand, within PP_0664at 772.892 kb on + strand, within PP_0664at 772.893 kb on - strand, within PP_0664at 772.893 kb on - strand, within PP_0664at 772.893 kb on - strand, within PP_0664at 772.896 kb on + strand, within PP_0664at 772.896 kb on + strand, within PP_0664at 772.896 kb on + strand, within PP_0664at 772.896 kb on + strand, within PP_0664at 772.896 kb on + strand, within PP_0664at 772.896 kb on + strand, within PP_0664at 772.897 kb on - strand, within PP_0664at 772.897 kb on - strand, within PP_0664at 772.897 kb on - strand, within PP_0664at 772.897 kb on - strand, within PP_0664at 773.008 kb on + strand, within PP_0664at 773.008 kb on + strand, within PP_0664at 773.008 kb on + strand, within PP_0664at 773.009 kb on - strand, within PP_0664at 773.009 kb on - strand, within PP_0664at 773.009 kb on - strand, within PP_0664at 773.183 kb on - strand, within PP_0664at 773.410 kb on + strandat 773.451 kb on + strandat 773.451 kb on + strandat 773.451 kb on + strandat 773.451 kb on + strandat 773.452 kb on - strandat 773.452 kb on - strandat 773.452 kb on - strandat 773.452 kb on - strandat 773.766 kb on + strand, within PP_0665at 773.766 kb on + strand, within PP_0665at 773.766 kb on + strand, within PP_0665at 773.766 kb on + strand, within PP_0665at 773.766 kb on + strand, within PP_0665at 773.766 kb on + strand, within PP_0665at 773.767 kb on - strand, within PP_0665at 773.767 kb on - strand, within PP_0665at 773.767 kb on - strand, within PP_0665at 773.767 kb on - strand, within PP_0665at 773.832 kb on - strand, within PP_0665at 773.956 kb on - strand, within PP_0665at 773.996 kb on - strand, within PP_0665at 774.253 kb on + strand, within PP_0665at 774.254 kb on - strand, within PP_0665at 774.268 kb on - strand, within PP_0665at 774.268 kb on - strand, within PP_0665at 774.456 kb on + strand, within PP_0665at 774.589 kb on - strand, within PP_0665at 774.589 kb on - strand, within PP_0665at 774.650 kb on + strand, within PP_0665at 774.667 kb on + strand, within PP_0665at 774.667 kb on + strand, within PP_0665at 774.667 kb on + strand, within PP_0665at 774.667 kb on + strand, within PP_0665at 774.668 kb on - strand, within PP_0665at 774.668 kb on - strand, within PP_0665at 774.726 kb on + strandat 774.726 kb on + strandat 774.726 kb on + strandat 774.727 kb on - strandat 774.727 kb on - strandat 774.727 kb on - strandat 774.763 kb on - strandat 774.822 kb on + strandat 774.823 kb on - strandat 774.823 kb on - strandat 774.823 kb on - strandat 775.066 kb on + strand, within PP_0666at 775.066 kb on + strand, within PP_0666at 775.066 kb on + strand, within PP_0666at 775.067 kb on - strand, within PP_0666at 775.067 kb on - strand, within PP_0666at 775.136 kb on - strand, within PP_0666at 775.225 kb on + strandat 775.413 kb on + strandat 775.414 kb on - strandat 775.498 kb on + strand, within PP_0667at 775.557 kb on + strand, within PP_0667at 775.558 kb on - strand, within PP_0667at 775.568 kb on + strand, within PP_0667at 775.568 kb on + strand, within PP_0667at 775.568 kb on + strand, within PP_0667at 775.568 kb on + strand, within PP_0667at 775.568 kb on + strand, within PP_0667at 775.568 kb on + strand, within PP_0667at 775.569 kb on - strand, within PP_0667at 775.727 kb on + strand, within PP_0667at 775.727 kb on + strand, within PP_0667at 775.728 kb on - strand, within PP_0667

