Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4718 and PP_4719 are separated by 197 nucleotides PP_4719 and PP_4720 are separated by 102 nucleotides PP_4720 and PP_4721 are separated by 12 nucleotides PP_4721 and PP_4722 overlap by 44 nucleotides PP_4722 and PP_4723 overlap by 4 nucleotides
PP_4718: PP_4718 - integral membrane ATP-dependent zinc metallopeptidase, at 5,364,642 to 5,366,555
_4718
PP_4719: PP_4719 - Ribosomal RNA large subunit methyltransferase E, at 5,366,753 to 5,367,376
_4719
PP_4720: PP_4720 - putative RNA binding protein, at 5,367,479 to 5,367,787
_4720
PP_4721: PP_4721 - conserved membrane protein of unknown function, at 5,367,800 to 5,368,270
_4721
PP_4722: PP_4722 - Transcription elongation factor GreA, at 5,368,227 to 5,368,709
_4722
PP_4723: PP_4723 - Carbamoyl-phosphate synthase large chain, at 5,368,706 to 5,371,936
_4723
Position (kb) 5367
5368 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5366.558 kb on - strand at 5366.558 kb on - strand at 5366.558 kb on - strand at 5366.558 kb on - strand at 5366.562 kb on - strand at 5366.625 kb on - strand at 5366.625 kb on - strand at 5366.625 kb on - strand at 5366.625 kb on - strand at 5366.630 kb on - strand at 5366.630 kb on - strand at 5366.630 kb on - strand at 5366.630 kb on - strand at 5366.630 kb on - strand at 5366.630 kb on - strand at 5366.679 kb on - strand at 5366.679 kb on - strand at 5366.679 kb on - strand at 5366.679 kb on - strand at 5366.722 kb on - strand at 5366.722 kb on - strand at 5366.726 kb on - strand at 5367.392 kb on + strand at 5367.392 kb on + strand at 5367.393 kb on - strand at 5367.393 kb on - strand at 5367.479 kb on - strand at 5367.510 kb on - strand, within PP_4720 at 5367.510 kb on - strand, within PP_4720 at 5367.510 kb on - strand, within PP_4720 at 5367.779 kb on + strand at 5367.779 kb on + strand at 5367.780 kb on - strand at 5367.780 kb on - strand at 5367.780 kb on - strand at 5367.791 kb on + strand at 5367.791 kb on + strand at 5367.791 kb on + strand at 5367.792 kb on - strand at 5367.792 kb on - strand at 5367.792 kb on - strand at 5367.927 kb on + strand, within PP_4721 at 5367.927 kb on + strand, within PP_4721 at 5367.927 kb on + strand, within PP_4721 at 5367.927 kb on + strand, within PP_4721 at 5367.927 kb on + strand, within PP_4721 at 5367.927 kb on + strand, within PP_4721 at 5367.928 kb on - strand, within PP_4721 at 5367.928 kb on - strand, within PP_4721 at 5367.928 kb on - strand, within PP_4721 at 5367.928 kb on - strand, within PP_4721 at 5367.928 kb on - strand, within PP_4721 at 5367.928 kb on - strand, within PP_4721 at 5367.928 kb on - strand, within PP_4721 at 5367.928 kb on - strand, within PP_4721 at 5367.931 kb on - strand, within PP_4721 at 5368.272 kb on - strand at 5368.292 kb on + strand, within PP_4722 at 5368.292 kb on + strand, within PP_4722 at 5368.292 kb on + strand, within PP_4722 at 5368.293 kb on - strand, within PP_4722 at 5368.293 kb on - strand, within PP_4722 at 5368.293 kb on - strand, within PP_4722 at 5368.293 kb on - strand, within PP_4722 at 5368.390 kb on + strand, within PP_4722 at 5368.390 kb on + strand, within PP_4722 at 5368.391 kb on - strand, within PP_4722 at 5368.391 kb on - strand, within PP_4722 at 5368.391 kb on - strand, within PP_4722 at 5368.426 kb on - strand, within PP_4722 at 5368.561 kb on + strand, within PP_4722 at 5368.561 kb on + strand, within PP_4722 at 5368.700 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 5,366,558 - +0.0 5,366,558 - +0.3 5,366,558 - -0.0 5,366,558 - -1.2 5,366,562 - +0.8 5,366,625 - +0.2 5,366,625 - +2.3 5,366,625 - +0.4 5,366,625 - -0.0 5,366,630 - +0.3 5,366,630 - +2.3 5,366,630 - -0.4 5,366,630 - +0.1 5,366,630 - +1.3 5,366,630 - +1.3 5,366,679 - -0.2 5,366,679 - -1.5 5,366,679 - +0.4 5,366,679 - +0.7 5,366,722 - -0.5 5,366,722 - +0.1 5,366,726 - +0.1 5,367,392 + +0.8 5,367,392 + +1.3 5,367,393 - +1.3 5,367,393 - +0.8 5,367,479 - -1.1 5,367,510 - PP_4720 0.10 +1.6 5,367,510 - PP_4720 0.10 +0.7 5,367,510 - PP_4720 0.10 -2.1 5,367,779 + +1.1 5,367,779 + -0.3 5,367,780 - +0.4 5,367,780 - -0.0 5,367,780 - -0.4 5,367,791 + +0.1 5,367,791 + +0.9 5,367,791 + +0.2 5,367,792 - +0.2 5,367,792 - -2.7 5,367,792 - -0.1 5,367,927 + PP_4721 0.27 +1.0 5,367,927 + PP_4721 0.27 -0.2 5,367,927 + PP_4721 0.27 -0.2 5,367,927 + PP_4721 0.27 +1.1 5,367,927 + PP_4721 0.27 +0.8 5,367,927 + PP_4721 0.27 -0.3 5,367,928 - PP_4721 0.27 -0.4 5,367,928 - PP_4721 0.27 +0.4 5,367,928 - PP_4721 0.27 +0.3 5,367,928 - PP_4721 0.27 -0.0 5,367,928 - PP_4721 0.27 -1.0 5,367,928 - PP_4721 0.27 -1.8 5,367,928 - PP_4721 0.27 +0.8 5,367,928 - PP_4721 0.27 -1.0 5,367,931 - PP_4721 0.28 -0.3 5,368,272 - -0.0 5,368,292 + PP_4722 0.13 +0.3 5,368,292 + PP_4722 0.13 -1.0 5,368,292 + PP_4722 0.13 +0.3 5,368,293 - PP_4722 0.14 -0.7 5,368,293 - PP_4722 0.14 +2.3 5,368,293 - PP_4722 0.14 +1.1 5,368,293 - PP_4722 0.14 +0.7 5,368,390 + PP_4722 0.34 +1.3 5,368,390 + PP_4722 0.34 -0.1 5,368,391 - PP_4722 0.34 -0.5 5,368,391 - PP_4722 0.34 -1.3 5,368,391 - PP_4722 0.34 +1.3 5,368,426 - PP_4722 0.41 -0.5 5,368,561 + PP_4722 0.69 -0.5 5,368,561 + PP_4722 0.69 +2.3 5,368,700 + -0.0
Or see this region's nucleotide sequence