Experiment: MOPS minimal media_Glucose20mM with Lithium chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0838 and PP_0839 are separated by 151 nucleotides PP_0839 and PP_0840 are separated by 3 nucleotides PP_0840 and PP_0841 are separated by 179 nucleotides
PP_0838: PP_0838 - inositol monophosphatase / glycerol-2-phosphatase, at 975,320 to 976,138
_0838
PP_0839: PP_0839 - tRNA Cm32/Um32 methyltransferase, at 976,290 to 977,045
_0839
PP_0840: PP_0840 - serine acetyltransferase, at 977,049 to 977,834
_0840
PP_0841: PP_0841 - DNA-binding transcriptional dual regulator 2Fe-2S cluster IscR, at 978,014 to 978,505
_0841
Position (kb) 976
977
978 Strain fitness (log2 ratio) -1
0
1
2 at 976.230 kb on + strand at 976.248 kb on - strand at 976.302 kb on - strand at 976.322 kb on + strand at 976.508 kb on + strand, within PP_0839 at 976.854 kb on + strand, within PP_0839 at 976.941 kb on + strand, within PP_0839 at 976.942 kb on - strand, within PP_0839 at 976.942 kb on - strand, within PP_0839 at 977.072 kb on + strand at 977.073 kb on - strand at 977.083 kb on + strand at 977.084 kb on - strand at 977.298 kb on - strand, within PP_0840 at 977.522 kb on + strand, within PP_0840 at 977.588 kb on + strand, within PP_0840 at 977.622 kb on + strand, within PP_0840 at 977.714 kb on + strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.748 kb on + strand, within PP_0840 at 977.748 kb on + strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 978.009 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Lithium chloride 200 mM remove 976,230 + -0.5 976,248 - -0.8 976,302 - -0.0 976,322 + +0.2 976,508 + PP_0839 0.29 -1.0 976,854 + PP_0839 0.75 +2.4 976,941 + PP_0839 0.86 -1.6 976,942 - PP_0839 0.86 +0.4 976,942 - PP_0839 0.86 +0.1 977,072 + +1.5 977,073 - +0.2 977,083 + -0.4 977,084 - -0.1 977,298 - PP_0840 0.32 +0.3 977,522 + PP_0840 0.60 +0.1 977,588 + PP_0840 0.69 +2.1 977,622 + PP_0840 0.73 +0.5 977,714 + PP_0840 0.85 -0.4 977,715 - PP_0840 0.85 +1.4 977,715 - PP_0840 0.85 -0.1 977,715 - PP_0840 0.85 +2.1 977,715 - PP_0840 0.85 -0.7 977,748 + PP_0840 0.89 +0.8 977,748 + PP_0840 0.89 +1.5 977,749 - PP_0840 0.89 -0.7 977,749 - PP_0840 0.89 -0.3 977,749 - PP_0840 0.89 -0.1 977,749 - PP_0840 0.89 -0.9 978,009 + +1.7
Or see this region's nucleotide sequence