Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4944 and PP_4945 are separated by 192 nucleotides PP_4945 and PP_4946 are separated by 106 nucleotides
PP_4944: PP_4944 - Carbamoyltransferase, at 5,625,744 to 5,627,501
_4944
PP_4945: PP_4945 - 23S rRNA m(6)A2030 methyltransferase, at 5,627,694 to 5,628,530
_4945
PP_4946: PP_4946 - sodium/L-proline transporter, at 5,628,637 to 5,630,265
_4946
Position (kb) 5627
5628
5629 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5626.730 kb on - strand, within PP_4944 at 5626.730 kb on - strand, within PP_4944 at 5626.730 kb on - strand, within PP_4944 at 5626.928 kb on - strand, within PP_4944 at 5627.075 kb on - strand, within PP_4944 at 5627.252 kb on - strand, within PP_4944 at 5627.508 kb on - strand at 5627.574 kb on - strand at 5627.608 kb on + strand at 5627.609 kb on - strand at 5627.658 kb on + strand at 5627.695 kb on + strand at 5627.726 kb on + strand at 5627.860 kb on + strand, within PP_4945 at 5627.860 kb on + strand, within PP_4945 at 5627.860 kb on + strand, within PP_4945 at 5627.860 kb on + strand, within PP_4945 at 5627.861 kb on - strand, within PP_4945 at 5627.861 kb on - strand, within PP_4945 at 5627.861 kb on - strand, within PP_4945 at 5627.861 kb on - strand, within PP_4945 at 5627.861 kb on - strand, within PP_4945 at 5627.912 kb on - strand, within PP_4945 at 5627.912 kb on - strand, within PP_4945 at 5627.915 kb on - strand, within PP_4945 at 5628.185 kb on + strand, within PP_4945 at 5628.247 kb on + strand, within PP_4945 at 5628.248 kb on - strand, within PP_4945 at 5628.292 kb on + strand, within PP_4945 at 5628.293 kb on - strand, within PP_4945 at 5628.391 kb on + strand, within PP_4945 at 5628.391 kb on + strand, within PP_4945 at 5628.391 kb on + strand, within PP_4945 at 5628.391 kb on + strand, within PP_4945 at 5628.392 kb on - strand at 5628.392 kb on - strand, within PP_4945 at 5628.419 kb on + strand, within PP_4945 at 5628.421 kb on + strand, within PP_4945 at 5628.523 kb on + strand at 5628.523 kb on + strand at 5628.523 kb on + strand at 5628.524 kb on - strand at 5628.558 kb on - strand at 5628.558 kb on - strand at 5628.638 kb on + strand at 5628.638 kb on + strand at 5628.638 kb on + strand at 5628.639 kb on - strand at 5628.639 kb on - strand at 5628.639 kb on - strand at 5628.765 kb on - strand at 5628.914 kb on + strand, within PP_4946 at 5628.914 kb on + strand, within PP_4946 at 5629.049 kb on + strand, within PP_4946 at 5629.049 kb on + strand, within PP_4946 at 5629.049 kb on + strand, within PP_4946 at 5629.101 kb on + strand, within PP_4946 at 5629.102 kb on - strand, within PP_4946 at 5629.239 kb on - strand, within PP_4946 at 5629.239 kb on - strand, within PP_4946
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 5,626,730 - PP_4944 0.56 +0.6 5,626,730 - PP_4944 0.56 +2.2 5,626,730 - PP_4944 0.56 -0.6 5,626,928 - PP_4944 0.67 +0.6 5,627,075 - PP_4944 0.76 +0.5 5,627,252 - PP_4944 0.86 +0.1 5,627,508 - +0.8 5,627,574 - +0.2 5,627,608 + +2.2 5,627,609 - -0.9 5,627,658 + -0.7 5,627,695 + +1.2 5,627,726 + +2.2 5,627,860 + PP_4945 0.20 -1.1 5,627,860 + PP_4945 0.20 +0.6 5,627,860 + PP_4945 0.20 +0.3 5,627,860 + PP_4945 0.20 -1.1 5,627,861 - PP_4945 0.20 -0.7 5,627,861 - PP_4945 0.20 -0.4 5,627,861 - PP_4945 0.20 -1.1 5,627,861 - PP_4945 0.20 -0.1 5,627,861 - PP_4945 0.20 -0.4 5,627,912 - PP_4945 0.26 +3.0 5,627,912 - PP_4945 0.26 -0.7 5,627,915 - PP_4945 0.26 -0.2 5,628,185 + PP_4945 0.59 -0.1 5,628,247 + PP_4945 0.66 +1.9 5,628,248 - PP_4945 0.66 +0.7 5,628,292 + PP_4945 0.71 -0.3 5,628,293 - PP_4945 0.72 +0.2 5,628,391 + PP_4945 0.83 -0.2 5,628,391 + PP_4945 0.83 -2.1 5,628,391 + PP_4945 0.83 +2.2 5,628,391 + PP_4945 0.83 -0.2 5,628,392 - +1.2 5,628,392 - PP_4945 0.83 -0.0 5,628,419 + PP_4945 0.87 +1.7 5,628,421 + PP_4945 0.87 +0.4 5,628,523 + -0.2 5,628,523 + -2.2 5,628,523 + -0.5 5,628,524 - +1.7 5,628,558 - -0.4 5,628,558 - +0.8 5,628,638 + -0.4 5,628,638 + +0.3 5,628,638 + +0.5 5,628,639 - +0.6 5,628,639 - -1.0 5,628,639 - -0.4 5,628,765 - +0.6 5,628,914 + PP_4946 0.17 +1.1 5,628,914 + PP_4946 0.17 +0.3 5,629,049 + PP_4946 0.25 +0.0 5,629,049 + PP_4946 0.25 +2.2 5,629,049 + PP_4946 0.25 +2.5 5,629,101 + PP_4946 0.28 +0.5 5,629,102 - PP_4946 0.29 +0.3 5,629,239 - PP_4946 0.37 -0.3 5,629,239 - PP_4946 0.37 +0.1
Or see this region's nucleotide sequence