Strain Fitness in Pseudomonas putida KT2440 around PP_4009

Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
4,518,771	+		PP_4008	0.67	+0.2
4,518,771	+		PP_4008	0.67	-0.0
4,518,771	+		PP_4008	0.67	+0.3
4,518,771	+		PP_4008	0.67	-0.3
4,518,772	-		PP_4008	0.67	-1.8
4,518,941	+		PP_4008	0.75	+0.2
4,518,942	-		PP_4008	0.75	+1.8
4,518,993	+		PP_4008	0.77	-0.2
4,519,110	+		PP_4008	0.82	-1.4
4,519,110	+		PP_4008	0.82	-0.8
4,519,110	+		PP_4008	0.82	-0.5
4,519,111	-		PP_4008	0.82	+1.8
4,519,111	-		PP_4008	0.82	+2.8
4,519,515	-				+0.5
4,519,519	+				+0.8
4,519,519	+				+1.8
4,519,519	+				+0.9
4,519,519	+				+0.8
4,519,519	+				-0.6
4,519,519	+				+0.1
4,519,519	+				+0.5
4,519,519	+				+0.3
4,519,520	-				+1.0
4,519,520	-				-1.8
4,519,520	-				+1.0
4,519,520	-				+0.2
4,519,532	+				+0.2
4,519,533	-				+0.3
4,519,533	-				+1.6
4,519,578	-				-0.5
4,519,645	+		PP_4009	0.26	+0.2
4,519,765	-		PP_4009	0.59	+0.2
4,519,765	-		PP_4009	0.59	+2.0
4,519,791	+		PP_4009	0.66	+0.8
4,519,791	+		PP_4009	0.66	+0.8
4,519,792	-		PP_4009	0.67	+0.1
4,519,792	-		PP_4009	0.67	+0.9
4,519,900	+				-0.8
4,519,900	+				+1.0
4,519,901	-				+0.2
4,520,016	+				-1.2
4,520,017	-				-1.0
4,520,025	+				-0.7
4,520,026	-				+1.0
4,520,026	-				-0.4
4,520,105	+				-1.2
4,520,105	+				-1.3
4,520,105	+				-1.2
4,520,105	+				-1.6
4,520,106	-				+0.7
4,520,138	+				-0.3
4,520,139	-				-0.3
4,520,139	-				+0.1
4,520,139	-				+1.8
4,520,185	+		PP_4010	0.16	-0.0
4,520,208	+		PP_4010	0.24	+0.3
4,520,233	+		PP_4010	0.34	+0.8
4,520,258	-		PP_4010	0.43	+0.1
4,520,258	-		PP_4010	0.43	-0.0
4,520,289	-		PP_4010	0.55	+0.2
4,520,289	-		PP_4010	0.55	+0.9
4,520,454	-				+0.1
4,520,482	+				-0.7
4,520,482	+				-1.9
4,520,482	+				+0.2
4,520,482	+				-1.1
4,520,483	-				+1.2
4,520,483	-				-0.5
4,520,483	-				-0.7
4,520,483	-				+0.7
4,520,483	-				+0.4
4,520,523	+				+1.0
4,520,560	+				-0.1
4,520,561	-				+0.6
4,520,698	+		PP_4011	0.17	+0.3
4,520,699	-		PP_4011	0.17	+0.0
4,520,699	-		PP_4011	0.17	+1.4
4,520,699	-		PP_4011	0.17	-0.2
4,520,699	-		PP_4011	0.17	+0.6
4,520,809	+		PP_4011	0.26	+0.6
4,520,809	+		PP_4011	0.26	+0.3
4,520,810	-		PP_4011	0.26	+0.3
4,520,810	-		PP_4011	0.26	-1.8
4,520,846	-		PP_4011	0.29	+0.4