Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0365 and PP_0366 are separated by 10 nucleotides PP_0366 and PP_0367 are separated by 173 nucleotides PP_0367 and PP_0368 are separated by 287 nucleotides
PP_0365: PP_0365 - Malonyl-[acyl-carrier protein] O-methyltransferase, at 443,009 to 443,827
_0365
PP_0366: PP_0366 - dethiobiotin synthetase, at 443,838 to 444,518
_0366
PP_0367: PP_0367 - conserved protein of unknown function, at 444,692 to 445,027
_0367
PP_0368: PP_0368 - putative Acyl-CoA dehydrogenase, at 445,315 to 447,120
_0368
Position (kb) 444
445
446 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.786 kb on + strand at 443.786 kb on + strand at 443.786 kb on + strand at 443.787 kb on - strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.826 kb on - strand at 444.043 kb on - strand, within PP_0366 at 444.043 kb on - strand, within PP_0366 at 444.077 kb on + strand, within PP_0366 at 444.077 kb on + strand, within PP_0366 at 444.078 kb on - strand, within PP_0366 at 444.080 kb on - strand, within PP_0366 at 444.184 kb on + strand, within PP_0366 at 444.184 kb on + strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.517 kb on - strand at 444.517 kb on - strand at 444.517 kb on - strand at 444.517 kb on - strand at 444.559 kb on + strand at 444.594 kb on - strand at 444.630 kb on + strand at 444.631 kb on - strand at 444.659 kb on + strand at 444.659 kb on + strand at 444.679 kb on + strand at 444.690 kb on - strand at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.759 kb on - strand, within PP_0367 at 444.759 kb on - strand, within PP_0367 at 444.850 kb on + strand, within PP_0367 at 445.019 kb on + strand at 445.019 kb on + strand at 445.019 kb on + strand at 445.020 kb on - strand at 445.020 kb on - strand at 445.020 kb on - strand at 445.030 kb on - strand at 445.030 kb on - strand at 445.045 kb on + strand at 445.045 kb on + strand at 445.046 kb on - strand at 445.064 kb on + strand at 445.065 kb on - strand at 445.065 kb on - strand at 445.065 kb on - strand at 445.145 kb on + strand at 445.145 kb on + strand at 445.206 kb on - strand at 445.239 kb on - strand at 445.300 kb on + strand at 445.325 kb on - strand at 445.375 kb on + strand at 445.387 kb on + strand at 445.551 kb on + strand, within PP_0368 at 445.552 kb on - strand, within PP_0368 at 445.689 kb on + strand, within PP_0368 at 445.693 kb on + strand, within PP_0368 at 445.725 kb on + strand, within PP_0368 at 445.726 kb on - strand, within PP_0368 at 445.762 kb on + strand, within PP_0368 at 445.762 kb on + strand, within PP_0368 at 445.763 kb on - strand, within PP_0368 at 445.879 kb on + strand, within PP_0368 at 445.879 kb on + strand, within PP_0368 at 445.880 kb on - strand, within PP_0368 at 445.947 kb on + strand, within PP_0368
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 443,747 + +0.6 443,747 + -1.4 443,747 + -1.1 443,747 + -1.1 443,747 + -0.9 443,748 - -2.1 443,748 - -3.6 443,748 - -0.7 443,748 - -0.9 443,786 + -2.8 443,786 + +1.2 443,786 + -2.0 443,787 - -2.4 443,798 + -1.2 443,798 + -3.1 443,798 + -1.8 443,798 + -1.7 443,798 + -1.4 443,798 + -2.5 443,799 - -1.9 443,799 - -2.2 443,799 - -1.9 443,799 - -4.0 443,799 - -2.8 443,799 - -3.4 443,826 - -1.9 444,043 - PP_0366 0.30 -1.0 444,043 - PP_0366 0.30 -2.5 444,077 + PP_0366 0.35 -3.8 444,077 + PP_0366 0.35 -3.6 444,078 - PP_0366 0.35 +0.2 444,080 - PP_0366 0.36 -2.9 444,184 + PP_0366 0.51 -5.3 444,184 + PP_0366 0.51 -2.7 444,185 - PP_0366 0.51 -4.4 444,185 - PP_0366 0.51 -3.4 444,185 - PP_0366 0.51 -0.9 444,517 - -0.2 444,517 - +0.5 444,517 - -0.4 444,517 - -0.2 444,559 + +0.2 444,594 - +0.9 444,630 + +0.2 444,631 - -0.0 444,659 + +0.6 444,659 + +0.0 444,679 + +0.7 444,690 - +0.3 444,758 + PP_0367 0.20 +0.2 444,758 + PP_0367 0.20 -0.9 444,758 + PP_0367 0.20 +0.4 444,758 + PP_0367 0.20 -0.6 444,758 + PP_0367 0.20 -0.3 444,759 - PP_0367 0.20 +0.2 444,759 - PP_0367 0.20 -0.2 444,850 + PP_0367 0.47 -0.1 445,019 + +0.2 445,019 + -0.5 445,019 + -0.0 445,020 - -0.4 445,020 - +0.3 445,020 - -0.4 445,030 - +0.3 445,030 - -1.6 445,045 + -0.1 445,045 + +0.8 445,046 - +0.3 445,064 + -1.2 445,065 - +0.4 445,065 - +0.6 445,065 - -2.1 445,145 + -0.6 445,145 + +0.0 445,206 - +0.8 445,239 - -0.5 445,300 + +0.1 445,325 - -1.4 445,375 + +0.6 445,387 + -1.2 445,551 + PP_0368 0.13 +0.4 445,552 - PP_0368 0.13 +0.4 445,689 + PP_0368 0.21 +0.2 445,693 + PP_0368 0.21 -1.1 445,725 + PP_0368 0.23 -1.8 445,726 - PP_0368 0.23 -0.4 445,762 + PP_0368 0.25 -1.4 445,762 + PP_0368 0.25 -1.5 445,763 - PP_0368 0.25 +1.4 445,879 + PP_0368 0.31 -0.7 445,879 + PP_0368 0.31 -2.4 445,880 - PP_0368 0.31 -0.6 445,947 + PP_0368 0.35 -1.0
Or see this region's nucleotide sequence