Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4768 and PP_4769 are separated by 9 nucleotides PP_4769 and PP_4770 are separated by 160 nucleotides PP_4770 and PP_4771 are separated by 167 nucleotides PP_4771 and PP_5713 are separated by 85 nucleotides
PP_4768: PP_4768 - Exonuclease, at 5,429,218 to 5,429,829
_4768
PP_4769: PP_4769 - conserved protein of unknown function, at 5,429,839 to 5,430,318
_4769
PP_4770: PP_4770 - conserved protein of unknown function, at 5,430,479 to 5,431,237
_4770
PP_4771: PP_4771 - conserved membrane protein of unknown function, at 5,431,405 to 5,432,061
_4771
PP_5713: PP_5713 - conserved protein of unknown function, at 5,432,147 to 5,432,665
_5713
Position (kb) 5430
5431
5432 Strain fitness (log2 ratio) -1
0
1
2
3 at 5429.531 kb on + strand, within PP_4768 at 5429.532 kb on - strand, within PP_4768 at 5429.790 kb on + strand at 5429.791 kb on - strand at 5429.820 kb on + strand at 5429.820 kb on + strand at 5429.820 kb on + strand at 5429.821 kb on - strand at 5429.821 kb on - strand at 5429.840 kb on + strand at 5429.876 kb on + strand at 5429.877 kb on - strand at 5429.927 kb on + strand, within PP_4769 at 5429.927 kb on + strand at 5429.928 kb on - strand, within PP_4769 at 5429.928 kb on - strand, within PP_4769 at 5430.048 kb on + strand, within PP_4769 at 5430.182 kb on + strand, within PP_4769 at 5430.240 kb on - strand, within PP_4769 at 5430.275 kb on + strand at 5430.275 kb on + strand at 5430.276 kb on - strand at 5430.276 kb on - strand at 5430.278 kb on + strand at 5430.278 kb on + strand at 5430.279 kb on - strand at 5430.279 kb on - strand at 5430.279 kb on - strand at 5430.279 kb on - strand at 5430.466 kb on + strand at 5430.466 kb on + strand at 5430.486 kb on + strand at 5430.487 kb on - strand at 5430.487 kb on - strand at 5430.544 kb on - strand at 5430.616 kb on + strand, within PP_4770 at 5430.617 kb on - strand, within PP_4770 at 5430.869 kb on + strand, within PP_4770 at 5430.870 kb on - strand, within PP_4770 at 5430.870 kb on - strand, within PP_4770 at 5431.098 kb on + strand, within PP_4770 at 5431.098 kb on + strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.099 kb on - strand, within PP_4770 at 5431.101 kb on + strand, within PP_4770 at 5431.102 kb on - strand, within PP_4770 at 5431.102 kb on - strand, within PP_4770 at 5431.289 kb on + strand at 5431.289 kb on + strand at 5431.369 kb on + strand at 5431.369 kb on + strand at 5431.370 kb on - strand at 5431.438 kb on + strand at 5431.438 kb on + strand at 5431.439 kb on - strand at 5431.439 kb on - strand at 5431.591 kb on + strand, within PP_4771 at 5431.591 kb on + strand, within PP_4771 at 5431.592 kb on - strand, within PP_4771 at 5431.612 kb on + strand, within PP_4771 at 5431.703 kb on + strand, within PP_4771 at 5431.703 kb on + strand, within PP_4771 at 5431.792 kb on + strand, within PP_4771 at 5431.889 kb on + strand, within PP_4771 at 5432.130 kb on + strand at 5432.130 kb on + strand at 5432.131 kb on - strand at 5432.198 kb on + strand at 5432.198 kb on + strand at 5432.198 kb on + strand at 5432.199 kb on - strand, within PP_5713 at 5432.199 kb on - strand, within PP_5713 at 5432.199 kb on - strand, within PP_5713
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 5,429,531 + PP_4768 0.51 +1.6 5,429,532 - PP_4768 0.51 +0.6 5,429,790 + +0.3 5,429,791 - +0.2 5,429,820 + -1.6 5,429,820 + -0.6 5,429,820 + +0.4 5,429,821 - +0.0 5,429,821 - +1.2 5,429,840 + -0.6 5,429,876 + +0.9 5,429,877 - -1.3 5,429,927 + PP_4769 0.18 +0.2 5,429,927 + +0.9 5,429,928 - PP_4769 0.19 +1.4 5,429,928 - PP_4769 0.19 +0.6 5,430,048 + PP_4769 0.44 -0.3 5,430,182 + PP_4769 0.71 -0.1 5,430,240 - PP_4769 0.84 +0.7 5,430,275 + -0.5 5,430,275 + +0.2 5,430,276 - -1.0 5,430,276 - +0.7 5,430,278 + +1.6 5,430,278 + +0.1 5,430,279 - +0.8 5,430,279 - +0.4 5,430,279 - +0.4 5,430,279 - +0.3 5,430,466 + -0.0 5,430,466 + -0.1 5,430,486 + +0.5 5,430,487 - -0.4 5,430,487 - -0.0 5,430,544 - +0.6 5,430,616 + PP_4770 0.18 -1.2 5,430,617 - PP_4770 0.18 +0.5 5,430,869 + PP_4770 0.51 +0.6 5,430,870 - PP_4770 0.52 +0.2 5,430,870 - PP_4770 0.52 -0.2 5,431,098 + PP_4770 0.82 +1.2 5,431,098 + PP_4770 0.82 -0.5 5,431,099 - PP_4770 0.82 +2.3 5,431,099 - PP_4770 0.82 -1.0 5,431,099 - PP_4770 0.82 +0.9 5,431,099 - PP_4770 0.82 +0.4 5,431,099 - PP_4770 0.82 -0.6 5,431,099 - PP_4770 0.82 +0.1 5,431,099 - PP_4770 0.82 -0.4 5,431,101 + PP_4770 0.82 +0.3 5,431,102 - PP_4770 0.82 +3.4 5,431,102 - PP_4770 0.82 -0.7 5,431,289 + +0.5 5,431,289 + -1.0 5,431,369 + +2.3 5,431,369 + -0.1 5,431,370 - -1.1 5,431,438 + -1.2 5,431,438 + +1.1 5,431,439 - -0.1 5,431,439 - -0.1 5,431,591 + PP_4771 0.28 +0.3 5,431,591 + PP_4771 0.28 +0.6 5,431,592 - PP_4771 0.28 +0.5 5,431,612 + PP_4771 0.32 +0.0 5,431,703 + PP_4771 0.45 +0.1 5,431,703 + PP_4771 0.45 +0.2 5,431,792 + PP_4771 0.59 +1.5 5,431,889 + PP_4771 0.74 +0.8 5,432,130 + -0.3 5,432,130 + -0.1 5,432,131 - -0.8 5,432,198 + +0.2 5,432,198 + -0.0 5,432,198 + +0.5 5,432,199 - PP_5713 0.10 +0.3 5,432,199 - PP_5713 0.10 -0.8 5,432,199 - PP_5713 0.10 +0.5
Or see this region's nucleotide sequence