Experiment: MOPS minimal media_Glucose20mM with Lithium chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2506 and PP_2507 are separated by 45 nucleotides PP_2507 and PP_5532 are separated by 171 nucleotides PP_5532 and PP_5533 are separated by 149 nucleotides PP_5533 and PP_2508 are separated by 398 nucleotides PP_2508 and PP_5534 overlap by 4 nucleotides
PP_2506: PP_2506 - conserved membrane protein of unknown function, at 2,853,649 to 2,854,188
_2506
PP_2507: PP_2507 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 2,854,234 to 2,854,950
_2507
PP_5532: PP_5532 - conserved protein of unknown function, at 2,855,122 to 2,855,385
_5532
PP_5533: PP_5533 - conserved protein of unknown function, at 2,855,535 to 2,855,777
_5533
PP_2508: PP_2508 - conserved protein of unknown function, at 2,856,176 to 2,856,343
_2508
PP_5534: PP_5534 - protein of unknown function, at 2,856,340 to 2,856,570
_5534
Position (kb)
2855
2856 Strain fitness (log2 ratio)
-2
-1
0
1 at 2854.166 kb on + strand at 2854.219 kb on + strand at 2854.220 kb on - strand at 2854.250 kb on + strand at 2854.323 kb on - strand, within PP_2507 at 2854.323 kb on - strand, within PP_2507 at 2854.413 kb on - strand, within PP_2507 at 2854.413 kb on - strand, within PP_2507 at 2854.459 kb on + strand, within PP_2507 at 2854.460 kb on - strand, within PP_2507 at 2854.501 kb on + strand, within PP_2507 at 2854.501 kb on + strand, within PP_2507 at 2854.501 kb on + strand, within PP_2507 at 2854.502 kb on - strand, within PP_2507 at 2854.502 kb on - strand, within PP_2507 at 2854.502 kb on - strand, within PP_2507 at 2854.774 kb on + strand, within PP_2507 at 2854.908 kb on - strand at 2855.051 kb on + strand at 2855.087 kb on + strand at 2855.087 kb on + strand at 2855.087 kb on + strand at 2855.088 kb on - strand at 2855.088 kb on - strand at 2855.088 kb on - strand at 2855.088 kb on - strand at 2855.180 kb on + strand, within PP_5532 at 2855.181 kb on - strand, within PP_5532 at 2855.246 kb on + strand, within PP_5532 at 2855.247 kb on - strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.291 kb on - strand, within PP_5532 at 2855.339 kb on + strand, within PP_5532 at 2855.339 kb on + strand at 2855.340 kb on - strand, within PP_5532 at 2855.340 kb on - strand, within PP_5532 at 2855.340 kb on - strand, within PP_5532 at 2855.340 kb on - strand, within PP_5532 at 2855.340 kb on - strand, within PP_5532 at 2855.520 kb on + strand at 2855.520 kb on + strand at 2855.520 kb on + strand at 2855.543 kb on + strand at 2855.578 kb on - strand, within PP_5533 at 2855.783 kb on - strand at 2855.807 kb on + strand at 2855.808 kb on - strand at 2855.808 kb on - strand at 2855.808 kb on - strand at 2855.845 kb on + strand at 2855.903 kb on - strand at 2856.173 kb on + strand at 2856.173 kb on + strand at 2856.173 kb on + strand at 2856.174 kb on - strand at 2856.174 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Lithium chloride 200 mM remove 2,854,166 + -1.7 2,854,219 + -0.1 2,854,220 - -0.2 2,854,250 + +0.1 2,854,323 - PP_2507 0.12 +0.8 2,854,323 - PP_2507 0.12 +0.7 2,854,413 - PP_2507 0.25 -0.6 2,854,413 - PP_2507 0.25 -0.8 2,854,459 + PP_2507 0.31 +1.2 2,854,460 - PP_2507 0.32 -0.5 2,854,501 + PP_2507 0.37 +0.1 2,854,501 + PP_2507 0.37 -0.6 2,854,501 + PP_2507 0.37 -0.4 2,854,502 - PP_2507 0.37 +0.4 2,854,502 - PP_2507 0.37 -0.4 2,854,502 - PP_2507 0.37 +1.0 2,854,774 + PP_2507 0.75 -0.6 2,854,908 - -0.3 2,855,051 + -0.2 2,855,087 + +0.4 2,855,087 + -0.2 2,855,087 + -0.6 2,855,088 - -0.9 2,855,088 - -2.3 2,855,088 - -0.8 2,855,088 - +1.6 2,855,180 + PP_5532 0.22 +0.9 2,855,181 - PP_5532 0.22 +1.6 2,855,246 + PP_5532 0.47 -1.0 2,855,247 - PP_5532 0.47 +0.3 2,855,290 + PP_5532 0.64 +0.6 2,855,290 + PP_5532 0.64 +0.0 2,855,290 + PP_5532 0.64 -0.2 2,855,290 + PP_5532 0.64 +1.1 2,855,290 + PP_5532 0.64 -1.1 2,855,291 - PP_5532 0.64 +1.4 2,855,339 + PP_5532 0.82 +0.1 2,855,339 + +0.6 2,855,340 - PP_5532 0.83 +1.0 2,855,340 - PP_5532 0.83 +0.5 2,855,340 - PP_5532 0.83 +0.3 2,855,340 - PP_5532 0.83 +0.6 2,855,340 - PP_5532 0.83 +0.3 2,855,520 + +1.6 2,855,520 + +0.2 2,855,520 + -1.3 2,855,543 + +1.2 2,855,578 - PP_5533 0.18 -0.5 2,855,783 - +0.5 2,855,807 + -0.6 2,855,808 - +0.3 2,855,808 - -1.5 2,855,808 - +0.9 2,855,845 + +0.2 2,855,903 - +0.2 2,856,173 + -1.6 2,856,173 + -0.0 2,856,173 + +1.1 2,856,174 - -2.0 2,856,174 - -0.6
Or see this region's nucleotide sequence