Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2187 and PP_2188 are separated by 154 nucleotides PP_2188 and PP_2189 are separated by 22 nucleotides PP_2189 and PP_2190 are separated by 22 nucleotides PP_2190 and PP_2191 are separated by 20 nucleotides PP_2191 and PP_2192 are separated by 131 nucleotides
PP_2187: PP_2187 - Universal stress protein family, at 2,493,774 to 2,494,637
_2187
PP_2188: PP_2188 - putative tRNA-(Ms(2)io(6)a)-hydroxylase, at 2,494,792 to 2,495,409
_2188
PP_2189: PP_2189 - conserved protein of unknown function, at 2,495,432 to 2,495,857
_2189
PP_2190: PP_2190 - conserved protein of unknown function, at 2,495,880 to 2,496,143
_2190
PP_2191: PP_2191 - putative Lipoprotein, at 2,496,164 to 2,496,643
_2191
PP_2192: PP_2192 - putative RNA polymerase sigma-70 factor, ECF subfamily/transmembrane sensor protein, at 2,496,775 to 2,497,899
_2192
Position (kb)
2495
2496 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2494.575 kb on - strand at 2494.678 kb on + strand at 2494.679 kb on - strand at 2494.680 kb on + strand at 2494.711 kb on + strand at 2494.712 kb on - strand at 2494.722 kb on + strand at 2494.722 kb on + strand at 2494.723 kb on - strand at 2494.723 kb on - strand at 2494.723 kb on - strand at 2494.723 kb on - strand at 2494.753 kb on + strand at 2494.754 kb on - strand at 2494.791 kb on + strand at 2494.792 kb on - strand at 2494.792 kb on - strand at 2494.945 kb on + strand, within PP_2188 at 2494.946 kb on - strand, within PP_2188 at 2494.946 kb on - strand, within PP_2188 at 2494.946 kb on - strand, within PP_2188 at 2495.021 kb on + strand, within PP_2188 at 2495.021 kb on + strand, within PP_2188 at 2495.022 kb on - strand, within PP_2188 at 2495.130 kb on + strand, within PP_2188 at 2495.222 kb on - strand, within PP_2188 at 2495.222 kb on - strand, within PP_2188 at 2495.284 kb on + strand, within PP_2188 at 2495.284 kb on + strand, within PP_2188 at 2495.284 kb on + strand, within PP_2188 at 2495.324 kb on + strand, within PP_2188 at 2495.434 kb on - strand at 2495.450 kb on + strand at 2495.451 kb on - strand at 2495.517 kb on + strand, within PP_2189 at 2495.518 kb on - strand, within PP_2189 at 2495.675 kb on + strand, within PP_2189 at 2495.676 kb on - strand, within PP_2189 at 2495.676 kb on - strand, within PP_2189 at 2495.843 kb on + strand at 2495.843 kb on + strand at 2495.844 kb on - strand at 2495.844 kb on - strand at 2495.844 kb on - strand at 2495.848 kb on + strand at 2495.873 kb on + strand at 2495.874 kb on - strand at 2495.874 kb on - strand at 2496.081 kb on + strand, within PP_2190 at 2496.168 kb on + strand at 2496.168 kb on + strand at 2496.168 kb on + strand at 2496.169 kb on - strand at 2496.169 kb on - strand at 2496.255 kb on + strand, within PP_2191 at 2496.255 kb on + strand, within PP_2191 at 2496.255 kb on + strand, within PP_2191 at 2496.256 kb on - strand, within PP_2191 at 2496.256 kb on - strand, within PP_2191 at 2496.367 kb on + strand, within PP_2191 at 2496.367 kb on + strand, within PP_2191 at 2496.367 kb on + strand, within PP_2191 at 2496.368 kb on - strand, within PP_2191 at 2496.368 kb on - strand, within PP_2191 at 2496.441 kb on + strand, within PP_2191 at 2496.627 kb on + strand at 2496.629 kb on + strand at 2496.630 kb on - strand at 2496.813 kb on - strand at 2496.823 kb on + strand at 2496.824 kb on - strand at 2496.824 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 2,494,575 - -1.8 2,494,678 + -0.2 2,494,679 - +0.1 2,494,680 + -0.1 2,494,711 + +0.5 2,494,712 - +0.5 2,494,722 + -0.8 2,494,722 + -0.2 2,494,723 - -0.4 2,494,723 - -0.4 2,494,723 - -0.0 2,494,723 - +0.2 2,494,753 + +0.2 2,494,754 - -0.1 2,494,791 + +0.4 2,494,792 - -0.2 2,494,792 - +0.1 2,494,945 + PP_2188 0.25 +1.1 2,494,946 - PP_2188 0.25 +0.9 2,494,946 - PP_2188 0.25 +0.3 2,494,946 - PP_2188 0.25 +0.2 2,495,021 + PP_2188 0.37 +0.2 2,495,021 + PP_2188 0.37 -0.2 2,495,022 - PP_2188 0.37 -0.1 2,495,130 + PP_2188 0.55 -0.3 2,495,222 - PP_2188 0.70 -0.0 2,495,222 - PP_2188 0.70 -0.7 2,495,284 + PP_2188 0.80 -1.2 2,495,284 + PP_2188 0.80 -0.2 2,495,284 + PP_2188 0.80 -1.3 2,495,324 + PP_2188 0.86 +0.5 2,495,434 - +0.5 2,495,450 + -0.6 2,495,451 - +1.2 2,495,517 + PP_2189 0.20 +0.2 2,495,518 - PP_2189 0.20 -0.2 2,495,675 + PP_2189 0.57 +0.3 2,495,676 - PP_2189 0.57 +1.8 2,495,676 - PP_2189 0.57 +0.7 2,495,843 + -1.5 2,495,843 + -0.6 2,495,844 - +0.4 2,495,844 - -0.4 2,495,844 - +0.2 2,495,848 + +0.5 2,495,873 + -0.5 2,495,874 - -0.9 2,495,874 - +0.3 2,496,081 + PP_2190 0.76 +1.4 2,496,168 + -0.3 2,496,168 + +0.4 2,496,168 + -0.4 2,496,169 - +1.4 2,496,169 - -0.2 2,496,255 + PP_2191 0.19 -0.0 2,496,255 + PP_2191 0.19 +1.4 2,496,255 + PP_2191 0.19 +0.5 2,496,256 - PP_2191 0.19 +0.6 2,496,256 - PP_2191 0.19 +0.5 2,496,367 + PP_2191 0.42 +0.2 2,496,367 + PP_2191 0.42 -1.5 2,496,367 + PP_2191 0.42 +0.6 2,496,368 - PP_2191 0.42 -0.3 2,496,368 - PP_2191 0.42 -0.3 2,496,441 + PP_2191 0.58 -0.2 2,496,627 + +0.1 2,496,629 + -0.3 2,496,630 - -0.2 2,496,813 - +0.2 2,496,823 + +0.2 2,496,824 - +0.4 2,496,824 - -0.8
Or see this region's nucleotide sequence