Strain Fitness in Pseudomonas putida KT2440 around PP_3534

Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
4,004,230	+		PP_3533	0.19	+0.8
4,004,231	-		PP_3533	0.19	+0.7
4,004,242	+		PP_3533	0.20	+0.4
4,004,242	+		PP_3533	0.20	+0.3
4,004,243	-		PP_3533	0.20	-0.6
4,004,283	+		PP_3533	0.24	+0.3
4,004,284	-		PP_3533	0.24	-0.9
4,004,284	-		PP_3533	0.24	+0.1
4,004,284	-		PP_3533	0.24	-1.0
4,004,303	+				+0.6
4,004,303	+		PP_3533	0.25	-0.1
4,004,563	+		PP_3533	0.50	-0.3
4,004,563	+		PP_3533	0.50	+0.4
4,004,692	-		PP_3533	0.62	-0.4
4,004,779	+		PP_3533	0.71	-0.8
4,004,780	-		PP_3533	0.71	-1.1
4,004,903	+		PP_3533	0.82	+0.6
4,004,904	-		PP_3533	0.83	+0.3
4,004,945	+		PP_3533	0.86	+0.3
4,004,954	+		PP_3533	0.87	+0.2
4,004,955	-		PP_3533	0.87	+0.9
4,004,955	-		PP_3533	0.87	+0.5
4,004,958	-		PP_3533	0.88	-0.4
4,005,069	+				+0.8
4,005,088	+				-1.2
4,005,089	-				+1.0
4,005,135	+				+0.2
4,005,135	+				+2.3
4,005,135	+				-1.6
4,005,135	+				+0.1
4,005,135	+				-0.7
4,005,136	-				-1.4
4,005,136	-				-0.1
4,005,136	-				+1.3
4,005,136	-				+2.0
4,005,253	+		PP_3534	0.11	-1.9
4,005,253	+		PP_3534	0.11	-0.2
4,005,254	-		PP_3534	0.11	-0.3
4,005,254	-		PP_3534	0.11	+1.6
4,005,377	+		PP_3534	0.25	-0.3
4,005,377	+		PP_3534	0.25	-0.1
4,005,386	+		PP_3534	0.26	+0.2
4,005,387	-		PP_3534	0.26	-0.7
4,005,607	+		PP_3534	0.51	-0.6
4,005,608	-		PP_3534	0.51	+1.1
4,005,608	-		PP_3534	0.51	-2.2
4,005,608	-		PP_3534	0.51	-0.7
4,005,698	+		PP_3534	0.61	-0.7
4,005,699	-		PP_3534	0.61	+0.1
4,005,843	+		PP_3534	0.78	-0.7
4,005,843	+		PP_3534	0.78	-1.3
4,005,844	-		PP_3534	0.78	-2.1
4,006,035	-				+0.4
4,006,042	+				+0.7
4,006,042	+				-1.2
4,006,042	+				+0.2
4,006,042	+				+0.6
4,006,042	+				-0.2
4,006,043	-				+0.7
4,006,046	+				-0.0
4,006,046	+				-0.0
4,006,047	-				-0.6
4,006,078	-				-1.2
4,006,749	+		PP_3535	0.37	-0.6
4,006,749	+		PP_3535	0.37	-0.4
4,006,750	-				+1.6
4,006,750	-		PP_3535	0.37	-0.1
4,006,788	+		PP_3535	0.39	-0.8
4,006,788	+		PP_3535	0.39	-0.4
4,006,788	+		PP_3535	0.39	-1.6
4,006,788	+		PP_3535	0.39	+1.4
4,006,789	-		PP_3535	0.39	-0.2
4,006,789	-		PP_3535	0.39	+0.6
4,006,806	+		PP_3535	0.40	+0.2
4,006,807	-		PP_3535	0.40	+1.6
4,006,973	+		PP_3535	0.50	-0.0
4,007,031	+		PP_3535	0.53	-0.5
4,007,031	+		PP_3535	0.53	+0.4
4,007,032	-		PP_3535	0.53	+0.0