Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4448 and PP_5681 are separated by 267 nucleotides PP_5681 and PP_4449 are separated by 180 nucleotides PP_4449 and PP_5682 are separated by 92 nucleotides PP_5682 and PP_4450 are separated by 107 nucleotides
PP_4448: PP_4448 - conserved protein of unknown function, at 5,042,931 to 5,044,694
_4448
PP_5681: PP_5681 - conserved protein of unknown function, at 5,044,962 to 5,045,198
_5681
PP_4449: PP_4449 - conserved protein of unknown function, at 5,045,379 to 5,045,633
_4449
PP_5682: PP_5682 - conserved protein of unknown function, at 5,045,726 to 5,045,935
_5682
PP_4450: PP_4450 - Phosphoglycerate mutase family protein, at 5,046,043 to 5,046,669
_4450
Position (kb)
5044
5045
5046 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5044.074 kb on + strand, within PP_4448 at 5044.151 kb on - strand, within PP_4448 at 5044.185 kb on + strand, within PP_4448 at 5044.191 kb on + strand, within PP_4448 at 5044.249 kb on + strand, within PP_4448 at 5044.249 kb on + strand, within PP_4448 at 5044.273 kb on - strand, within PP_4448 at 5044.273 kb on - strand, within PP_4448 at 5044.464 kb on + strand, within PP_4448 at 5044.464 kb on + strand, within PP_4448 at 5044.465 kb on - strand, within PP_4448 at 5044.688 kb on + strand at 5044.688 kb on + strand at 5044.689 kb on - strand at 5044.689 kb on - strand at 5044.692 kb on + strand at 5044.692 kb on + strand at 5044.692 kb on + strand at 5044.693 kb on - strand at 5044.743 kb on - strand at 5044.743 kb on - strand at 5044.743 kb on - strand at 5044.743 kb on - strand at 5044.808 kb on + strand at 5044.809 kb on - strand at 5044.819 kb on + strand at 5044.819 kb on + strand at 5044.819 kb on + strand at 5044.866 kb on + strand at 5044.867 kb on - strand at 5044.930 kb on - strand at 5044.934 kb on + strand at 5044.934 kb on + strand at 5044.934 kb on + strand at 5044.934 kb on + strand at 5044.935 kb on - strand at 5045.042 kb on + strand, within PP_5681 at 5045.043 kb on - strand, within PP_5681 at 5045.044 kb on + strand, within PP_5681 at 5045.045 kb on - strand, within PP_5681 at 5045.045 kb on - strand, within PP_5681 at 5045.045 kb on - strand, within PP_5681 at 5045.045 kb on - strand, within PP_5681 at 5045.130 kb on - strand, within PP_5681 at 5045.259 kb on + strand at 5045.293 kb on + strand at 5045.298 kb on + strand at 5045.312 kb on - strand at 5045.312 kb on - strand at 5045.325 kb on - strand at 5045.329 kb on + strand at 5045.330 kb on - strand at 5045.381 kb on - strand at 5045.393 kb on + strand at 5045.393 kb on + strand at 5045.393 kb on + strand at 5045.475 kb on + strand, within PP_4449 at 5045.476 kb on - strand, within PP_4449 at 5045.530 kb on + strand, within PP_4449 at 5045.531 kb on - strand, within PP_4449 at 5045.570 kb on + strand, within PP_4449 at 5045.570 kb on + strand, within PP_4449 at 5045.625 kb on + strand at 5045.625 kb on + strand at 5045.626 kb on - strand at 5045.626 kb on - strand at 5045.702 kb on + strand at 5045.727 kb on + strand at 5045.728 kb on - strand at 5045.728 kb on - strand at 5045.735 kb on + strand at 5045.735 kb on + strand at 5045.736 kb on - strand at 5045.761 kb on + strand, within PP_5682 at 5045.776 kb on + strand, within PP_5682 at 5045.834 kb on + strand, within PP_5682 at 5045.835 kb on - strand, within PP_5682 at 5045.835 kb on - strand, within PP_5682 at 5045.860 kb on - strand, within PP_5682 at 5045.866 kb on + strand, within PP_5682 at 5045.866 kb on + strand, within PP_5682 at 5045.867 kb on - strand, within PP_5682 at 5045.867 kb on - strand, within PP_5682 at 5045.867 kb on - strand, within PP_5682 at 5045.889 kb on + strand, within PP_5682 at 5045.890 kb on - strand, within PP_5682 at 5045.890 kb on - strand, within PP_5682 at 5045.890 kb on - strand, within PP_5682 at 5045.910 kb on - strand, within PP_5682 at 5045.996 kb on + strand at 5045.997 kb on - strand at 5046.004 kb on - strand at 5046.018 kb on + strand at 5046.019 kb on - strand at 5046.019 kb on - strand at 5046.019 kb on - strand at 5046.019 kb on - strand at 5046.019 kb on - strand at 5046.038 kb on - strand at 5046.053 kb on + strand at 5046.053 kb on + strand at 5046.054 kb on - strand at 5046.175 kb on - strand, within PP_4450
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 5,044,074 + PP_4448 0.65 +1.1 5,044,151 - PP_4448 0.69 -0.3 5,044,185 + PP_4448 0.71 -0.8 5,044,191 + PP_4448 0.71 +0.5 5,044,249 + PP_4448 0.75 +0.0 5,044,249 + PP_4448 0.75 +0.1 5,044,273 - PP_4448 0.76 -0.2 5,044,273 - PP_4448 0.76 -1.6 5,044,464 + PP_4448 0.87 +0.9 5,044,464 + PP_4448 0.87 +0.2 5,044,465 - PP_4448 0.87 -2.2 5,044,688 + -0.1 5,044,688 + +0.2 5,044,689 - -0.5 5,044,689 - +0.2 5,044,692 + +0.2 5,044,692 + +1.3 5,044,692 + +0.2 5,044,693 - -2.2 5,044,743 - +0.1 5,044,743 - +0.5 5,044,743 - +0.2 5,044,743 - -0.2 5,044,808 + +1.9 5,044,809 - -1.9 5,044,819 + -0.2 5,044,819 + +0.9 5,044,819 + +0.3 5,044,866 + +0.3 5,044,867 - +0.9 5,044,930 - -0.1 5,044,934 + +1.9 5,044,934 + -1.2 5,044,934 + -0.8 5,044,934 + -0.4 5,044,935 - -1.2 5,045,042 + PP_5681 0.34 +0.4 5,045,043 - PP_5681 0.34 +0.3 5,045,044 + PP_5681 0.35 +0.9 5,045,045 - PP_5681 0.35 +0.6 5,045,045 - PP_5681 0.35 +0.4 5,045,045 - PP_5681 0.35 +0.1 5,045,045 - PP_5681 0.35 +0.5 5,045,130 - PP_5681 0.71 +0.0 5,045,259 + +0.4 5,045,293 + +1.0 5,045,298 + +0.1 5,045,312 - +1.5 5,045,312 - -0.2 5,045,325 - -1.3 5,045,329 + -0.3 5,045,330 - +0.2 5,045,381 - -0.2 5,045,393 + +1.7 5,045,393 + -0.7 5,045,393 + +1.6 5,045,475 + PP_4449 0.38 +0.3 5,045,476 - PP_4449 0.38 -1.2 5,045,530 + PP_4449 0.59 -0.4 5,045,531 - PP_4449 0.60 +0.2 5,045,570 + PP_4449 0.75 +0.5 5,045,570 + PP_4449 0.75 -0.2 5,045,625 + +0.2 5,045,625 + -0.2 5,045,626 - -0.0 5,045,626 - +3.5 5,045,702 + +0.8 5,045,727 + -0.6 5,045,728 - +0.4 5,045,728 - -0.3 5,045,735 + -0.5 5,045,735 + +1.9 5,045,736 - +0.7 5,045,761 + PP_5682 0.17 -0.1 5,045,776 + PP_5682 0.24 +0.6 5,045,834 + PP_5682 0.51 -0.1 5,045,835 - PP_5682 0.52 +0.4 5,045,835 - PP_5682 0.52 +1.4 5,045,860 - PP_5682 0.64 -0.2 5,045,866 + PP_5682 0.67 +0.2 5,045,866 + PP_5682 0.67 -0.4 5,045,867 - PP_5682 0.67 +0.1 5,045,867 - PP_5682 0.67 -0.6 5,045,867 - PP_5682 0.67 -0.9 5,045,889 + PP_5682 0.78 -0.3 5,045,890 - PP_5682 0.78 +0.7 5,045,890 - PP_5682 0.78 -0.9 5,045,890 - PP_5682 0.78 -0.4 5,045,910 - PP_5682 0.88 +1.9 5,045,996 + +0.0 5,045,997 - -0.1 5,046,004 - -0.2 5,046,018 + -0.4 5,046,019 - +0.1 5,046,019 - +0.3 5,046,019 - -0.5 5,046,019 - +0.1 5,046,019 - -0.1 5,046,038 - +0.7 5,046,053 + -0.7 5,046,053 + -1.2 5,046,054 - +1.1 5,046,175 - PP_4450 0.21 -1.9
Or see this region's nucleotide sequence