Strain Fitness in Pseudomonas putida KT2440 around PP_0368

Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0366 and PP_0367 are separated by 173 nucleotidesPP_0367 and PP_0368 are separated by 287 nucleotidesPP_0368 and PP_0369 are separated by 301 nucleotides PP_0366: PP_0366 - dethiobiotin synthetase, at 443,838 to 444,518 _0366 PP_0367: PP_0367 - conserved protein of unknown function, at 444,692 to 445,027 _0367 PP_0368: PP_0368 - putative Acyl-CoA dehydrogenase, at 445,315 to 447,120 _0368 PP_0369: PP_0369 - putative diguanylate cyclase, at 447,422 to 448,705 _0369 Position (kb) 445 446 447 448Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 444.517 kb on - strandat 444.517 kb on - strandat 444.517 kb on - strandat 444.517 kb on - strandat 444.559 kb on + strandat 444.594 kb on - strandat 444.630 kb on + strandat 444.631 kb on - strandat 444.659 kb on + strandat 444.659 kb on + strandat 444.679 kb on + strandat 444.690 kb on - strandat 444.758 kb on + strand, within PP_0367at 444.758 kb on + strand, within PP_0367at 444.758 kb on + strand, within PP_0367at 444.758 kb on + strand, within PP_0367at 444.758 kb on + strand, within PP_0367at 444.759 kb on - strand, within PP_0367at 444.759 kb on - strand, within PP_0367at 444.850 kb on + strand, within PP_0367at 445.019 kb on + strandat 445.019 kb on + strandat 445.019 kb on + strandat 445.020 kb on - strandat 445.020 kb on - strandat 445.020 kb on - strandat 445.030 kb on - strandat 445.030 kb on - strandat 445.045 kb on + strandat 445.045 kb on + strandat 445.046 kb on - strandat 445.064 kb on + strandat 445.065 kb on - strandat 445.065 kb on - strandat 445.065 kb on - strandat 445.145 kb on + strandat 445.145 kb on + strandat 445.206 kb on - strandat 445.239 kb on - strandat 445.300 kb on + strandat 445.325 kb on - strandat 445.375 kb on + strandat 445.387 kb on + strandat 445.551 kb on + strand, within PP_0368at 445.552 kb on - strand, within PP_0368at 445.689 kb on + strand, within PP_0368at 445.693 kb on + strand, within PP_0368at 445.725 kb on + strand, within PP_0368at 445.726 kb on - strand, within PP_0368at 445.762 kb on + strand, within PP_0368at 445.762 kb on + strand, within PP_0368at 445.763 kb on - strand, within PP_0368at 445.879 kb on + strand, within PP_0368at 445.879 kb on + strand, within PP_0368at 445.880 kb on - strand, within PP_0368at 445.947 kb on + strand, within PP_0368at 446.101 kb on - strand, within PP_0368at 446.106 kb on + strand, within PP_0368at 446.107 kb on - strand, within PP_0368at 446.140 kb on + strand, within PP_0368at 446.407 kb on + strand, within PP_0368at 446.408 kb on - strand, within PP_0368at 446.408 kb on - strand, within PP_0368at 446.408 kb on - strand, within PP_0368at 446.408 kb on - strand, within PP_0368at 446.440 kb on + strand, within PP_0368at 446.441 kb on - strand, within PP_0368at 446.553 kb on - strand, within PP_0368at 446.619 kb on + strand, within PP_0368at 446.619 kb on + strand, within PP_0368at 446.619 kb on + strand, within PP_0368at 446.620 kb on - strand, within PP_0368at 446.620 kb on - strand, within PP_0368at 446.620 kb on - strand, within PP_0368at 446.620 kb on - strand, within PP_0368at 446.620 kb on - strand, within PP_0368at 446.620 kb on - strand, within PP_0368at 446.632 kb on + strand, within PP_0368at 446.633 kb on - strand, within PP_0368at 446.881 kb on + strand, within PP_0368at 446.891 kb on - strand, within PP_0368at 446.891 kb on - strand, within PP_0368at 446.891 kb on - strandat 446.899 kb on + strand, within PP_0368at 446.899 kb on + strand, within PP_0368at 446.915 kb on - strand, within PP_0368at 446.915 kb on - strand, within PP_0368at 446.983 kb on + strandat 446.984 kb on - strandat 447.013 kb on + strandat 447.013 kb on + strandat 447.013 kb on + strandat 447.036 kb on + strandat 447.115 kb on + strandat 447.115 kb on + strandat 447.115 kb on + strandat 447.116 kb on - strandat 447.116 kb on - strandat 447.122 kb on - strandat 447.202 kb on - strandat 447.213 kb on + strandat 447.279 kb on + strandat 447.280 kb on - strandat 447.302 kb on - strandat 447.394 kb on + strandat 447.395 kb on - strandat 447.417 kb on + strandat 447.500 kb on + strandat 447.501 kb on - strandat 447.501 kb on - strandat 447.501 kb on - strandat 447.501 kb on - strandat 447.550 kb on + strandat 447.551 kb on - strand, within PP_0369at 447.579 kb on - strand, within PP_0369at 447.588 kb on + strand, within PP_0369at 447.589 kb on - strand, within PP_0369at 447.590 kb on + strand, within PP_0369at 447.590 kb on + strand, within PP_0369at 447.591 kb on - strandat 447.629 kb on + strand, within PP_0369at 447.629 kb on + strand, within PP_0369at 447.630 kb on - strand, within PP_0369at 447.630 kb on - strand, within PP_0369at 447.630 kb on - strand, within PP_0369at 447.638 kb on + strand, within PP_0369at 447.668 kb on + strand, within PP_0369at 447.669 kb on - strand, within PP_0369at 447.669 kb on - strand, within PP_0369at 447.753 kb on - strand, within PP_0369at 447.753 kb on - strand, within PP_0369at 447.772 kb on + strand, within PP_0369at 447.772 kb on + strand, within PP_0369at 447.773 kb on - strandat 447.773 kb on - strand, within PP_0369at 447.773 kb on - strand, within PP_0369at 447.773 kb on - strand, within PP_0369at 447.773 kb on - strand, within PP_0369at 447.938 kb on + strand, within PP_0369at 447.939 kb on - strand, within PP_0369at 447.980 kb on + strand, within PP_0369at 447.980 kb on + strand, within PP_0369at 447.980 kb on + strand, within PP_0369at 447.981 kb on - strand, within PP_0369at 447.981 kb on - strand, within PP_0369at 448.073 kb on - strand, within PP_0369

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Per-strain Table

Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
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444,517 - -0.2
444,517 - +0.5
444,517 - -0.4
444,517 - -0.2
444,559 + +0.2
444,594 - +0.9
444,630 + +0.2
444,631 - -0.0
444,659 + +0.6
444,659 + +0.0
444,679 + +0.7
444,690 - +0.3
444,758 + PP_0367 0.20 +0.2
444,758 + PP_0367 0.20 -0.9
444,758 + PP_0367 0.20 +0.4
