Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0825 and PP_0826 overlap by 7 nucleotides PP_0826 and PP_0827 overlap by 4 nucleotides PP_0827 and PP_0828 are separated by 36 nucleotides
PP_0825: PP_0825 - phosphonates import ATP-binding protein, at 963,392 to 964,201
_0825
PP_0826: PP_0826 - phosphonate ABC transporter, permease protein, at 964,195 to 965,022
_0826
PP_0827: PP_0827 - putative phosphonate transport system permease protein PtxC, at 965,019 to 965,786
_0827
PP_0828: PP_0828 - conserved membrane protein of unknown function, at 965,823 to 966,716
_0828
Position (kb) 965
966 Strain fitness (log2 ratio) -2
-1
0
1 at 964.023 kb on + strand, within PP_0825 at 964.265 kb on + strand at 964.266 kb on - strand at 964.399 kb on + strand, within PP_0826 at 964.400 kb on - strand, within PP_0826 at 964.407 kb on + strand, within PP_0826 at 964.587 kb on + strand, within PP_0826 at 964.588 kb on - strand, within PP_0826 at 964.588 kb on - strand, within PP_0826 at 964.655 kb on + strand, within PP_0826 at 964.656 kb on - strand, within PP_0826 at 964.675 kb on + strand at 964.675 kb on + strand, within PP_0826 at 964.675 kb on + strand, within PP_0826 at 964.675 kb on + strand, within PP_0826 at 964.676 kb on - strand, within PP_0826 at 964.676 kb on - strand, within PP_0826 at 964.700 kb on - strand, within PP_0826 at 964.784 kb on - strand, within PP_0826 at 964.822 kb on + strand, within PP_0826 at 964.822 kb on + strand, within PP_0826 at 964.823 kb on - strand, within PP_0826 at 964.831 kb on + strand, within PP_0826 at 965.085 kb on + strand at 965.580 kb on - strand, within PP_0827 at 965.664 kb on + strand, within PP_0827 at 965.664 kb on + strand, within PP_0827 at 965.664 kb on + strand, within PP_0827 at 965.717 kb on + strand at 965.717 kb on + strand at 965.896 kb on - strand at 965.900 kb on + strand at 965.900 kb on + strand at 965.900 kb on + strand at 965.901 kb on - strand at 965.901 kb on - strand at 965.901 kb on - strand at 965.901 kb on - strand at 966.083 kb on - strand, within PP_0828 at 966.083 kb on - strand, within PP_0828 at 966.088 kb on + strand, within PP_0828 at 966.088 kb on + strand, within PP_0828 at 966.088 kb on + strand, within PP_0828 at 966.089 kb on - strand, within PP_0828 at 966.089 kb on - strand, within PP_0828 at 966.089 kb on - strand, within PP_0828 at 966.107 kb on + strand, within PP_0828 at 966.150 kb on + strand, within PP_0828 at 966.151 kb on - strand, within PP_0828 at 966.151 kb on - strand, within PP_0828 at 966.206 kb on - strand, within PP_0828 at 966.358 kb on + strand, within PP_0828 at 966.366 kb on - strand, within PP_0828 at 966.664 kb on + strand at 966.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 964,023 + PP_0825 0.78 +0.3 964,265 + +1.0 964,266 - +0.4 964,399 + PP_0826 0.25 -0.6 964,400 - PP_0826 0.25 +0.6 964,407 + PP_0826 0.26 +0.0 964,587 + PP_0826 0.47 -0.5 964,588 - PP_0826 0.47 +0.5 964,588 - PP_0826 0.47 -0.3 964,655 + PP_0826 0.56 -0.9 964,656 - PP_0826 0.56 +0.1 964,675 + +1.4 964,675 + PP_0826 0.58 -0.4 964,675 + PP_0826 0.58 +1.0 964,675 + PP_0826 0.58 +0.5 964,676 - PP_0826 0.58 +0.2 964,676 - PP_0826 0.58 +0.4 964,700 - PP_0826 0.61 -0.0 964,784 - PP_0826 0.71 -0.6 964,822 + PP_0826 0.76 +0.7 964,822 + PP_0826 0.76 -0.3 964,823 - PP_0826 0.76 +1.1 964,831 + PP_0826 0.77 +0.2 965,085 + +0.6 965,580 - PP_0827 0.73 +0.9 965,664 + PP_0827 0.84 +0.2 965,664 + PP_0827 0.84 +1.1 965,664 + PP_0827 0.84 +0.0 965,717 + -0.2 965,717 + -1.5 965,896 - -0.6 965,900 + -0.0 965,900 + +0.8 965,900 + -1.7 965,901 - -0.9 965,901 - +0.1 965,901 - -0.7 965,901 - +0.4 966,083 - PP_0828 0.29 -0.6 966,083 - PP_0828 0.29 +0.0 966,088 + PP_0828 0.30 -0.0 966,088 + PP_0828 0.30 -0.4 966,088 + PP_0828 0.30 -1.1 966,089 - PP_0828 0.30 -1.9 966,089 - PP_0828 0.30 +0.6 966,089 - PP_0828 0.30 -0.2 966,107 + PP_0828 0.32 +1.4 966,150 + PP_0828 0.37 +0.7 966,151 - PP_0828 0.37 +0.2 966,151 - PP_0828 0.37 -0.2 966,206 - PP_0828 0.43 -0.7 966,358 + PP_0828 0.60 -0.8 966,366 - PP_0828 0.61 +1.6 966,664 + +1.6 966,665 - +0.8
Or see this region's nucleotide sequence