Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4843 and PP_4844 overlap by 4 nucleotides PP_4844 and PP_4845 are separated by 153 nucleotides PP_4845 and PP_4846 are separated by 31 nucleotides
PP_4843: PP_4843 - putative Urea ABC transporter, permease protein, at 5,511,289 to 5,512,368
_4843
PP_4844: PP_4844 - putative Urea ABC transporter, ATP-binding protein, at 5,512,365 to 5,513,222
_4844
PP_4845: PP_4845 - putative Urea ABC transporter, ATP-binding protein, at 5,513,376 to 5,514,074
_4845
PP_4846: PP_4846 - L-amino acid N-acyltransferase, at 5,514,106 to 5,514,621
_4846
Position (kb) 5512
5513
5514 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5511.433 kb on + strand, within PP_4843 at 5511.434 kb on - strand, within PP_4843 at 5511.434 kb on - strand, within PP_4843 at 5511.466 kb on + strand, within PP_4843 at 5511.571 kb on + strand, within PP_4843 at 5511.571 kb on + strand, within PP_4843 at 5511.571 kb on + strand at 5511.572 kb on - strand, within PP_4843 at 5511.647 kb on - strand, within PP_4843 at 5511.760 kb on + strand, within PP_4843 at 5511.761 kb on - strand, within PP_4843 at 5511.946 kb on + strand, within PP_4843 at 5511.946 kb on + strand, within PP_4843 at 5512.033 kb on + strand, within PP_4843 at 5512.034 kb on - strand, within PP_4843 at 5512.106 kb on - strand, within PP_4843 at 5512.318 kb on + strand at 5512.318 kb on + strand at 5512.318 kb on + strand at 5512.318 kb on + strand at 5512.560 kb on + strand, within PP_4844 at 5512.561 kb on - strand, within PP_4844 at 5512.631 kb on - strand, within PP_4844 at 5512.631 kb on - strand, within PP_4844 at 5512.631 kb on - strand, within PP_4844 at 5512.668 kb on + strand, within PP_4844 at 5512.669 kb on - strand, within PP_4844 at 5512.707 kb on + strand, within PP_4844 at 5513.208 kb on + strand at 5513.209 kb on - strand at 5513.209 kb on - strand at 5513.277 kb on + strand at 5513.277 kb on + strand at 5513.278 kb on - strand at 5513.278 kb on - strand at 5513.288 kb on + strand at 5513.288 kb on + strand at 5513.289 kb on - strand at 5513.314 kb on - strand at 5513.325 kb on + strand at 5513.403 kb on + strand at 5513.403 kb on + strand at 5513.403 kb on + strand at 5513.404 kb on - strand at 5513.406 kb on + strand at 5513.406 kb on + strand at 5513.407 kb on - strand at 5513.407 kb on - strand at 5513.407 kb on - strand at 5513.613 kb on + strand, within PP_4845 at 5513.613 kb on + strand, within PP_4845 at 5513.613 kb on + strand, within PP_4845 at 5513.725 kb on - strand, within PP_4845 at 5513.725 kb on - strand, within PP_4845 at 5513.955 kb on + strand, within PP_4845 at 5513.956 kb on - strand, within PP_4845 at 5514.052 kb on + strand at 5514.052 kb on + strand at 5514.052 kb on + strand at 5514.053 kb on - strand at 5514.053 kb on - strand at 5514.064 kb on + strand at 5514.072 kb on + strand at 5514.073 kb on - strand at 5514.152 kb on + strand at 5514.163 kb on + strand, within PP_4846 at 5514.164 kb on - strand, within PP_4846 at 5514.164 kb on - strand, within PP_4846 at 5514.164 kb on - strand, within PP_4846 at 5514.164 kb on - strand, within PP_4846
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 5,511,433 + PP_4843 0.13 +0.0 5,511,434 - PP_4843 0.13 -2.9 5,511,434 - PP_4843 0.13 +0.4 5,511,466 + PP_4843 0.16 +0.1 5,511,571 + PP_4843 0.26 +0.4 5,511,571 + PP_4843 0.26 +0.4 5,511,571 + +2.1 5,511,572 - PP_4843 0.26 +2.1 5,511,647 - PP_4843 0.33 -0.8 5,511,760 + PP_4843 0.44 -0.5 5,511,761 - PP_4843 0.44 +1.6 5,511,946 + PP_4843 0.61 -0.1 5,511,946 + PP_4843 0.61 +0.2 5,512,033 + PP_4843 0.69 +0.1 5,512,034 - PP_4843 0.69 +0.3 5,512,106 - PP_4843 0.76 -0.2 5,512,318 + -1.2 5,512,318 + +1.1 5,512,318 + -0.7 5,512,318 + -1.1 5,512,560 + PP_4844 0.23 -0.0 5,512,561 - PP_4844 0.23 +1.5 5,512,631 - PP_4844 0.31 +2.1 5,512,631 - PP_4844 0.31 -0.2 5,512,631 - PP_4844 0.31 +1.3 5,512,668 + PP_4844 0.35 +1.9 5,512,669 - PP_4844 0.35 -0.9 5,512,707 + PP_4844 0.40 +0.4 5,513,208 + -0.1 5,513,209 - -0.7 5,513,209 - +0.1 5,513,277 + -0.2 5,513,277 + +0.1 5,513,278 - -0.7 5,513,278 - +0.1 5,513,288 + +0.4 5,513,288 + -1.9 5,513,289 - -2.5 5,513,314 - -0.1 5,513,325 + +0.1 5,513,403 + +0.1 5,513,403 + +0.2 5,513,403 + -0.3 5,513,404 - +0.2 5,513,406 + -0.7 5,513,406 + -0.2 5,513,407 - +0.6 5,513,407 - +0.1 5,513,407 - -1.2 5,513,613 + PP_4845 0.34 -1.1 5,513,613 + PP_4845 0.34 +0.1 5,513,613 + PP_4845 0.34 -0.9 5,513,725 - PP_4845 0.50 +1.6 5,513,725 - PP_4845 0.50 +1.7 5,513,955 + PP_4845 0.83 -0.3 5,513,956 - PP_4845 0.83 -1.1 5,514,052 + +1.7 5,514,052 + +0.0 5,514,052 + +0.1 5,514,053 - +0.2 5,514,053 - -1.6 5,514,064 + +0.8 5,514,072 + +0.1 5,514,073 - +0.2 5,514,152 + +0.7 5,514,163 + PP_4846 0.11 -0.5 5,514,164 - PP_4846 0.11 -0.1 5,514,164 - PP_4846 0.11 +0.3 5,514,164 - PP_4846 0.11 +0.4 5,514,164 - PP_4846 0.11 -0.5
Or see this region's nucleotide sequence