444,758 + PP_0367 0.20 -0.6
444,758 + PP_0367 0.20 -0.3
444,759 - PP_0367 0.20 +0.2
444,759 - PP_0367 0.20 -0.2
444,850 + PP_0367 0.47 -0.1
445,019 + +0.2
445,019 + -0.5
445,019 + -0.0
445,020 - -0.4
445,020 - +0.3
445,020 - -0.4
445,030 - +0.3
445,030 - -1.6
445,045 + -0.1
445,045 + +0.8
445,046 - +0.3
445,064 + -1.2
445,065 - +0.4
445,065 - +0.6
445,065 - -2.1
445,145 + -0.6
445,145 + +0.0
445,206 - +0.8
445,239 - -0.5
445,300 + +0.1
445,325 - -1.4
445,375 + +0.6
445,387 + -1.2
445,551 + PP_0368 0.13 +0.4
445,552 - PP_0368 0.13 +0.4
445,689 + PP_0368 0.21 +0.2
445,693 + PP_0368 0.21 -1.1
445,725 + PP_0368 0.23 -1.8
445,726 - PP_0368 0.23 -0.4
445,762 + PP_0368 0.25 -1.4
445,762 + PP_0368 0.25 -1.5
445,763 - PP_0368 0.25 +1.4
445,879 + PP_0368 0.31 -0.7
445,879 + PP_0368 0.31 -2.4
445,880 - PP_0368 0.31 -0.6
445,947 + PP_0368 0.35 -1.0
446,101 - PP_0368 0.44 -2.8
446,106 + PP_0368 0.44 -1.7
446,107 - PP_0368 0.44 -0.8
446,140 + PP_0368 0.46 -1.0
446,407 + PP_0368 0.60 -0.6
446,408 - PP_0368 0.61 -0.2
446,408 - PP_0368 0.61 -0.4
446,408 - PP_0368 0.61 -1.8
446,408 - PP_0368 0.61 -0.9
446,440 + PP_0368 0.62 +0.6
446,441 - PP_0368 0.62 +0.5
446,553 - PP_0368 0.69 -0.5
446,619 + PP_0368 0.72 +0.1
446,619 + PP_0368 0.72 -0.7
446,619 + PP_0368 0.72 -0.5
446,620 - PP_0368 0.72 -0.8
446,620 - PP_0368 0.72 -1.9
446,620 - PP_0368 0.72 -1.1
446,620 - PP_0368 0.72 +0.2
446,620 - PP_0368 0.72 -0.4
446,620 - PP_0368 0.72 -1.1
446,632 + PP_0368 0.73 +0.6
446,633 - PP_0368 0.73 -0.1
446,881 + PP_0368 0.87 -1.1
446,891 - PP_0368 0.87 +0.0
446,891 - PP_0368 0.87 -0.6
446,891 - +2.2
446,899 + PP_0368 0.88 -2.3
446,899 + PP_0368 0.88 -0.9
446,915 - PP_0368 0.89 -0.9
446,915 - PP_0368 0.89 -1.7
446,983 + -0.4
446,984 - -2.7
447,013 + -0.4
447,013 + -1.2
447,013 + -0.4
447,036 + -1.0
447,115 + +2.4
447,115 + +0.0
447,115 + -1.0
447,116 - +1.3
447,116 - +0.5
447,122 - -0.1
447,202 - +0.8
447,213 + +1.5
447,279 + -0.1
447,280 - -0.4
447,302 - +0.2
447,394 + -0.1
447,395 - -0.5
447,417 + +0.5
447,500 + +0.0
447,501 - -0.4
447,501 - -0.4
447,501 - -0.8
447,501 - -1.4
447,550 + +0.4
447,551 - PP_0369 0.10 +1.1
447,579 - PP_0369 0.12 +0.2
447,588 + PP_0369 0.13 +0.1
447,589 - PP_0369 0.13 +0.5
447,590 + PP_0369 0.13 -0.1
447,590 + PP_0369 0.13 -0.0
447,591 - +2.2
447,629 + PP_0369 0.16 -0.5
447,629 + PP_0369 0.16 -0.8
447,630 - PP_0369 0.16 -0.5
447,630 - PP_0369 0.16 -0.5
447,630 - PP_0369 0.16 +0.2
447,638 + PP_0369 0.17 -1.1
447,668 + PP_0369 0.19 -0.5
447,669 - PP_0369 0.19 +2.8
447,669 - PP_0369 0.19 +0.2
447,753 - PP_0369 0.26 +0.7
447,753 - PP_0369 0.26 -1.9
447,772 + PP_0369 0.27 -0.3
447,772 + PP_0369 0.27 -0.4
447,773 - +1.8
447,773 - PP_0369 0.27 -0.1
447,773 - PP_0369 0.27 -0.3
447,773 - PP_0369 0.27 -0.5
447,773 - PP_0369 0.27 +0.5
447,938 + PP_0369 0.40 +1.4
447,939 - PP_0369 0.40 -1.1
447,980 + PP_0369 0.43 +0.0
447,980 + PP_0369 0.43 +0.6
447,980 + PP_0369 0.43 +0.3
447,981 - PP_0369 0.44 -0.0
447,981 - PP_0369 0.44 +0.7
448,073 - PP_0369 0.51 -1.0

Or see this region's nucleotide sequence