Strain Fitness in Pseudomonas putida KT2440 around PP_0544

Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0543 and PP_0544 are separated by 46 nucleotidesPP_0544 and PP_0545 are separated by 227 nucleotides PP_0543: PP_0543 - ethanolamine ammonia-lyase, alpha-subunit, at 628,803 to 630,197 _0543 PP_0544: PP_0544 - Ethanolamine transporter, at 630,244 to 631,692 _0544 PP_0545: PP_0545 - aldehyde dehydrogenase, at 631,920 to 633,440 _0545 Position (kb) 630 631 632Strain fitness (log2 ratio) -2 -1 0 1 2at 629.257 kb on + strand, within PP_0543at 629.258 kb on - strand, within PP_0543at 629.258 kb on - strand, within PP_0543at 629.338 kb on + strand, within PP_0543at 629.389 kb on + strand, within PP_0543at 629.389 kb on + strand, within PP_0543at 629.389 kb on + strand, within PP_0543at 629.390 kb on - strand, within PP_0543at 629.549 kb on - strand, within PP_0543at 629.648 kb on - strand, within PP_0543at 629.648 kb on - strand, within PP_0543at 630.197 kb on + strandat 630.323 kb on + strandat 630.324 kb on - strandat 630.324 kb on - strandat 630.335 kb on + strandat 630.336 kb on - strandat 630.336 kb on - strandat 630.336 kb on - strandat 630.343 kb on + strandat 630.344 kb on - strandat 630.348 kb on - strandat 630.353 kb on + strandat 630.353 kb on + strandat 630.353 kb on + strandat 630.353 kb on + strandat 630.354 kb on - strandat 630.467 kb on + strand, within PP_0544at 630.530 kb on + strand, within PP_0544at 630.530 kb on + strand, within PP_0544at 630.530 kb on + strand, within PP_0544at 630.531 kb on - strand, within PP_0544at 630.531 kb on - strand, within PP_0544at 630.531 kb on - strand, within PP_0544at 630.714 kb on - strand, within PP_0544at 630.714 kb on - strand, within PP_0544at 630.714 kb on - strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.719 kb on + strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strandat 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.720 kb on - strand, within PP_0544at 630.800 kb on + strand, within PP_0544at 630.801 kb on - strand, within PP_0544at 631.013 kb on + strand, within PP_0544at 631.130 kb on - strand, within PP_0544at 631.232 kb on + strand, within PP_0544at 631.233 kb on - strandat 631.244 kb on + strand, within PP_0544at 631.244 kb on + strand, within PP_0544at 631.244 kb on + strand, within PP_0544at 631.244 kb on + strand, within PP_0544at 631.245 kb on - strand, within PP_0544at 631.394 kb on + strand, within PP_0544at 631.457 kb on + strand, within PP_0544at 631.457 kb on + strandat 631.458 kb on - strand, within PP_0544at 631.458 kb on - strand, within PP_0544at 631.535 kb on + strand, within PP_0544at 631.554 kb on - strandat 631.554 kb on - strandat 631.580 kb on - strandat 631.623 kb on - strandat 631.692 kb on + strandat 631.692 kb on + strandat 631.850 kb on + strandat 631.850 kb on + strandat 631.851 kb on - strandat 631.884 kb on + strandat 631.922 kb on - strandat 631.949 kb on - strandat 631.949 kb on - strandat 631.978 kb on + strandat 631.978 kb on + strandat 632.057 kb on - strandat 632.065 kb on + strandat 632.066 kb on - strandat 632.066 kb on - strandat 632.290 kb on + strand, within PP_0545at 632.290 kb on + strand, within PP_0545at 632.290 kb on + strand, within PP_0545at 632.290 kb on + strand, within PP_0545at 632.291 kb on - strand, within PP_0545at 632.378 kb on - strand, within PP_0545at 632.378 kb on - strand, within PP_0545at 632.378 kb on - strand, within PP_0545

