Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1179 and PP_1180 are separated by 93 nucleotides PP_1180 and PP_1181 are separated by 391 nucleotides PP_1181 and PP_1182 overlap by 4 nucleotides
PP_1179: PP_1179 - Ribonucleoside-diphosphate reductase subunit alpha, at 1,352,497 to 1,355,376
_1179
PP_1180: PP_1180 - conserved protein of unknown function, at 1,355,470 to 1,355,733
_1180
PP_1181: PP_1181 - DNA-binding response regulator, at 1,356,125 to 1,356,823
_1181
PP_1182: PP_1182 - Sensor histidine kinase, at 1,356,820 to 1,358,427
_1182
Position (kb)
1356
1357 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1355.400 kb on - strand at 1355.402 kb on - strand at 1355.402 kb on - strand at 1355.418 kb on - strand at 1355.765 kb on - strand at 1355.765 kb on - strand at 1355.769 kb on - strand at 1355.798 kb on - strand at 1355.838 kb on + strand at 1355.849 kb on + strand at 1355.849 kb on + strand at 1355.850 kb on - strand at 1355.850 kb on - strand at 1356.069 kb on + strand at 1356.069 kb on + strand at 1356.069 kb on + strand at 1356.069 kb on + strand at 1356.069 kb on + strand at 1356.069 kb on + strand at 1356.069 kb on + strand at 1356.069 kb on + strand at 1356.070 kb on - strand at 1356.070 kb on - strand at 1356.078 kb on - strand at 1356.078 kb on - strand at 1356.078 kb on - strand at 1356.194 kb on + strand at 1356.348 kb on - strand, within PP_1181 at 1356.428 kb on + strand, within PP_1181 at 1356.428 kb on + strand, within PP_1181 at 1356.429 kb on - strand, within PP_1181 at 1356.429 kb on - strand, within PP_1181 at 1356.429 kb on - strand, within PP_1181 at 1356.429 kb on - strand, within PP_1181 at 1356.429 kb on - strand, within PP_1181 at 1356.469 kb on + strand, within PP_1181 at 1356.469 kb on + strand, within PP_1181 at 1356.470 kb on - strand, within PP_1181 at 1356.470 kb on - strand, within PP_1181 at 1356.716 kb on - strand, within PP_1181 at 1356.797 kb on + strand at 1356.848 kb on - strand at 1357.108 kb on + strand, within PP_1182 at 1357.108 kb on + strand, within PP_1182 at 1357.108 kb on + strand, within PP_1182 at 1357.109 kb on - strand, within PP_1182 at 1357.109 kb on - strand, within PP_1182 at 1357.160 kb on + strand, within PP_1182 at 1357.160 kb on + strand, within PP_1182 at 1357.240 kb on + strand, within PP_1182 at 1357.241 kb on - strand, within PP_1182 at 1357.241 kb on - strand, within PP_1182 at 1357.241 kb on - strand, within PP_1182 at 1357.241 kb on - strand, within PP_1182 at 1357.241 kb on - strand, within PP_1182 at 1357.241 kb on - strand, within PP_1182 at 1357.244 kb on + strand, within PP_1182 at 1357.244 kb on + strand, within PP_1182 at 1357.245 kb on - strand, within PP_1182 at 1357.245 kb on - strand, within PP_1182 at 1357.245 kb on - strand, within PP_1182 at 1357.245 kb on - strand, within PP_1182 at 1357.347 kb on + strand, within PP_1182 at 1357.347 kb on + strand, within PP_1182 at 1357.347 kb on + strand, within PP_1182 at 1357.348 kb on - strand, within PP_1182 at 1357.348 kb on - strand, within PP_1182 at 1357.493 kb on - strand, within PP_1182 at 1357.570 kb on + strand, within PP_1182 at 1357.570 kb on + strand, within PP_1182
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 1,355,400 - -0.6 1,355,402 - +0.5 1,355,402 - +0.3 1,355,418 - +1.0 1,355,765 - -1.0 1,355,765 - -0.8 1,355,769 - -1.5 1,355,798 - -0.9 1,355,838 + +0.3 1,355,849 + -1.3 1,355,849 + -0.7 1,355,850 - +0.1 1,355,850 - -0.8 1,356,069 + -0.1 1,356,069 + +1.0 1,356,069 + +0.6 1,356,069 + -0.3 1,356,069 + -1.4 1,356,069 + +0.3 1,356,069 + +0.0 1,356,069 + -1.0 1,356,070 - -0.4 1,356,070 - -0.8 1,356,078 - +0.3 1,356,078 - +0.2 1,356,078 - -0.4 1,356,194 + +0.3 1,356,348 - PP_1181 0.32 -0.0 1,356,428 + PP_1181 0.43 +1.0 1,356,428 + PP_1181 0.43 +3.0 1,356,429 - PP_1181 0.43 +0.8 1,356,429 - PP_1181 0.43 +1.0 1,356,429 - PP_1181 0.43 +0.0 1,356,429 - PP_1181 0.43 +0.1 1,356,429 - PP_1181 0.43 +0.1 1,356,469 + PP_1181 0.49 -2.0 1,356,469 + PP_1181 0.49 -0.1 1,356,470 - PP_1181 0.49 +1.3 1,356,470 - PP_1181 0.49 +0.2 1,356,716 - PP_1181 0.85 +0.4 1,356,797 + +0.3 1,356,848 - -0.5 1,357,108 + PP_1182 0.18 +0.1 1,357,108 + PP_1182 0.18 -0.1 1,357,108 + PP_1182 0.18 -0.1 1,357,109 - PP_1182 0.18 -0.5 1,357,109 - PP_1182 0.18 +0.3 1,357,160 + PP_1182 0.21 +0.7 1,357,160 + PP_1182 0.21 +0.6 1,357,240 + PP_1182 0.26 -0.3 1,357,241 - PP_1182 0.26 +0.8 1,357,241 - PP_1182 0.26 +0.6 1,357,241 - PP_1182 0.26 +0.1 1,357,241 - PP_1182 0.26 +0.2 1,357,241 - PP_1182 0.26 +0.1 1,357,241 - PP_1182 0.26 -0.4 1,357,244 + PP_1182 0.26 +1.0 1,357,244 + PP_1182 0.26 -0.1 1,357,245 - PP_1182 0.26 -0.1 1,357,245 - PP_1182 0.26 +0.3 1,357,245 - PP_1182 0.26 +0.6 1,357,245 - PP_1182 0.26 +0.1 1,357,347 + PP_1182 0.33 -1.1 1,357,347 + PP_1182 0.33 +0.7 1,357,347 + PP_1182 0.33 +1.9 1,357,348 - PP_1182 0.33 +0.0 1,357,348 - PP_1182 0.33 +0.4 1,357,493 - PP_1182 0.42 -0.2 1,357,570 + PP_1182 0.47 +0.6 1,357,570 + PP_1182 0.47 +0.4
Or see this region's nucleotide sequence