Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2819 and PP_2820 are separated by 82 nucleotides PP_2820 and PP_2821 are separated by 177 nucleotides PP_2821 and PP_5539 are separated by 11 nucleotides
PP_2819: PP_2819 - Outer membrane protein OprJ, at 3,217,211 to 3,218,617
_2819
PP_2820: PP_2820 - HTH-type transcriptional regulator nfxB, at 3,218,700 to 3,219,257
_2820
PP_2821: PP_2821 - conserved exported protein of unknown function, at 3,219,435 to 3,219,839
_2821
PP_5539: PP_5539 - conserved exported protein of unknown function, at 3,219,851 to 3,221,011
_5539
Position (kb)
3219
3220 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3218.463 kb on - strand, within PP_2819 at 3218.493 kb on - strand at 3218.516 kb on + strand at 3218.516 kb on + strand at 3218.516 kb on + strand at 3218.516 kb on + strand at 3218.517 kb on - strand at 3218.803 kb on + strand, within PP_2820 at 3218.804 kb on - strand, within PP_2820 at 3218.804 kb on - strand, within PP_2820 at 3218.804 kb on - strand, within PP_2820 at 3219.092 kb on - strand, within PP_2820 at 3219.277 kb on + strand at 3219.311 kb on - strand at 3219.331 kb on + strand at 3219.488 kb on + strand, within PP_2821 at 3219.490 kb on + strand, within PP_2821 at 3219.490 kb on + strand, within PP_2821 at 3219.491 kb on - strand, within PP_2821 at 3219.596 kb on - strand, within PP_2821 at 3219.638 kb on - strand, within PP_2821 at 3219.708 kb on + strand, within PP_2821 at 3219.708 kb on + strand, within PP_2821 at 3219.708 kb on + strand, within PP_2821 at 3219.709 kb on - strand, within PP_2821 at 3219.709 kb on - strand, within PP_2821 at 3219.852 kb on + strand at 3219.852 kb on + strand at 3219.894 kb on + strand at 3219.894 kb on + strand at 3219.895 kb on - strand at 3219.895 kb on - strand at 3220.053 kb on + strand, within PP_5539 at 3220.053 kb on + strand, within PP_5539 at 3220.065 kb on + strand, within PP_5539 at 3220.110 kb on + strand, within PP_5539 at 3220.110 kb on + strand, within PP_5539 at 3220.111 kb on - strand, within PP_5539 at 3220.114 kb on + strand, within PP_5539 at 3220.115 kb on - strand, within PP_5539 at 3220.115 kb on - strand, within PP_5539 at 3220.158 kb on + strand, within PP_5539 at 3220.159 kb on - strand, within PP_5539 at 3220.159 kb on - strand, within PP_5539 at 3220.159 kb on - strand, within PP_5539 at 3220.243 kb on - strand, within PP_5539 at 3220.395 kb on + strand, within PP_5539 at 3220.446 kb on + strand, within PP_5539 at 3220.536 kb on + strand, within PP_5539 at 3220.536 kb on + strand, within PP_5539 at 3220.536 kb on + strand, within PP_5539 at 3220.557 kb on + strand, within PP_5539 at 3220.558 kb on - strand, within PP_5539 at 3220.581 kb on + strand, within PP_5539 at 3220.641 kb on + strand, within PP_5539 at 3220.641 kb on + strand, within PP_5539 at 3220.647 kb on + strand, within PP_5539 at 3220.648 kb on - strand, within PP_5539 at 3220.648 kb on - strand, within PP_5539 at 3220.648 kb on - strand, within PP_5539 at 3220.648 kb on - strand, within PP_5539 at 3220.710 kb on + strand, within PP_5539 at 3220.710 kb on + strand, within PP_5539 at 3220.710 kb on + strand, within PP_5539 at 3220.711 kb on - strand, within PP_5539
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 3,218,463 - PP_2819 0.89 +1.3 3,218,493 - +1.0 3,218,516 + -0.9 3,218,516 + +0.2 3,218,516 + +0.3 3,218,516 + +0.9 3,218,517 - -0.4 3,218,803 + PP_2820 0.18 -1.4 3,218,804 - PP_2820 0.19 -1.6 3,218,804 - PP_2820 0.19 -0.4 3,218,804 - PP_2820 0.19 +0.0 3,219,092 - PP_2820 0.70 +0.0 3,219,277 + +0.0 3,219,311 - +1.1 3,219,331 + -0.9 3,219,488 + PP_2821 0.13 -0.5 3,219,490 + PP_2821 0.14 +1.2 3,219,490 + PP_2821 0.14 +2.6 3,219,491 - PP_2821 0.14 +0.1 3,219,596 - PP_2821 0.40 +0.0 3,219,638 - PP_2821 0.50 +1.2 3,219,708 + PP_2821 0.67 +0.6 3,219,708 + PP_2821 0.67 +0.5 3,219,708 + PP_2821 0.67 +0.2 3,219,709 - PP_2821 0.68 +1.6 3,219,709 - PP_2821 0.68 +0.1 3,219,852 + +1.1 3,219,852 + +0.1 3,219,894 + -0.9 3,219,894 + +0.4 3,219,895 - +0.3 3,219,895 - -0.8 3,220,053 + PP_5539 0.17 -0.4 3,220,053 + PP_5539 0.17 +0.3 3,220,065 + PP_5539 0.18 -1.2 3,220,110 + PP_5539 0.22 +0.2 3,220,110 + PP_5539 0.22 -0.6 3,220,111 - PP_5539 0.22 +0.3 3,220,114 + PP_5539 0.23 -0.3 3,220,115 - PP_5539 0.23 +0.4 3,220,115 - PP_5539 0.23 -0.1 3,220,158 + PP_5539 0.26 -0.6 3,220,159 - PP_5539 0.27 +0.5 3,220,159 - PP_5539 0.27 +0.4 3,220,159 - PP_5539 0.27 +0.0 3,220,243 - PP_5539 0.34 +0.7 3,220,395 + PP_5539 0.47 +0.6 3,220,446 + PP_5539 0.51 +0.2 3,220,536 + PP_5539 0.59 +0.8 3,220,536 + PP_5539 0.59 -1.0 3,220,536 + PP_5539 0.59 +0.2 3,220,557 + PP_5539 0.61 +0.6 3,220,558 - PP_5539 0.61 +0.2 3,220,581 + PP_5539 0.63 -0.9 3,220,641 + PP_5539 0.68 -0.6 3,220,641 + PP_5539 0.68 -2.0 3,220,647 + PP_5539 0.69 +0.6 3,220,648 - PP_5539 0.69 +0.4 3,220,648 - PP_5539 0.69 -1.6 3,220,648 - PP_5539 0.69 +0.1 3,220,648 - PP_5539 0.69 +0.1 3,220,710 + PP_5539 0.74 -0.4 3,220,710 + PP_5539 0.74 +2.3 3,220,710 + PP_5539 0.74 +0.0 3,220,711 - PP_5539 0.74 +1.6
Or see this region's nucleotide sequence