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Per-strain Table

Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
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629,257 + PP_0543 0.33 +1.0
629,258 - PP_0543 0.33 -0.2
629,258 - PP_0543 0.33 +0.7
629,338 + PP_0543 0.38 +1.0
629,389 + PP_0543 0.42 -0.5
629,389 + PP_0543 0.42 +0.7
629,389 + PP_0543 0.42 -1.4
629,390 - PP_0543 0.42 +1.4
629,549 - PP_0543 0.53 +0.7
629,648 - PP_0543 0.61 -0.5
629,648 - PP_0543 0.61 -0.8
630,197 + +0.1
630,323 + +1.1
630,324 - +0.8
630,324 - +0.3
630,335 + -0.4
630,336 - +0.2
630,336 - +0.6
630,336 - -0.7
630,343 + -0.2
630,344 - -0.5
630,348 - +0.1
630,353 + +0.5
630,353 + -0.2
630,353 + +0.5
630,353 + +0.5
630,354 - -1.1
630,467 + PP_0544 0.15 +0.4
630,530 + PP_0544 0.20 -1.5
630,530 + PP_0544 0.20 -0.2
630,530 + PP_0544 0.20 -1.0
630,531 - PP_0544 0.20 -1.2
630,531 - PP_0544 0.20 +1.2
630,531 - PP_0544 0.20 -0.0
630,714 - PP_0544 0.32 +0.8
630,714 - PP_0544 0.32 -0.0
630,714 - PP_0544 0.32 +0.7
630,719 + PP_0544 0.33 +0.8
630,719 + PP_0544 0.33 +0.9
630,719 + PP_0544 0.33 +0.6
630,719 + PP_0544 0.33 -0.8
630,719 + PP_0544 0.33 -2.5
630,719 + PP_0544 0.33 +0.4
630,719 + PP_0544 0.33 +0.7
630,719 + PP_0544 0.33 +0.2
630,719 + PP_0544 0.33 +0.0
630,719 + PP_0544 0.33 +0.3
630,719 + PP_0544 0.33 +0.0
630,719 + PP_0544 0.33 +0.1
630,720 - PP_0544 0.33 +1.6
630,720 - PP_0544 0.33 -0.1
630,720 - PP_0544 0.33 +0.9
630,720 - PP_0544 0.33 +1.3
630,720 - PP_0544 0.33 +1.0
630,720 - PP_0544 0.33 -1.6
630,720 - PP_0544 0.33 +0.9
630,720 - PP_0544 0.33 +0.3
630,720 - PP_0544 0.33 +0.1
630,720 - PP_0544 0.33 -0.0
630,720 - -1.8
630,720 - PP_0544 0.33 +0.5
630,720 - PP_0544 0.33 +0.2
630,720 - PP_0544 0.33 +0.6
630,800 + PP_0544 0.38 -0.8
630,801 - PP_0544 0.38 +1.1
631,013 + PP_0544 0.53 +0.9
631,130 - PP_0544 0.61 +0.6
631,232 + PP_0544 0.68 -0.8
631,233 - +0.5
631,244 + PP_0544 0.69 +1.3
631,244 + PP_0544 0.69 +0.2
631,244 + PP_0544 0.69 +0.1
631,244 + PP_0544 0.69 +0.6
631,245 - PP_0544 0.69 +0.9
631,394 + PP_0544 0.79 +0.8
631,457 + PP_0544 0.84 -1.0
631,457 + +1.1
631,458 - PP_0544 0.84 -0.6
631,458 - PP_0544 0.84 +0.1
631,535 + PP_0544 0.89 +0.5
631,554 - +0.1
631,554 - +0.1
631,580 - +0.0
631,623 - -1.6
631,692 + -2.4
631,692 + +0.6
631,850 + +2.2
631,850 + +0.5
631,851 - +1.0
631,884 + -1.7
631,922 - -0.1
631,949 - -0.2
631,949 - -0.2
631,978 + +0.1
631,978 + +0.7
632,057 - -0.2
632,065 + +0.5
632,066 - -1.3
632,066 - +0.1
632,290 + PP_0545 0.24 -0.4
632,290 + PP_0545 0.24 -0.3
632,290 + PP_0545 0.24 -0.6
632,290 + PP_0545 0.24 +0.1
632,291 - PP_0545 0.24 +0.3
632,378 - PP_0545 0.30 -1.0
632,378 - PP_0545 0.30 +1.0
632,378 - PP_0545 0.30 +0.3

Or see this region's nucleotide sequence