Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5335 and PP_5336 are separated by 10 nucleotides PP_5336 and PP_5337 are separated by 267 nucleotides PP_5337 and PP_5338 are separated by 170 nucleotides
PP_5335: PP_5335 - N5-carboxyaminoimidazole ribonucleotide synthase, at 6,081,951 to 6,083,033
_5335
PP_5336: PP_5336 - N5-carboxyaminoimidazole ribonucleotide mutase, at 6,083,044 to 6,083,535
_5336
PP_5337: PP_5337 - LysR family transcriptional regulator, at 6,083,803 to 6,084,720
_5337
PP_5338: PP_5338 - aspartate ammonia-lyase, at 6,084,891 to 6,086,327
_5338
Position (kb)
6083
6084
6085 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6083.662 kb on - strand at 6083.666 kb on - strand at 6083.666 kb on - strand at 6083.686 kb on + strand at 6083.686 kb on + strand at 6083.687 kb on - strand at 6083.788 kb on + strand at 6083.789 kb on - strand at 6083.789 kb on - strand at 6083.789 kb on - strand at 6083.851 kb on + strand at 6083.852 kb on - strand at 6083.865 kb on + strand at 6083.866 kb on - strand at 6083.891 kb on + strand at 6084.047 kb on + strand, within PP_5337 at 6084.058 kb on - strand, within PP_5337 at 6084.058 kb on - strand, within PP_5337 at 6084.059 kb on + strand, within PP_5337 at 6084.060 kb on - strand, within PP_5337 at 6084.123 kb on - strand, within PP_5337 at 6084.123 kb on - strand, within PP_5337 at 6084.123 kb on - strand, within PP_5337 at 6084.123 kb on - strand, within PP_5337 at 6084.123 kb on - strand, within PP_5337 at 6084.128 kb on - strand, within PP_5337 at 6084.272 kb on + strand, within PP_5337 at 6084.273 kb on - strand, within PP_5337 at 6084.316 kb on + strand, within PP_5337 at 6084.316 kb on + strand, within PP_5337 at 6084.316 kb on + strand, within PP_5337 at 6084.316 kb on + strand, within PP_5337 at 6084.316 kb on + strand, within PP_5337 at 6084.317 kb on - strand, within PP_5337 at 6084.317 kb on - strand, within PP_5337 at 6084.317 kb on - strand, within PP_5337 at 6084.334 kb on - strand, within PP_5337 at 6084.789 kb on + strand at 6084.805 kb on - strand at 6084.895 kb on + strand at 6084.896 kb on - strand at 6084.896 kb on - strand at 6084.897 kb on + strand at 6084.898 kb on - strand at 6084.898 kb on - strand at 6084.898 kb on - strand at 6084.898 kb on - strand at 6084.972 kb on + strand at 6084.972 kb on + strand at 6084.972 kb on + strand at 6084.972 kb on + strand at 6084.975 kb on + strand at 6084.975 kb on + strand at 6084.975 kb on + strand at 6085.214 kb on + strand, within PP_5338 at 6085.220 kb on + strand, within PP_5338 at 6085.220 kb on + strand, within PP_5338 at 6085.220 kb on + strand, within PP_5338 at 6085.221 kb on - strand, within PP_5338 at 6085.307 kb on + strand, within PP_5338 at 6085.308 kb on - strand, within PP_5338 at 6085.409 kb on + strand, within PP_5338 at 6085.456 kb on + strand, within PP_5338
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 6,083,662 - -3.3 6,083,666 - +0.7 6,083,666 - -2.2 6,083,686 + -0.4 6,083,686 + -0.4 6,083,687 - +1.6 6,083,788 + -0.4 6,083,789 - +0.2 6,083,789 - -0.3 6,083,789 - +0.0 6,083,851 + -0.4 6,083,852 - +0.3 6,083,865 + +1.3 6,083,866 - +0.6 6,083,891 + +2.3 6,084,047 + PP_5337 0.27 +0.5 6,084,058 - PP_5337 0.28 -1.3 6,084,058 - PP_5337 0.28 -0.3 6,084,059 + PP_5337 0.28 +0.8 6,084,060 - PP_5337 0.28 +0.7 6,084,123 - PP_5337 0.35 +1.4 6,084,123 - PP_5337 0.35 -0.5 6,084,123 - PP_5337 0.35 +1.4 6,084,123 - PP_5337 0.35 -0.7 6,084,123 - PP_5337 0.35 +2.3 6,084,128 - PP_5337 0.35 +0.3 6,084,272 + PP_5337 0.51 +0.7 6,084,273 - PP_5337 0.51 +2.3 6,084,316 + PP_5337 0.56 -0.7 6,084,316 + PP_5337 0.56 -0.3 6,084,316 + PP_5337 0.56 +1.0 6,084,316 + PP_5337 0.56 +2.3 6,084,316 + PP_5337 0.56 +0.6 6,084,317 - PP_5337 0.56 +0.2 6,084,317 - PP_5337 0.56 +0.3 6,084,317 - PP_5337 0.56 +0.5 6,084,334 - PP_5337 0.58 +2.8 6,084,789 + +0.9 6,084,805 - +0.4 6,084,895 + +2.3 6,084,896 - +2.3 6,084,896 - +0.2 6,084,897 + +0.7 6,084,898 - +2.3 6,084,898 - +0.7 6,084,898 - +0.2 6,084,898 - +0.3 6,084,972 + -0.3 6,084,972 + -0.3 6,084,972 + +0.0 6,084,972 + +1.3 6,084,975 + -0.5 6,084,975 + +2.3 6,084,975 + -0.3 6,085,214 + PP_5338 0.22 +0.7 6,085,220 + PP_5338 0.23 -0.3 6,085,220 + PP_5338 0.23 +0.0 6,085,220 + PP_5338 0.23 +1.0 6,085,221 - PP_5338 0.23 -1.0 6,085,307 + PP_5338 0.29 -0.3 6,085,308 - PP_5338 0.29 +2.3 6,085,409 + PP_5338 0.36 +0.7 6,085,456 + PP_5338 0.39 +0.3
Or see this region's nucleotide sequence