Experiment: MOPS minimal media_Glucose20mM with Lithium chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3189 and PP_3190 are separated by 13 nucleotides PP_3190 and PP_3191 overlap by 4 nucleotides PP_3191 and PP_3192 are separated by 30 nucleotides PP_3192 and PP_3193 are separated by 225 nucleotides
PP_3189: PP_3189 - cytosine deaminase / isoguanine deaminase, at 3,618,150 to 3,619,391
_3189
PP_3190: PP_3190 - Ornithine cyclodeaminase/mu-crystallin family protein, at 3,619,405 to 3,620,352
_3190
PP_3191: PP_3191 - putative threonine ammonia-lyase / dehydratase, at 3,620,349 to 3,621,401
_3191
PP_3192: PP_3192 - conserved protein of unknown function, at 3,621,432 to 3,622,037
_3192
PP_3193: PP_3193 - conserved protein of unknown function, at 3,622,263 to 3,622,721
_3193
Position (kb)
3620
3621
3622 Strain fitness (log2 ratio)
-1
0
1 at 3619.482 kb on + strand at 3619.482 kb on + strand at 3619.702 kb on - strand, within PP_3190 at 3619.847 kb on + strand, within PP_3190 at 3620.135 kb on + strand, within PP_3190 at 3620.549 kb on + strand, within PP_3191 at 3620.829 kb on + strand, within PP_3191 at 3620.866 kb on + strand, within PP_3191 at 3620.866 kb on + strand, within PP_3191 at 3620.866 kb on + strand, within PP_3191 at 3620.883 kb on + strand, within PP_3191 at 3621.013 kb on + strand, within PP_3191 at 3621.014 kb on - strand, within PP_3191 at 3621.014 kb on - strand, within PP_3191 at 3621.299 kb on + strand at 3621.300 kb on - strand at 3621.300 kb on - strand at 3621.300 kb on - strand at 3621.454 kb on + strand at 3621.455 kb on - strand at 3621.460 kb on + strand at 3621.500 kb on - strand, within PP_3192 at 3621.596 kb on + strand, within PP_3192 at 3621.760 kb on + strand, within PP_3192 at 3621.855 kb on - strand, within PP_3192 at 3621.923 kb on + strand, within PP_3192 at 3621.933 kb on - strand, within PP_3192 at 3621.955 kb on - strand, within PP_3192 at 3621.985 kb on + strand at 3622.043 kb on - strand at 3622.069 kb on - strand at 3622.069 kb on - strand at 3622.087 kb on - strand at 3622.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Lithium chloride 200 mM remove 3,619,482 + -1.3 3,619,482 + +0.2 3,619,702 - PP_3190 0.31 -1.0 3,619,847 + PP_3190 0.47 +1.7 3,620,135 + PP_3190 0.77 -0.3 3,620,549 + PP_3191 0.19 -0.1 3,620,829 + PP_3191 0.46 +1.5 3,620,866 + PP_3191 0.49 +0.0 3,620,866 + PP_3191 0.49 +1.3 3,620,866 + PP_3191 0.49 +1.1 3,620,883 + PP_3191 0.51 +1.5 3,621,013 + PP_3191 0.63 -0.2 3,621,014 - PP_3191 0.63 -0.2 3,621,014 - PP_3191 0.63 -0.1 3,621,299 + -0.0 3,621,300 - -0.2 3,621,300 - +0.4 3,621,300 - +1.0 3,621,454 + -1.1 3,621,455 - +0.0 3,621,460 + -0.1 3,621,500 - PP_3192 0.11 -0.1 3,621,596 + PP_3192 0.27 +0.8 3,621,760 + PP_3192 0.54 +0.1 3,621,855 - PP_3192 0.70 -0.5 3,621,923 + PP_3192 0.81 -0.1 3,621,933 - PP_3192 0.83 +0.3 3,621,955 - PP_3192 0.86 -0.1 3,621,985 + +0.0 3,622,043 - -1.6 3,622,069 - +0.1 3,622,069 - -0.4 3,622,087 - +0.3 3,622,157 - +0.2
Or see this region's nucleotide sequence