Strain Fitness in Pseudomonas putida KT2440 around PP_4406

Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
4,997,427	+		PP_4405	0.33	-0.9
4,997,758	+		PP_4405	0.66	+0.1
4,997,796	+		PP_4405	0.69	+1.1
4,997,796	+		PP_4405	0.69	+0.3
4,997,796	+		PP_4405	0.69	+0.8
4,997,796	+		PP_4405	0.69	-0.1
4,997,796	+		PP_4405	0.69	+0.1
4,997,796	+		PP_4405	0.69	-0.1
4,997,797	-		PP_4405	0.70	-0.4
4,997,823	+		PP_4405	0.72	+1.0
4,997,823	+		PP_4405	0.72	-0.2
4,997,824	-		PP_4405	0.72	+1.3
4,997,859	+		PP_4405	0.76	-0.5
4,997,859	+		PP_4405	0.76	+0.5
4,997,859	+		PP_4405	0.76	+1.5
4,997,859	+		PP_4405	0.76	+0.5
4,997,859	+		PP_4405	0.76	-0.9
4,997,889	+		PP_4405	0.79	+0.2
4,997,890	-		PP_4405	0.79	-0.4
4,997,920	+		PP_4405	0.82	+0.2
4,997,920	+		PP_4405	0.82	+0.2
4,997,921	-		PP_4405	0.82	+1.3
4,997,952	+		PP_4405	0.85	-1.0
4,997,967	+		PP_4405	0.87	+0.9
4,997,968	-		PP_4405	0.87	-0.6
4,997,992	-		PP_4405	0.89	+0.9
4,997,992	-		PP_4405	0.89	+0.8
4,998,077	+				+0.8
4,998,189	+				+0.1
4,998,204	+				+0.9
4,998,204	+				+0.4
4,998,204	+				-0.9
4,998,204	+				-0.4
4,998,205	-				+0.6
4,998,205	-				+1.1
4,998,205	-				-1.7
4,998,205	-				+0.3
4,998,205	-				-1.4
4,998,205	-				+0.5
4,998,205	-				+0.3
4,998,205	-				+0.2
4,998,320	+				+0.3
4,998,320	+				-0.0
4,998,321	-				-0.3
4,998,370	+		PP_4406	0.10	+0.5
4,998,536	+		PP_4406	0.42	+0.3
4,998,682	+		PP_4406	0.69	+0.4
4,998,868	+				+0.2
4,998,868	+				-0.5
4,999,033	+				-1.3
4,999,033	+				-0.1
4,999,033	+				-1.8
4,999,034	-				-0.1
4,999,034	-				-0.6
4,999,034	-				+0.7
4,999,056	+				-2.0
4,999,056	+				+0.6
4,999,056	+				+0.3
4,999,056	+				-0.2
4,999,057	-				+1.1
4,999,060	+				-1.1
4,999,060	+				+0.8
4,999,061	-				-0.1
4,999,061	-				-1.0
4,999,061	-				-0.2
4,999,061	-				+0.3
4,999,189	+				+0.7
4,999,189	+				+0.1
4,999,190	-				-0.5
4,999,190	-				-2.0
4,999,190	-				-0.2
4,999,190	-				-0.8
4,999,278	+				+0.3
4,999,279	-				+0.0
4,999,286	+				+0.2
4,999,376	+		PP_4407	0.16	+1.1
4,999,389	-		PP_4407	0.17	-0.0
4,999,389	-		PP_4407	0.17	+0.1
4,999,389	-		PP_4407	0.17	+0.1
4,999,562	+		PP_4407	0.30	+0.6
4,999,587	+		PP_4407	0.32	-0.4
4,999,592	+		PP_4407	0.32	-0.5
4,999,593	-		PP_4407	0.32	-2.9
4,999,593	-		PP_4407	0.32	-0.0
4,999,627	+		PP_4407	0.35	+0.2
4,999,627	+		PP_4407	0.35	-1.2
4,999,627	+		PP_4407	0.35	-0.1
4,999,628	-		PP_4407	0.35	-0.2
4,999,628	-		PP_4407	0.35	+0.3
4,999,628	-		PP_4407	0.35	+0.8
4,999,675	+		PP_4407	0.38	+0.1
4,999,676	-		PP_4407	0.38	+0.1
4,999,676	-		PP_4407	0.38	-0.1
4,999,676	-		PP_4407	0.38	+0.2
4,999,676	-		PP_4407	0.38	-0.4
4,999,676	-		PP_4407	0.38	+0.2