Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2803 and PP_2804 are separated by 191 nucleotides PP_2804 and PP_2805 overlap by 4 nucleotides
PP_2803: PP_2803 - 1,3-propanediol dehydrogenase, at 3,194,882 to 3,196,066
_2803
PP_2804: PP_2804 - Hydrolase, alpha/beta fold family, at 3,196,258 to 3,197,133
_2804
PP_2805: PP_2805 - FAD-containing monooxygenase EthA, at 3,197,130 to 3,198,656
_2805
Position (kb)
3196
3197
3198 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3195.435 kb on + strand, within PP_2803 at 3195.436 kb on - strand, within PP_2803 at 3195.436 kb on - strand, within PP_2803 at 3195.547 kb on - strand, within PP_2803 at 3195.685 kb on - strand, within PP_2803 at 3195.685 kb on - strand at 3195.685 kb on - strand, within PP_2803 at 3196.023 kb on - strand at 3196.039 kb on + strand at 3196.039 kb on + strand at 3196.039 kb on + strand at 3196.043 kb on + strand at 3196.043 kb on + strand at 3196.044 kb on - strand at 3196.044 kb on - strand at 3196.044 kb on - strand at 3196.110 kb on - strand at 3196.125 kb on + strand at 3196.125 kb on + strand at 3196.126 kb on - strand at 3196.126 kb on - strand at 3196.674 kb on + strand, within PP_2804 at 3196.674 kb on + strand, within PP_2804 at 3196.675 kb on - strand, within PP_2804 at 3196.675 kb on - strand, within PP_2804 at 3196.869 kb on - strand, within PP_2804 at 3197.016 kb on - strand, within PP_2804 at 3197.255 kb on - strand at 3197.319 kb on + strand, within PP_2805 at 3197.319 kb on + strand, within PP_2805 at 3197.320 kb on - strand, within PP_2805 at 3197.320 kb on - strand, within PP_2805 at 3197.323 kb on + strand, within PP_2805 at 3197.393 kb on - strand, within PP_2805 at 3197.625 kb on - strand, within PP_2805 at 3197.658 kb on + strand, within PP_2805 at 3197.658 kb on + strand, within PP_2805 at 3197.659 kb on - strand, within PP_2805 at 3198.001 kb on + strand, within PP_2805 at 3198.001 kb on + strand, within PP_2805 at 3198.001 kb on + strand, within PP_2805 at 3198.002 kb on - strand, within PP_2805 at 3198.002 kb on - strand, within PP_2805 at 3198.002 kb on - strand, within PP_2805 at 3198.112 kb on + strand, within PP_2805 at 3198.113 kb on - strand, within PP_2805
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 3,195,435 + PP_2803 0.47 -0.2 3,195,436 - PP_2803 0.47 +0.4 3,195,436 - PP_2803 0.47 -0.0 3,195,547 - PP_2803 0.56 +0.1 3,195,685 - PP_2803 0.68 -0.2 3,195,685 - +0.2 3,195,685 - PP_2803 0.68 +0.4 3,196,023 - +0.2 3,196,039 + +0.5 3,196,039 + -1.4 3,196,039 + -0.0 3,196,043 + +1.1 3,196,043 + +0.0 3,196,044 - -2.4 3,196,044 - +0.3 3,196,044 - -0.5 3,196,110 - -0.4 3,196,125 + -1.0 3,196,125 + +2.8 3,196,126 - +0.2 3,196,126 - +1.2 3,196,674 + PP_2804 0.47 +0.7 3,196,674 + PP_2804 0.47 +0.5 3,196,675 - PP_2804 0.48 -0.2 3,196,675 - PP_2804 0.48 +0.6 3,196,869 - PP_2804 0.70 +1.4 3,197,016 - PP_2804 0.87 -0.4 3,197,255 - +0.2 3,197,319 + PP_2805 0.12 -0.9 3,197,319 + PP_2805 0.12 +0.1 3,197,320 - PP_2805 0.12 -0.3 3,197,320 - PP_2805 0.12 +1.2 3,197,323 + PP_2805 0.13 -2.9 3,197,393 - PP_2805 0.17 +0.0 3,197,625 - PP_2805 0.32 -0.1 3,197,658 + PP_2805 0.35 -1.8 3,197,658 + PP_2805 0.35 -1.1 3,197,659 - PP_2805 0.35 -0.3 3,198,001 + PP_2805 0.57 +0.8 3,198,001 + PP_2805 0.57 -2.2 3,198,001 + PP_2805 0.57 -0.2 3,198,002 - PP_2805 0.57 -0.2 3,198,002 - PP_2805 0.57 +0.5 3,198,002 - PP_2805 0.57 -0.2 3,198,112 + PP_2805 0.64 -0.3 3,198,113 - PP_2805 0.64 -0.6
Or see this region's nucleotide sequence