Experiment: MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1964 and PP_1966 are separated by 446 nucleotides PP_1966 and PP_1967 are separated by 30 nucleotides
PP_1964: PP_1964 - putative dGMP, dTMP, dhydroxyCMP kinase, at 2,226,598 to 2,227,401
_1964
PP_1966: PP_1966 - DNA polymerase III subunit delta', at 2,227,848 to 2,228,834
_1966
PP_1967: PP_1967 - tRNA D-aminoacylase, at 2,228,865 to 2,229,647
_1967
Position (kb)
2227
2228
2229 Strain fitness (log2 ratio)
-2
-1
0
1 at 2227.443 kb on - strand at 2227.512 kb on + strand at 2227.526 kb on + strand at 2227.730 kb on + strand at 2227.730 kb on + strand at 2227.763 kb on + strand at 2227.818 kb on + strand at 2227.818 kb on + strand at 2228.860 kb on - strand at 2228.872 kb on + strand at 2228.872 kb on + strand at 2229.030 kb on + strand, within PP_1967 at 2229.030 kb on + strand, within PP_1967 at 2229.257 kb on + strand, within PP_1967 at 2229.257 kb on + strand, within PP_1967 at 2229.336 kb on - strand, within PP_1967 at 2229.378 kb on + strand, within PP_1967 at 2229.378 kb on + strand, within PP_1967 at 2229.381 kb on + strand, within PP_1967 at 2229.381 kb on + strand, within PP_1967 at 2229.381 kb on + strand, within PP_1967 at 2229.382 kb on - strand, within PP_1967 at 2229.531 kb on + strand, within PP_1967 at 2229.532 kb on - strand, within PP_1967 at 2229.552 kb on + strand, within PP_1967 at 2229.552 kb on + strand, within PP_1967 at 2229.553 kb on - strand, within PP_1967 at 2229.553 kb on - strand, within PP_1967 at 2229.579 kb on + strand at 2229.579 kb on + strand at 2229.579 kb on + strand at 2229.579 kb on + strand at 2229.754 kb on - strand at 2229.769 kb on - strand at 2229.779 kb on + strand at 2229.779 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM remove 2,227,443 - +0.3 2,227,512 + -1.0 2,227,526 + +0.6 2,227,730 + -0.0 2,227,730 + +0.0 2,227,763 + +0.3 2,227,818 + -0.4 2,227,818 + +1.7 2,228,860 - -0.1 2,228,872 + -1.6 2,228,872 + -0.6 2,229,030 + PP_1967 0.21 +0.1 2,229,030 + PP_1967 0.21 -0.7 2,229,257 + PP_1967 0.50 -0.7 2,229,257 + PP_1967 0.50 -0.3 2,229,336 - PP_1967 0.60 -0.5 2,229,378 + PP_1967 0.66 +0.3 2,229,378 + PP_1967 0.66 +1.2 2,229,381 + PP_1967 0.66 +0.9 2,229,381 + PP_1967 0.66 +0.8 2,229,381 + PP_1967 0.66 -1.5 2,229,382 - PP_1967 0.66 +0.4 2,229,531 + PP_1967 0.85 -2.0 2,229,532 - PP_1967 0.85 -0.2 2,229,552 + PP_1967 0.88 +0.6 2,229,552 + PP_1967 0.88 -0.2 2,229,553 - PP_1967 0.88 -0.0 2,229,553 - PP_1967 0.88 -1.1 2,229,579 + -2.1 2,229,579 + +0.5 2,229,579 + +0.2 2,229,579 + -1.3 2,229,754 - +1.0 2,229,769 - -0.2 2,229,779 + +0.4 2,229,779 + +0.7
Or see this region's nucleotide sequence