Experiment: MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0690 and PP_0691 are separated by 85 nucleotides PP_0691 and PP_0692 overlap by 4 nucleotides PP_0692 and PP_0693 are separated by 93 nucleotides
PP_0690: PP_0690 - GTPase Obg, at 801,516 to 802,742
_0690
PP_0691: PP_0691 - Glutamate 5-kinase, at 802,828 to 803,946
_0691
PP_0692: PP_0692 - conserved protein of unknown function, at 803,943 to 804,404
_0692
PP_0693: PP_0693 - conserved protein of unknown function, at 804,498 to 807,329
_0693
Position (kb) 802
803
804 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 802.728 kb on + strand at 802.728 kb on + strand at 802.728 kb on + strand at 802.753 kb on + strand at 802.788 kb on + strand at 802.798 kb on - strand at 802.801 kb on + strand at 802.801 kb on + strand at 802.802 kb on - strand at 802.802 kb on - strand at 802.814 kb on + strand at 802.814 kb on + strand at 802.866 kb on + strand at 802.976 kb on + strand, within PP_0691 at 803.358 kb on + strand, within PP_0691 at 803.359 kb on - strand, within PP_0691 at 803.359 kb on - strand, within PP_0691 at 803.359 kb on - strand, within PP_0691 at 803.359 kb on - strand at 803.405 kb on - strand, within PP_0691 at 803.830 kb on + strand, within PP_0691 at 803.831 kb on - strand, within PP_0691 at 803.831 kb on - strand, within PP_0691 at 803.896 kb on + strand at 803.896 kb on + strand at 803.897 kb on - strand at 803.899 kb on - strand at 803.918 kb on + strand at 803.942 kb on + strand at 803.962 kb on + strand at 803.962 kb on + strand at 803.962 kb on + strand at 804.087 kb on - strand, within PP_0692 at 804.109 kb on - strand, within PP_0692 at 804.311 kb on + strand, within PP_0692 at 804.311 kb on + strand, within PP_0692 at 804.311 kb on + strand, within PP_0692 at 804.312 kb on - strand, within PP_0692 at 804.312 kb on - strand, within PP_0692 at 804.321 kb on + strand, within PP_0692 at 804.321 kb on + strand, within PP_0692 at 804.322 kb on - strand, within PP_0692 at 804.330 kb on + strand, within PP_0692 at 804.405 kb on + strand at 804.406 kb on - strand at 804.406 kb on - strand at 804.420 kb on + strand at 804.421 kb on - strand at 804.421 kb on - strand at 804.421 kb on - strand at 804.451 kb on - strand at 804.451 kb on - strand at 804.451 kb on - strand at 804.586 kb on + strand at 804.586 kb on + strand at 804.586 kb on + strand at 804.587 kb on - strand at 804.587 kb on - strand at 804.745 kb on + strand at 804.746 kb on - strand at 804.746 kb on - strand at 804.748 kb on + strand at 804.802 kb on + strand, within PP_0693 at 804.802 kb on + strand, within PP_0693 at 804.802 kb on + strand, within PP_0693 at 804.802 kb on + strand, within PP_0693 at 804.802 kb on + strand, within PP_0693 at 804.803 kb on - strand, within PP_0693 at 804.803 kb on - strand, within PP_0693 at 804.803 kb on - strand, within PP_0693
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM remove 802,728 + +1.9 802,728 + +0.2 802,728 + +0.4 802,753 + +0.2 802,788 + +0.3 802,798 - -4.8 802,801 + +0.4 802,801 + -0.3 802,802 - -5.8 802,802 - -6.2 802,814 + -1.8 802,814 + -3.1 802,866 + -5.8 802,976 + PP_0691 0.13 -6.2 803,358 + PP_0691 0.47 -5.2 803,359 - PP_0691 0.47 -6.0 803,359 - PP_0691 0.47 -6.5 803,359 - PP_0691 0.47 -5.1 803,359 - -2.8 803,405 - PP_0691 0.52 -5.1 803,830 + PP_0691 0.90 -5.5 803,831 - PP_0691 0.90 -6.0 803,831 - PP_0691 0.90 -5.4 803,896 + -3.6 803,896 + -6.0 803,897 - -4.1 803,899 - -4.4 803,918 + -7.0 803,942 + -0.7 803,962 + +0.3 803,962 + +0.1 803,962 + +0.3 804,087 - PP_0692 0.31 +0.3 804,109 - PP_0692 0.36 -0.3 804,311 + PP_0692 0.80 -1.1 804,311 + PP_0692 0.80 +0.1 804,311 + PP_0692 0.80 -0.8 804,312 - PP_0692 0.80 +0.8 804,312 - PP_0692 0.80 +0.2 804,321 + PP_0692 0.82 -0.7 804,321 + PP_0692 0.82 -0.8 804,322 - PP_0692 0.82 +0.2 804,330 + PP_0692 0.84 +0.1 804,405 + -0.4 804,406 - -0.0 804,406 - -0.1 804,420 + +0.6 804,421 - +1.9 804,421 - -1.4 804,421 - -0.2 804,451 - -0.1 804,451 - -0.0 804,451 - +0.1 804,586 + -0.3 804,586 + -0.2 804,586 + -0.7 804,587 - -0.3 804,587 - -0.4 804,745 + -2.0 804,746 - -0.3 804,746 - +0.2 804,748 + -0.4 804,802 + PP_0693 0.11 +0.8 804,802 + PP_0693 0.11 -0.5 804,802 + PP_0693 0.11 +0.2 804,802 + PP_0693 0.11 +0.4 804,802 + PP_0693 0.11 +0.6 804,803 - PP_0693 0.11 -0.3 804,803 - PP_0693 0.11 -0.4 804,803 - PP_0693 0.11 -0.7
Or see this region's nucleotide sequence