Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0149 and PP_0150 overlap by 4 nucleotides PP_0150 and PP_0151 are separated by 36 nucleotides PP_0151 and PP_0152 are separated by 89 nucleotides PP_0152 and PP_0153 are separated by 245 nucleotides
PP_0149: PP_0149 - conserved exported protein of unknown function, at 158,433 to 160,388
_0149
PP_0150: PP_0150 - conserved exported protein of unknown function, at 160,385 to 160,705
_0150
PP_0151: PP_0151 - conserved exported protein of unknown function, at 160,742 to 161,035
_0151
PP_0152: PP_0152 - conserved exported protein of unknown function, at 161,125 to 161,505
_0152
PP_0153: PP_0153 - hypothetical protein, at 161,751 to 161,966
_0153
Position (kb) 160
161
162 Strain fitness (log2 ratio) -2
-1
0
1
2 at 159.742 kb on + strand, within PP_0149 at 159.743 kb on - strand, within PP_0149 at 159.743 kb on - strand, within PP_0149 at 159.766 kb on + strand, within PP_0149 at 159.766 kb on + strand, within PP_0149 at 159.766 kb on + strand, within PP_0149 at 159.766 kb on + strand, within PP_0149 at 159.767 kb on - strand, within PP_0149 at 159.767 kb on - strand, within PP_0149 at 159.767 kb on - strand, within PP_0149 at 159.767 kb on - strand, within PP_0149 at 159.767 kb on - strand, within PP_0149 at 159.829 kb on + strand, within PP_0149 at 159.829 kb on + strand, within PP_0149 at 159.829 kb on + strand, within PP_0149 at 159.829 kb on + strand, within PP_0149 at 159.830 kb on - strand, within PP_0149 at 159.830 kb on - strand, within PP_0149 at 159.830 kb on - strand, within PP_0149 at 159.830 kb on - strand, within PP_0149 at 160.003 kb on + strand, within PP_0149 at 160.004 kb on - strand, within PP_0149 at 160.140 kb on + strand, within PP_0149 at 160.140 kb on + strand, within PP_0149 at 160.159 kb on + strand, within PP_0149 at 160.274 kb on + strand at 160.275 kb on - strand at 160.397 kb on + strand at 160.413 kb on - strand at 160.459 kb on + strand, within PP_0150 at 160.460 kb on - strand, within PP_0150 at 160.460 kb on - strand, within PP_0150 at 160.481 kb on + strand, within PP_0150 at 160.739 kb on + strand at 160.739 kb on + strand at 160.740 kb on - strand at 160.740 kb on - strand at 160.740 kb on - strand at 160.740 kb on - strand at 160.740 kb on - strand at 160.743 kb on + strand at 160.743 kb on + strand at 160.744 kb on - strand at 160.744 kb on - strand at 160.808 kb on + strand, within PP_0151 at 160.809 kb on - strand, within PP_0151 at 160.894 kb on + strand, within PP_0151 at 160.894 kb on + strand, within PP_0151 at 160.894 kb on + strand, within PP_0151 at 160.941 kb on + strand, within PP_0151 at 160.942 kb on - strand, within PP_0151 at 161.029 kb on - strand at 161.351 kb on - strand, within PP_0152 at 161.433 kb on + strand, within PP_0152 at 161.434 kb on - strand, within PP_0152 at 161.481 kb on - strand at 161.529 kb on + strand at 161.568 kb on + strand at 161.858 kb on + strand, within PP_0153 at 161.858 kb on + strand, within PP_0153 at 161.859 kb on - strand, within PP_0153 at 161.957 kb on + strand at 161.958 kb on - strand at 161.958 kb on - strand at 161.997 kb on + strand at 161.998 kb on - strand at 162.001 kb on + strand at 162.001 kb on + strand at 162.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 159,742 + PP_0149 0.67 -1.2 159,743 - PP_0149 0.67 +0.5 159,743 - PP_0149 0.67 -2.0 159,766 + PP_0149 0.68 -0.2 159,766 + PP_0149 0.68 +0.9 159,766 + PP_0149 0.68 -0.5 159,766 + PP_0149 0.68 +0.3 159,767 - PP_0149 0.68 -0.3 159,767 - PP_0149 0.68 +0.1 159,767 - PP_0149 0.68 +0.9 159,767 - PP_0149 0.68 +0.5 159,767 - PP_0149 0.68 +0.5 159,829 + PP_0149 0.71 -0.4 159,829 + PP_0149 0.71 +1.2 159,829 + PP_0149 0.71 +0.7 159,829 + PP_0149 0.71 -0.9 159,830 - PP_0149 0.71 -1.8 159,830 - PP_0149 0.71 +0.2 159,830 - PP_0149 0.71 +0.6 159,830 - PP_0149 0.71 +0.6 160,003 + PP_0149 0.80 -0.2 160,004 - PP_0149 0.80 +1.1 160,140 + PP_0149 0.87 -2.3 160,140 + PP_0149 0.87 +0.1 160,159 + PP_0149 0.88 -0.1 160,274 + +1.7 160,275 - +1.7 160,397 + +0.2 160,413 - +0.4 160,459 + PP_0150 0.23 -0.3 160,460 - PP_0150 0.23 -0.2 160,460 - PP_0150 0.23 +0.2 160,481 + PP_0150 0.30 -1.2 160,739 + -1.1 160,739 + -0.2 160,740 - -0.9 160,740 - +0.6 160,740 - -0.1 160,740 - -0.9 160,740 - +1.1 160,743 + +0.5 160,743 + +1.9 160,744 - -0.3 160,744 - -0.0 160,808 + PP_0151 0.22 +0.6 160,809 - PP_0151 0.23 +0.2 160,894 + PP_0151 0.52 +0.2 160,894 + PP_0151 0.52 -0.7 160,894 + PP_0151 0.52 +0.8 160,941 + PP_0151 0.68 -0.3 160,942 - PP_0151 0.68 +2.0 161,029 - +1.1 161,351 - PP_0152 0.59 +0.2 161,433 + PP_0152 0.81 -0.1 161,434 - PP_0152 0.81 +0.1 161,481 - -1.1 161,529 + -0.5 161,568 + -0.1 161,858 + PP_0153 0.50 +0.2 161,858 + PP_0153 0.50 -0.5 161,859 - PP_0153 0.50 -0.9 161,957 + -0.4 161,958 - +1.4 161,958 - +0.7 161,997 + -0.5 161,998 - +0.7 162,001 + -0.4 162,001 + +0.2 162,002 - -1.1
Or see this region's nucleotide sequence