Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4108 and PP_4109 are separated by 3 nucleotides PP_4109 and PP_5658 are separated by 189 nucleotides PP_5658 and PP_4111 are separated by 73 nucleotides
PP_4108: PP_4108 - 2-aminoadipate transaminase (EC 2.6.1.39) (from data) , at 4,643,134 to 4,644,384
_4108
PP_4109: PP_4109 - conserved protein of unknown function, at 4,644,388 to 4,644,690
_4109
PP_5658: PP_5658 - protein of unknown function, at 4,644,880 to 4,645,362
_5658
PP_4111: PP_4111 - Elongation factor G 2, at 4,645,436 to 4,647,547
_4111
Position (kb)
4644
4645
4646 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4643.999 kb on - strand, within PP_4108 at 4644.109 kb on + strand, within PP_4108 at 4644.110 kb on - strand, within PP_4108 at 4644.142 kb on + strand, within PP_4108 at 4644.142 kb on + strand, within PP_4108 at 4644.143 kb on - strand, within PP_4108 at 4644.389 kb on + strand at 4644.390 kb on - strand at 4644.482 kb on + strand, within PP_4109 at 4644.483 kb on - strand, within PP_4109 at 4644.483 kb on - strand, within PP_4109 at 4644.671 kb on + strand at 4644.671 kb on + strand at 4644.672 kb on - strand at 4644.672 kb on - strand at 4644.678 kb on - strand at 4644.678 kb on - strand at 4644.712 kb on + strand at 4644.712 kb on + strand at 4644.712 kb on + strand at 4644.712 kb on + strand at 4644.712 kb on + strand at 4644.712 kb on + strand at 4644.712 kb on + strand at 4644.713 kb on - strand at 4644.713 kb on - strand at 4644.713 kb on - strand at 4644.713 kb on - strand at 4644.714 kb on + strand at 4644.714 kb on + strand at 4644.715 kb on - strand at 4644.715 kb on - strand at 4644.730 kb on + strand at 4644.731 kb on - strand at 4644.731 kb on - strand at 4644.731 kb on - strand at 4644.895 kb on - strand at 4645.070 kb on + strand, within PP_5658 at 4645.071 kb on - strand, within PP_5658 at 4645.071 kb on - strand, within PP_5658 at 4645.093 kb on + strand, within PP_5658 at 4645.399 kb on - strand at 4645.427 kb on + strand at 4645.428 kb on - strand at 4645.428 kb on - strand at 4645.463 kb on + strand at 4645.464 kb on - strand at 4645.471 kb on + strand at 4645.471 kb on + strand at 4645.532 kb on + strand at 4645.532 kb on + strand at 4645.532 kb on + strand at 4645.533 kb on - strand at 4645.682 kb on + strand, within PP_4111 at 4645.682 kb on + strand, within PP_4111 at 4645.683 kb on - strand, within PP_4111 at 4645.835 kb on + strand, within PP_4111 at 4645.835 kb on + strand, within PP_4111 at 4645.835 kb on + strand, within PP_4111 at 4645.835 kb on + strand, within PP_4111 at 4645.836 kb on - strand, within PP_4111 at 4645.836 kb on - strand, within PP_4111 at 4645.836 kb on - strand, within PP_4111 at 4645.856 kb on + strand, within PP_4111 at 4645.856 kb on + strand, within PP_4111 at 4645.857 kb on - strand, within PP_4111 at 4646.012 kb on + strand, within PP_4111 at 4646.013 kb on - strand, within PP_4111 at 4646.013 kb on - strand, within PP_4111
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 4,643,999 - PP_4108 0.69 -0.5 4,644,109 + PP_4108 0.78 -0.3 4,644,110 - PP_4108 0.78 +1.0 4,644,142 + PP_4108 0.81 -0.8 4,644,142 + PP_4108 0.81 -0.1 4,644,143 - PP_4108 0.81 +0.8 4,644,389 + -0.9 4,644,390 - +0.4 4,644,482 + PP_4109 0.31 +0.2 4,644,483 - PP_4109 0.31 -0.1 4,644,483 - PP_4109 0.31 -0.6 4,644,671 + -0.8 4,644,671 + -0.7 4,644,672 - -0.4 4,644,672 - +0.2 4,644,678 - +0.2 4,644,678 - +0.3 4,644,712 + +0.6 4,644,712 + -0.5 4,644,712 + -1.4 4,644,712 + -0.7 4,644,712 + +0.2 4,644,712 + -2.3 4,644,712 + -2.8 4,644,713 - -0.7 4,644,713 - +1.6 4,644,713 - -0.7 4,644,713 - -0.4 4,644,714 + +0.1 4,644,714 + -0.3 4,644,715 - +1.0 4,644,715 - -1.9 4,644,730 + +0.5 4,644,731 - +0.8 4,644,731 - +1.0 4,644,731 - -0.1 4,644,895 - +1.0 4,645,070 + PP_5658 0.39 +1.5 4,645,071 - PP_5658 0.40 -0.3 4,645,071 - PP_5658 0.40 +0.4 4,645,093 + PP_5658 0.44 +0.6 4,645,399 - +1.7 4,645,427 + +0.1 4,645,428 - +0.6 4,645,428 - +0.0 4,645,463 + -0.7 4,645,464 - +0.3 4,645,471 + +0.2 4,645,471 + +0.7 4,645,532 + +1.3 4,645,532 + -0.1 4,645,532 + -1.0 4,645,533 - +0.6 4,645,682 + PP_4111 0.12 -0.5 4,645,682 + PP_4111 0.12 +0.4 4,645,683 - PP_4111 0.12 -2.3 4,645,835 + PP_4111 0.19 +1.1 4,645,835 + PP_4111 0.19 +1.5 4,645,835 + PP_4111 0.19 +0.1 4,645,835 + PP_4111 0.19 -2.1 4,645,836 - PP_4111 0.19 +1.0 4,645,836 - PP_4111 0.19 -1.1 4,645,836 - PP_4111 0.19 -0.6 4,645,856 + PP_4111 0.20 -0.6 4,645,856 + PP_4111 0.20 -0.6 4,645,857 - PP_4111 0.20 -1.3 4,646,012 + PP_4111 0.27 -0.8 4,646,013 - PP_4111 0.27 +0.3 4,646,013 - PP_4111 0.27 -1.3
Or see this region's nucleotide sequence