Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1920 and PP_5483 are separated by 67 nucleotides PP_5483 and PP_1921 are separated by 780 nucleotides
PP_1920: PP_1920 - conserved protein of unknown function, at 2,164,317 to 2,165,537
_1920
PP_5483: PP_5483 - conserved protein of unknown function, at 2,165,605 to 2,166,156
_5483
PP_1921: PP_1921 - conserved protein of unknown function, at 2,166,937 to 2,168,100
_1921
Position (kb)
2165
2166
2167 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2164.632 kb on + strand, within PP_1920 at 2164.723 kb on - strand, within PP_1920 at 2164.738 kb on + strand, within PP_1920 at 2164.738 kb on + strand, within PP_1920 at 2164.739 kb on - strand, within PP_1920 at 2164.739 kb on - strand, within PP_1920 at 2164.739 kb on - strand, within PP_1920 at 2164.852 kb on + strand, within PP_1920 at 2164.931 kb on + strand, within PP_1920 at 2165.143 kb on - strand, within PP_1920 at 2165.232 kb on + strand, within PP_1920 at 2165.232 kb on - strand, within PP_1920 at 2165.266 kb on + strand, within PP_1920 at 2165.344 kb on - strand, within PP_1920 at 2165.348 kb on + strand, within PP_1920 at 2165.351 kb on - strand, within PP_1920 at 2165.351 kb on - strand, within PP_1920 at 2165.595 kb on - strand at 2165.785 kb on - strand, within PP_5483 at 2165.919 kb on + strand, within PP_5483 at 2166.133 kb on + strand at 2166.133 kb on + strand at 2166.158 kb on + strand at 2166.158 kb on + strand at 2166.159 kb on - strand at 2166.159 kb on - strand at 2166.182 kb on + strand at 2166.190 kb on + strand at 2166.191 kb on - strand at 2166.250 kb on + strand at 2166.250 kb on + strand at 2166.278 kb on + strand at 2166.278 kb on + strand at 2166.287 kb on + strand at 2166.288 kb on - strand at 2166.295 kb on + strand at 2166.296 kb on - strand at 2166.314 kb on - strand at 2166.319 kb on - strand at 2166.319 kb on - strand at 2166.380 kb on + strand at 2166.406 kb on + strand at 2166.406 kb on + strand at 2166.457 kb on - strand at 2166.490 kb on - strand at 2166.527 kb on + strand at 2166.528 kb on - strand at 2166.591 kb on - strand at 2166.604 kb on + strand at 2166.605 kb on - strand at 2166.624 kb on + strand at 2166.625 kb on - strand at 2166.625 kb on - strand at 2166.726 kb on - strand at 2166.733 kb on - strand at 2166.739 kb on + strand at 2166.739 kb on + strand at 2166.739 kb on + strand at 2166.746 kb on + strand at 2166.746 kb on + strand at 2166.747 kb on - strand at 2166.747 kb on - strand at 2166.821 kb on + strand at 2166.832 kb on + strand at 2166.832 kb on + strand at 2166.832 kb on + strand at 2166.832 kb on + strand at 2166.833 kb on - strand at 2166.833 kb on - strand at 2166.838 kb on + strand at 2166.942 kb on + strand at 2166.942 kb on + strand at 2166.943 kb on - strand at 2166.943 kb on - strand at 2166.946 kb on + strand at 2167.005 kb on - strand at 2167.041 kb on + strand at 2167.042 kb on - strand at 2167.042 kb on - strand at 2167.049 kb on - strand at 2167.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 2,164,632 + PP_1920 0.26 -0.3 2,164,723 - PP_1920 0.33 -1.9 2,164,738 + PP_1920 0.34 -0.3 2,164,738 + PP_1920 0.34 -0.8 2,164,739 - PP_1920 0.35 -0.1 2,164,739 - PP_1920 0.35 +0.2 2,164,739 - PP_1920 0.35 +0.7 2,164,852 + PP_1920 0.44 -0.4 2,164,931 + PP_1920 0.50 +0.1 2,165,143 - PP_1920 0.68 +0.6 2,165,232 + PP_1920 0.75 -1.3 2,165,232 - PP_1920 0.75 -0.3 2,165,266 + PP_1920 0.78 +0.1 2,165,344 - PP_1920 0.84 -0.7 2,165,348 + PP_1920 0.84 -0.4 2,165,351 - PP_1920 0.85 +1.6 2,165,351 - PP_1920 0.85 -0.0 2,165,595 - -1.6 2,165,785 - PP_5483 0.33 +1.1 2,165,919 + PP_5483 0.57 +0.8 2,166,133 + +0.5 2,166,133 + +0.1 2,166,158 + +0.5 2,166,158 + -1.0 2,166,159 - +0.3 2,166,159 - +0.9 2,166,182 + -1.6 2,166,190 + +0.3 2,166,191 - +0.0 2,166,250 + +2.6 2,166,250 + +1.1 2,166,278 + -0.0 2,166,278 + -0.7 2,166,287 + -1.0 2,166,288 - +0.5 2,166,295 + -0.4 2,166,296 - +0.5 2,166,314 - -1.8 2,166,319 - +0.3 2,166,319 - -0.9 2,166,380 + -0.4 2,166,406 + +0.1 2,166,406 + +0.1 2,166,457 - -0.1 2,166,490 - -0.4 2,166,527 + -0.8 2,166,528 - +0.2 2,166,591 - -1.3 2,166,604 + -0.2 2,166,605 - -0.9 2,166,624 + -0.8 2,166,625 - +1.2 2,166,625 - -0.4 2,166,726 - +0.2 2,166,733 - -0.1 2,166,739 + +0.6 2,166,739 + +0.9 2,166,739 + +0.2 2,166,746 + +0.0 2,166,746 + -0.3 2,166,747 - +0.2 2,166,747 - +0.9 2,166,821 + +0.8 2,166,832 + -1.6 2,166,832 + +0.3 2,166,832 + +0.2 2,166,832 + +0.3 2,166,833 - +1.3 2,166,833 - +0.9 2,166,838 + -2.0 2,166,942 + +0.1 2,166,942 + +0.6 2,166,943 - -0.7 2,166,943 - -1.0 2,166,946 + -0.3 2,167,005 - -0.9 2,167,041 + -0.0 2,167,042 - -0.1 2,167,042 - -0.4 2,167,049 - -0.3 2,167,049 - +0.2
Or see this region's nucleotide sequence