Experiment: MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0422 and PP_0423 are separated by 147 nucleotides PP_0423 and PP_0424 overlap by 4 nucleotides PP_0424 and PP_0425 are separated by 190 nucleotides PP_0425 and PP_0426 are separated by 76 nucleotides
PP_0422: PP_0422 - Indole-3-glycerol phosphate synthase, at 511,027 to 511,860
_0422
PP_0423: PP_0423 - conserved protein of unknown function, at 512,008 to 512,712
_0423
PP_0424: PP_0424 - DNA-binding transcriptional dual regulator, at 512,709 to 513,353
_0424
PP_0425: PP_0425 - conserved protein of unknown function, at 513,544 to 513,966
_0425
PP_0426: PP_0426 - conserved membrane protein of unknown function, at 514,043 to 515,083
_0426
Position (kb) 512
513
514 Strain fitness (log2 ratio) -2
-1
0
1
2 at 511.902 kb on - strand at 511.931 kb on + strand at 511.931 kb on + strand at 511.997 kb on + strand at 511.997 kb on + strand at 511.997 kb on + strand at 511.998 kb on - strand at 511.998 kb on - strand at 512.425 kb on + strand, within PP_0423 at 512.645 kb on + strand at 512.677 kb on + strand at 512.687 kb on + strand at 512.687 kb on + strand at 512.688 kb on - strand at 512.688 kb on - strand at 512.688 kb on - strand at 512.710 kb on + strand at 512.711 kb on - strand at 512.957 kb on + strand, within PP_0424 at 512.998 kb on + strand, within PP_0424 at 513.043 kb on + strand at 513.043 kb on + strand, within PP_0424 at 513.043 kb on + strand, within PP_0424 at 513.129 kb on + strand, within PP_0424 at 513.130 kb on - strand, within PP_0424 at 513.130 kb on - strand, within PP_0424 at 513.263 kb on + strand, within PP_0424 at 513.264 kb on - strand, within PP_0424 at 513.280 kb on + strand, within PP_0424 at 513.300 kb on + strand at 513.300 kb on + strand at 513.301 kb on - strand at 513.301 kb on - strand at 513.371 kb on + strand at 513.380 kb on + strand at 513.381 kb on - strand at 513.433 kb on + strand at 513.434 kb on - strand at 513.444 kb on - strand at 513.459 kb on - strand at 513.461 kb on + strand at 513.504 kb on + strand at 513.504 kb on + strand at 513.504 kb on + strand at 513.505 kb on - strand at 513.505 kb on - strand at 513.505 kb on - strand at 513.556 kb on - strand at 513.556 kb on - strand at 513.556 kb on - strand at 513.792 kb on + strand, within PP_0425 at 513.793 kb on - strand, within PP_0425 at 513.793 kb on - strand, within PP_0425 at 513.889 kb on + strand, within PP_0425 at 513.890 kb on - strand, within PP_0425 at 513.933 kb on + strand at 513.933 kb on + strand at 513.934 kb on - strand at 513.943 kb on + strand at 513.944 kb on - strand at 513.953 kb on + strand at 513.953 kb on + strand at 513.953 kb on + strand at 514.150 kb on + strand, within PP_0426 at 514.151 kb on - strand, within PP_0426 at 514.212 kb on + strand, within PP_0426 at 514.213 kb on - strand, within PP_0426 at 514.213 kb on - strand, within PP_0426 at 514.213 kb on - strand, within PP_0426 at 514.213 kb on - strand, within PP_0426 at 514.221 kb on + strand, within PP_0426 at 514.231 kb on - strand, within PP_0426 at 514.320 kb on + strand, within PP_0426 at 514.320 kb on + strand, within PP_0426 at 514.320 kb on + strand, within PP_0426 at 514.320 kb on + strand, within PP_0426 at 514.320 kb on + strand, within PP_0426 at 514.321 kb on - strand, within PP_0426 at 514.321 kb on - strand, within PP_0426 at 514.321 kb on - strand, within PP_0426
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL remove 511,902 - +0.8 511,931 + -0.0 511,931 + +0.6 511,997 + +1.0 511,997 + +0.4 511,997 + +0.3 511,998 - +0.7 511,998 - -0.2 512,425 + PP_0423 0.59 -1.4 512,645 + -0.1 512,677 + -0.0 512,687 + +0.6 512,687 + -2.7 512,688 - -0.8 512,688 - +0.5 512,688 - -1.4 512,710 + +0.7 512,711 - -0.3 512,957 + PP_0424 0.38 +0.6 512,998 + PP_0424 0.45 +1.0 513,043 + +0.4 513,043 + PP_0424 0.52 +0.7 513,043 + PP_0424 0.52 -0.2 513,129 + PP_0424 0.65 -0.2 513,130 - PP_0424 0.65 +1.1 513,130 - PP_0424 0.65 +0.2 513,263 + PP_0424 0.86 +1.0 513,264 - PP_0424 0.86 +0.3 513,280 + PP_0424 0.89 -0.7 513,300 + -1.3 513,300 + -0.2 513,301 - -1.4 513,301 - +0.2 513,371 + -0.3 513,380 + +0.7 513,381 - -0.0 513,433 + -0.3 513,434 - +0.2 513,444 - +0.2 513,459 - +0.3 513,461 + +0.5 513,504 + +0.7 513,504 + +1.3 513,504 + -0.2 513,505 - -0.2 513,505 - -1.0 513,505 - -0.0 513,556 - +0.2 513,556 - +1.2 513,556 - +0.6 513,792 + PP_0425 0.59 +1.2 513,793 - PP_0425 0.59 -0.2 513,793 - PP_0425 0.59 +0.4 513,889 + PP_0425 0.82 -0.3 513,890 - PP_0425 0.82 +0.4 513,933 + -0.5 513,933 + -1.0 513,934 - -0.1 513,943 + -0.3 513,944 - +1.6 513,953 + +0.7 513,953 + -0.4 513,953 + +0.9 514,150 + PP_0426 0.10 -0.6 514,151 - PP_0426 0.10 +1.0 514,212 + PP_0426 0.16 +1.6 514,213 - PP_0426 0.16 +0.0 514,213 - PP_0426 0.16 -0.5 514,213 - PP_0426 0.16 +0.2 514,213 - PP_0426 0.16 +2.2 514,221 + PP_0426 0.17 -0.4 514,231 - PP_0426 0.18 +0.6 514,320 + PP_0426 0.27 +0.5 514,320 + PP_0426 0.27 -0.1 514,320 + PP_0426 0.27 -0.2 514,320 + PP_0426 0.27 -0.3 514,320 + PP_0426 0.27 -0.4 514,321 - PP_0426 0.27 +0.4 514,321 - PP_0426 0.27 +0.4 514,321 - PP_0426 0.27 -0.7
Or see this region's nucleotide sequence