Strain Fitness in Pseudomonas putida KT2440 around PP_5441

Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0297 and PP_5441 are separated by 129 nucleotidesPP_5441 and PP_0298 are separated by 171 nucleotides PP_0297: PP_0297 - L-serine dehydratase, at 356,656 to 358,032 _0297 PP_5441: PP_5441 - protein of unknown function, at 358,162 to 358,362 _5441 PP_0298: PP_0298 - AraC-family transcriptional regulator, at 358,534 to 359,640 _0298 Position (kb) 358 359Strain fitness (log2 ratio) -2 -1 0 1 2at 357.337 kb on + strand, within PP_0297at 357.337 kb on + strandat 357.338 kb on - strand, within PP_0297at 357.338 kb on - strand, within PP_0297at 357.338 kb on - strand, within PP_0297at 357.562 kb on + strand, within PP_0297at 357.562 kb on + strand, within PP_0297at 357.562 kb on + strand, within PP_0297at 357.562 kb on + strand, within PP_0297at 357.562 kb on + strand, within PP_0297at 357.563 kb on - strand, within PP_0297at 357.563 kb on - strand, within PP_0297at 357.563 kb on - strand, within PP_0297at 357.565 kb on + strand, within PP_0297at 357.565 kb on + strand, within PP_0297at 357.565 kb on + strand, within PP_0297at 357.566 kb on - strand, within PP_0297at 357.566 kb on - strand, within PP_0297at 357.566 kb on - strand, within PP_0297at 357.610 kb on + strand, within PP_0297at 357.938 kb on + strandat 357.969 kb on + strandat 357.970 kb on - strandat 357.982 kb on + strandat 357.982 kb on + strandat 357.982 kb on + strandat 357.983 kb on - strandat 358.079 kb on + strandat 358.080 kb on - strandat 358.102 kb on - strandat 358.102 kb on - strandat 358.161 kb on + strandat 358.161 kb on + strandat 358.162 kb on - strandat 358.162 kb on - strandat 358.162 kb on - strandat 358.162 kb on - strandat 358.172 kb on - strandat 358.172 kb on - strandat 358.245 kb on + strand, within PP_5441at 358.245 kb on + strand, within PP_5441at 358.245 kb on + strand, within PP_5441at 358.246 kb on - strand, within PP_5441at 358.287 kb on - strand, within PP_5441at 358.287 kb on - strand, within PP_5441at 358.373 kb on - strandat 358.448 kb on + strandat 358.449 kb on - strandat 358.475 kb on + strandat 358.476 kb on - strandat 358.485 kb on + strandat 358.485 kb on + strandat 358.485 kb on + strandat 358.485 kb on + strandat 358.485 kb on + strandat 358.486 kb on - strandat 358.601 kb on + strandat 358.601 kb on + strandat 358.601 kb on + strandat 358.601 kb on + strandat 358.602 kb on - strandat 358.602 kb on - strandat 359.145 kb on + strand, within PP_0298at 359.281 kb on + strand, within PP_0298at 359.282 kb on - strand, within PP_0298at 359.282 kb on - strand, within PP_0298at 359.282 kb on - strand, within PP_0298at 359.282 kb on - strand, within PP_0298at 359.282 kb on - strand, within PP_0298at 359.282 kb on - strand, within PP_0298at 359.282 kb on - strand, within PP_0298at 359.350 kb on + strand, within PP_0298at 359.351 kb on - strand, within PP_0298at 359.353 kb on + strand, within PP_0298at 359.354 kb on - strand, within PP_0298at 359.354 kb on - strand, within PP_0298at 359.354 kb on - strand, within PP_0298

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Per-strain Table

Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
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357,337 + PP_0297 0.49 -1.5
357,337 + +2.3
357,338 - PP_0297 0.50 +0.5
357,338 - PP_0297 0.50 -0.1
357,338 - PP_0297 0.50 -0.9
357,562 + PP_0297 0.66 +1.6
357,562 + PP_0297 0.66 -1.4
357,562 + PP_0297 0.66 +0.1
357,562 + PP_0297 0.66 -1.6
357,562 + PP_0297 0.66 +0.3
357,563 - PP_0297 0.66 -0.9
357,563 - PP_0297 0.66 -0.6
357,563 - PP_0297 0.66 +0.4
357,565 + PP_0297 0.66 +0.3
357,565 + PP_0297 0.66 -0.3
357,565 + PP_0297 0.66 +0.4
357,566 - PP_0297 0.66 +0.7
357,566 - PP_0297 0.66 -0.9
357,566 - PP_0297 0.66 +0.1
357,610 + PP_0297 0.69 +0.3
357,938 + +0.7
357,969 + +2.0
357,970 - +0.5
357,982 + -0.3
357,982 + -0.7
357,982 + -0.8
357,983 - -0.4
358,079 + -0.3
358,080 - -0.3
358,102 - -0.3
358,102 - +0.0
358,161 + +0.1
358,161 + -1.3
358,162 - +0.1
358,162 - -0.7
358,162 - -1.4
358,162 - +0.2
358,172 - +0.1
358,172 - +0.0
358,245 + PP_5441 0.41 +1.3
358,245 + PP_5441 0.41 +2.3
358,245 + PP_5441 0.41 +0.4
358,246 - PP_5441 0.42 -0.8
358,287 - PP_5441 0.62 +1.4
358,287 - PP_5441 0.62 +1.3
358,373 - -0.1
358,448 + -0.1
358,449 - -1.2
358,475 + +1.3
358,476 - -2.0
358,485 + -1.2
358,485 + -0.0
358,485 + -0.4
358,485 + +2.3
358,485 + -1.3
358,486 - +1.0
358,601 + +0.1
358,601 + +0.1
358,601 + +0.6
358,601 + +0.5
358,602 - -0.6
358,602 - +0.5
359,145 + PP_0298 0.55 -0.5
359,281 + PP_0298 0.67 -1.7
359,282 - PP_0298 0.68 +1.1
359,282 - PP_0298 0.68 +0.6
359,282 - PP_0298 0.68 +1.1
359,282 - PP_0298 0.68 -0.6
359,282 - PP_0298 0.68 -0.6
359,282 - PP_0298 0.68 +0.2
359,282 - PP_0298 0.68 +0.6
359,350 + PP_0298 0.74 +1.3
359,351 - PP_0298 0.74 +0.7
359,353 + PP_0298 0.74 -0.0
359,354 - PP_0298 0.74 +0.7
359,354 - PP_0298 0.74 +0.4
359,354 - PP_0298 0.74 +0.2

Or see this region's nucleotide sequence