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Per-strain Table

Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL
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772,788 + PP_0664 0.43 -0.7
772,827 - PP_0664 0.47 +0.7
772,862 - PP_0664 0.50 -0.4
772,886 + PP_0664 0.52 -1.4
772,886 + PP_0664 0.52 +0.2
772,887 - PP_0664 0.53 -0.8
772,892 + PP_0664 0.53 -1.9
772,892 + PP_0664 0.53 +0.4
772,893 - PP_0664 0.53 +0.3
772,893 - PP_0664 0.53 +0.0
772,893 - PP_0664 0.53 +0.7
772,896 + PP_0664 0.53 -1.4
772,896 + PP_0664 0.53 -0.0
772,896 + PP_0664 0.53 -2.1
772,896 + PP_0664 0.53 +0.0
772,896 + PP_0664 0.53 -0.3
772,896 + PP_0664 0.53 +0.1
772,897 - PP_0664 0.54 -1.0
772,897 - PP_0664 0.54 +1.5
772,897 - PP_0664 0.54 +0.1
772,897 - PP_0664 0.54 -0.7
773,008 + PP_0664 0.64 +0.1
773,008 + PP_0664 0.64 -0.0
773,008 + PP_0664 0.64 +0.1
773,009 - PP_0664 0.64 +0.7
773,009 - PP_0664 0.64 -2.9
773,009 - PP_0664 0.64 -0.7
773,183 - PP_0664 0.81 +0.1
773,410 + -0.4
773,451 + +0.4
773,451 + +0.7
773,451 + -0.4
773,451 + +1.5
773,452 - -1.8
773,452 - +0.3
773,452 - -0.4
773,452 - -1.7
773,766 + PP_0665 0.26 -0.8
773,766 + PP_0665 0.26 -1.4
773,766 + PP_0665 0.26 -2.5
773,766 + PP_0665 0.26 -2.0
773,766 + PP_0665 0.26 -2.1
773,766 + PP_0665 0.26 +0.5
773,767 - PP_0665 0.26 -1.2
773,767 - PP_0665 0.26 -0.3
773,767 - PP_0665 0.26 -0.1
773,767 - PP_0665 0.26 +0.3
773,832 - PP_0665 0.31 +0.7
773,956 - PP_0665 0.40 -0.3
773,996 - PP_0665 0.42 -0.4
774,253 + PP_0665 0.61 +1.8
774,254 - PP_0665 0.61 +0.5
774,268 - PP_0665 0.62 +0.2
774,268 - PP_0665 0.62 -0.9
774,456 + PP_0665 0.75 -0.3
774,589 - PP_0665 0.84 +0.3
774,589 - PP_0665 0.84 +2.3
774,650 + PP_0665 0.89 +0.1
774,667 + PP_0665 0.90 -0.4
774,667 + PP_0665 0.90 -1.2
774,667 + PP_0665 0.90 -0.6
774,667 + PP_0665 0.90 +0.2
774,668 - PP_0665 0.90 -0.6
774,668 - PP_0665 0.90 -1.4
774,726 + -0.3
774,726 + +0.2
774,726 + -0.3
774,727 - +0.5
774,727 - +0.1
774,727 - +0.9
774,763 - +0.5
774,822 + -1.0
774,823 - +0.1
774,823 - +1.0
774,823 - -0.3
775,066 + PP_0666 0.65 +0.6
775,066 + PP_0666 0.65 +1.9
775,066 + PP_0666 0.65 +1.9
775,067 - PP_0666 0.66 +0.8
775,067 - PP_0666 0.66 -0.5
775,136 - PP_0666 0.85 -0.8
775,225 + -1.3
775,413 + -0.8
775,414 - +0.8
775,498 + PP_0667 0.25 +0.6
775,557 + PP_0667 0.36 -0.0
775,558 - PP_0667 0.36 -0.5
775,568 + PP_0667 0.38 +0.2
775,568 + PP_0667 0.38 -1.0
775,568 + PP_0667 0.38 +0.9
775,568 + PP_0667 0.38 -0.0
775,568 + PP_0667 0.38 -1.3
775,568 + PP_0667 0.38 +0.5
775,569 - PP_0667 0.39 +0.9
775,727 + PP_0667 0.68 -0.6
775,727 + PP_0667 0.68 -0.8
775,728 - PP_0667 0.68 -0.8

Or see this region's nucleotide sequence