Strain Fitness in Pseudomonas putida KT2440 around PP_0064

Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0063 and PP_0064 are separated by 97 nucleotidesPP_0064 and PP_0065 are separated by 11 nucleotides PP_0063: PP_0063 - putative Lipid A biosynthesis lauroyl acyltransferase, at 73,525 to 74,412 _0063 PP_0064: PP_0064 - conserved protein of unknown function, at 74,510 to 74,824 _0064 PP_0065: PP_0065 - NAD-binding component of TrK potassium transporter, at 74,836 to 76,209 _0065 Position (kb) 74 75Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 73.512 kb on - strandat 73.662 kb on - strand, within PP_0063at 73.746 kb on + strandat 73.747 kb on - strand, within PP_0063at 73.858 kb on - strand, within PP_0063at 73.915 kb on + strand, within PP_0063at 73.915 kb on + strand, within PP_0063at 73.915 kb on + strand, within PP_0063at 73.915 kb on + strand, within PP_0063at 73.916 kb on - strand, within PP_0063at 74.082 kb on + strand, within PP_0063at 74.195 kb on + strand, within PP_0063at 74.196 kb on - strand, within PP_0063at 74.275 kb on + strand, within PP_0063at 74.275 kb on + strand, within PP_0063at 74.275 kb on + strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.329 kb on + strandat 74.330 kb on - strandat 74.330 kb on - strandat 74.330 kb on - strandat 74.330 kb on - strandat 74.341 kb on + strandat 74.353 kb on + strandat 74.353 kb on + strandat 74.354 kb on - strandat 74.354 kb on - strandat 74.354 kb on - strandat 74.410 kb on + strandat 74.410 kb on + strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.431 kb on + strandat 74.431 kb on + strandat 74.431 kb on + strandat 74.431 kb on + strandat 74.431 kb on + strandat 74.497 kb on + strandat 74.497 kb on + strandat 74.498 kb on - strandat 74.646 kb on + strand, within PP_0064at 74.647 kb on - strand, within PP_0064at 74.647 kb on - strand, within PP_0064at 74.647 kb on - strand, within PP_0064at 74.654 kb on - strand, within PP_0064at 74.679 kb on + strand, within PP_0064at 74.680 kb on - strand, within PP_0064at 74.696 kb on + strand, within PP_0064at 74.985 kb on + strand, within PP_0065at 75.196 kb on + strand, within PP_0065at 75.196 kb on + strand, within PP_0065at 75.209 kb on + strand, within PP_0065at 75.431 kb on + strand, within PP_0065at 75.517 kb on - strand, within PP_0065at 75.728 kb on + strand, within PP_0065at 75.729 kb on - strand, within PP_0065at 75.729 kb on - strand, within PP_0065at 75.732 kb on - strandat 75.818 kb on + strand, within PP_0065at 75.818 kb on + strand, within PP_0065at 75.818 kb on + strand, within PP_0065at 75.818 kb on + strand, within PP_0065at 75.819 kb on - strand, within PP_0065

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Per-strain Table

Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
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73,512 - +1.3
73,662 - PP_0063 0.15 +0.3
73,746 + +2.3
73,747 - PP_0063 0.25 -1.4
73,858 - PP_0063 0.38 -0.5
73,915 + PP_0063 0.44 -0.5
73,915 + PP_0063 0.44 +0.7
73,915 + PP_0063 0.44 +0.1
73,915 + PP_0063 0.44 -0.1
73,916 - PP_0063 0.44 -0.5
74,082 + PP_0063 0.63 +1.3
74,195 + PP_0063 0.75 +0.3
74,196 - PP_0063 0.76 +1.3
74,275 + PP_0063 0.84 -0.9
74,275 + PP_0063 0.84 +0.8
74,275 + PP_0063 0.84 +1.0
74,276 - PP_0063 0.85 -0.0
74,276 - PP_0063 0.85 +2.3
74,276 - PP_0063 0.85 +1.3
74,276 - PP_0063 0.85 -1.9
74,276 - PP_0063 0.85 +0.8
74,329 + +0.3
74,330 - +2.3
74,330 - +0.3
74,330 - -1.9
74,330 - +1.3
74,341 + +0.6
74,353 + +2.3
74,353 + +2.3
74,354 - +1.3
74,354 - +1.3
74,354 - -1.3
74,410 + -0.7
74,410 + -1.8
74,411 - -0.7
74,411 - -0.5
74,411 - +0.4
74,411 - -0.2
74,411 - +0.6
74,411 - -0.3
74,431 + -1.2
74,431 + -0.7
74,431 + +0.4
74,431 + -1.6
74,431 + -0.2
74,497 + -0.1
74,497 + +0.2
74,498 - -0.4
74,646 + PP_0064 0.43 -1.5
74,647 - PP_0064 0.43 -0.8
74,647 - PP_0064 0.43 -0.6
74,647 - PP_0064 0.43 -0.1
74,654 - PP_0064 0.46 -1.1
74,679 + PP_0064 0.54 -0.3
74,680 - PP_0064 0.54 +0.3
74,696 + PP_0064 0.59 -0.0
74,985 + PP_0065 0.11 +0.5
75,196 + PP_0065 0.26 -3.0
75,196 + PP_0065 0.26 -0.0
75,209 + PP_0065 0.27 +0.7
75,431 + PP_0065 0.43 -0.3
75,517 - PP_0065 0.50 -1.9
75,728 + PP_0065 0.65 -0.6
75,729 - PP_0065 0.65 +0.7
75,729 - PP_0065 0.65 +0.7
75,732 - -0.3
75,818 + PP_0065 0.71 -1.0
75,818 + PP_0065 0.71 +0.7
75,818 + PP_0065 0.71 +1.3
75,818 + PP_0065 0.71 -1.2
75,819 - PP_0065 0.72 +0.3

Or see this region's nucleotide sequence