Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3478 and PP_5605 overlap by 4 nucleotides PP_5605 and PP_3480 overlap by 1 nucleotides PP_3480 and PP_3481 overlap by 14 nucleotides PP_3481 and PP_3482 are separated by 1 nucleotides
PP_3478: PP_3478 - putative Secretion protein, at 3,942,027 to 3,943,892
_3478
PP_5605: PP_5605 - conserved exported protein of unknown function, at 3,943,889 to 3,944,395
_5605
PP_3480: PP_3480 - conserved protein of unknown function, at 3,944,395 to 3,944,958
_3480
PP_3481: PP_3481 - conserved exported protein of unknown function, at 3,944,945 to 3,945,487
_3481
PP_3482: PP_3482 - conserved protein of unknown function, at 3,945,489 to 3,946,298
_3482
Position (kb)
3944
3945 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3943.459 kb on + strand, within PP_3478 at 3943.460 kb on - strand, within PP_3478 at 3943.460 kb on - strand, within PP_3478 at 3943.512 kb on + strand, within PP_3478 at 3943.604 kb on - strand, within PP_3478 at 3943.716 kb on - strand at 3943.768 kb on + strand at 3943.769 kb on - strand at 3943.769 kb on - strand at 3943.939 kb on - strand at 3943.939 kb on - strand at 3943.980 kb on + strand, within PP_5605 at 3943.980 kb on + strand, within PP_5605 at 3943.980 kb on + strand, within PP_5605 at 3943.981 kb on - strand, within PP_5605 at 3943.981 kb on - strand, within PP_5605 at 3944.243 kb on + strand, within PP_5605 at 3944.243 kb on + strand, within PP_5605 at 3944.243 kb on + strand, within PP_5605 at 3944.243 kb on + strand, within PP_5605 at 3944.244 kb on - strand, within PP_5605 at 3944.269 kb on + strand, within PP_5605 at 3944.269 kb on + strand, within PP_5605 at 3944.270 kb on - strand, within PP_5605 at 3944.412 kb on - strand at 3944.412 kb on - strand at 3944.412 kb on - strand at 3944.520 kb on + strand, within PP_3480 at 3944.520 kb on + strand, within PP_3480 at 3944.521 kb on - strand, within PP_3480 at 3944.610 kb on - strand, within PP_3480 at 3944.773 kb on + strand, within PP_3480 at 3945.041 kb on + strand, within PP_3481 at 3945.041 kb on + strand, within PP_3481 at 3945.081 kb on + strand, within PP_3481 at 3945.599 kb on - strand, within PP_3482 at 3945.635 kb on + strand, within PP_3482 at 3945.727 kb on - strand, within PP_3482 at 3945.727 kb on - strand, within PP_3482 at 3945.727 kb on - strand, within PP_3482 at 3945.727 kb on - strand, within PP_3482 at 3945.796 kb on + strand at 3945.796 kb on + strand, within PP_3482 at 3945.796 kb on + strand, within PP_3482 at 3945.796 kb on + strand, within PP_3482 at 3945.796 kb on + strand, within PP_3482 at 3945.796 kb on + strand, within PP_3482 at 3945.797 kb on - strand, within PP_3482 at 3945.797 kb on - strand, within PP_3482 at 3945.797 kb on - strand, within PP_3482 at 3945.797 kb on - strand, within PP_3482 at 3945.797 kb on - strand, within PP_3482 at 3945.811 kb on + strand, within PP_3482 at 3945.811 kb on + strand, within PP_3482 at 3945.812 kb on - strand, within PP_3482 at 3945.948 kb on + strand, within PP_3482
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 3,943,459 + PP_3478 0.77 +1.1 3,943,460 - PP_3478 0.77 -0.4 3,943,460 - PP_3478 0.77 -0.3 3,943,512 + PP_3478 0.80 +1.1 3,943,604 - PP_3478 0.85 +1.1 3,943,716 - -0.8 3,943,768 + -0.8 3,943,769 - -1.6 3,943,769 - +0.3 3,943,939 - +1.0 3,943,939 - +1.0 3,943,980 + PP_5605 0.18 +1.0 3,943,980 + PP_5605 0.18 +1.5 3,943,980 + PP_5605 0.18 -1.2 3,943,981 - PP_5605 0.18 +1.9 3,943,981 - PP_5605 0.18 +2.0 3,944,243 + PP_5605 0.70 +0.6 3,944,243 + PP_5605 0.70 -1.0 3,944,243 + PP_5605 0.70 +0.0 3,944,243 + PP_5605 0.70 -0.3 3,944,244 - PP_5605 0.70 -2.0 3,944,269 + PP_5605 0.75 -1.3 3,944,269 + PP_5605 0.75 -0.6 3,944,270 - PP_5605 0.75 -0.5 3,944,412 - -0.4 3,944,412 - -0.4 3,944,412 - +0.1 3,944,520 + PP_3480 0.22 +0.1 3,944,520 + PP_3480 0.22 +1.0 3,944,521 - PP_3480 0.22 -0.9 3,944,610 - PP_3480 0.38 +0.2 3,944,773 + PP_3480 0.67 +0.5 3,945,041 + PP_3481 0.18 -0.3 3,945,041 + PP_3481 0.18 +0.5 3,945,081 + PP_3481 0.25 -1.3 3,945,599 - PP_3482 0.14 -0.7 3,945,635 + PP_3482 0.18 -0.7 3,945,727 - PP_3482 0.29 -0.3 3,945,727 - PP_3482 0.29 -0.7 3,945,727 - PP_3482 0.29 -1.0 3,945,727 - PP_3482 0.29 -1.0 3,945,796 + -0.6 3,945,796 + PP_3482 0.38 +0.4 3,945,796 + PP_3482 0.38 -0.9 3,945,796 + PP_3482 0.38 -2.5 3,945,796 + PP_3482 0.38 +0.8 3,945,796 + PP_3482 0.38 -0.4 3,945,797 - PP_3482 0.38 +0.2 3,945,797 - PP_3482 0.38 -0.4 3,945,797 - PP_3482 0.38 +0.3 3,945,797 - PP_3482 0.38 +0.1 3,945,797 - PP_3482 0.38 +0.3 3,945,811 + PP_3482 0.40 -1.3 3,945,811 + PP_3482 0.40 +1.7 3,945,812 - PP_3482 0.40 -0.5 3,945,948 + PP_3482 0.57 +0.9
Or see this region's nucleotide sequence