Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3233 and PP_5591 overlap by 32 nucleotides PP_5591 and PP_3234 are separated by 63 nucleotides PP_3234 and PP_3235 are separated by 58 nucleotides PP_3235 and PP_3236 are separated by 198 nucleotides
PP_3233: PP_3233 - transcriptional regulator with 4Fe-4S cluster, Crp/Fnr family, at 3,669,707 to 3,670,438
_3233
PP_5591: PP_5591 - conserved protein of unknown function, at 3,670,407 to 3,671,132
_5591
PP_3234: PP_3234 - Heat shock protein, HSP20 family, at 3,671,196 to 3,671,891
_3234
PP_3235: PP_3235 - conserved protein of unknown function, at 3,671,950 to 3,672,342
_3235
PP_3236: PP_3236 - putative Lipoprotein OprI, at 3,672,541 to 3,672,792
_3236
Position (kb)
3671
3672 Strain fitness (log2 ratio)
-2
-1
0
1 at 3670.229 kb on + strand, within PP_3233 at 3670.295 kb on + strand, within PP_3233 at 3670.296 kb on - strand, within PP_3233 at 3670.306 kb on + strand, within PP_3233 at 3670.459 kb on + strand at 3670.459 kb on + strand at 3670.548 kb on + strand, within PP_5591 at 3670.549 kb on - strand, within PP_5591 at 3670.549 kb on - strand, within PP_5591 at 3670.609 kb on + strand, within PP_5591 at 3670.610 kb on - strand, within PP_5591 at 3670.610 kb on - strand, within PP_5591 at 3670.684 kb on - strand, within PP_5591 at 3670.841 kb on + strand, within PP_5591 at 3670.841 kb on + strand, within PP_5591 at 3670.841 kb on + strand, within PP_5591 at 3670.842 kb on - strand, within PP_5591 at 3670.842 kb on - strand, within PP_5591 at 3671.014 kb on + strand at 3671.015 kb on - strand, within PP_5591 at 3671.015 kb on - strand, within PP_5591 at 3671.173 kb on + strand at 3671.174 kb on - strand at 3671.303 kb on - strand, within PP_3234 at 3671.715 kb on + strand, within PP_3234 at 3671.715 kb on + strand, within PP_3234 at 3671.716 kb on - strand, within PP_3234 at 3671.716 kb on - strand, within PP_3234 at 3671.885 kb on + strand at 3671.886 kb on - strand at 3671.941 kb on + strand at 3671.942 kb on - strand at 3671.965 kb on + strand at 3671.966 kb on - strand at 3671.966 kb on - strand at 3671.966 kb on - strand at 3672.000 kb on + strand, within PP_3235 at 3672.001 kb on - strand, within PP_3235 at 3672.010 kb on + strand, within PP_3235 at 3672.011 kb on - strand, within PP_3235 at 3672.011 kb on - strand, within PP_3235 at 3672.038 kb on - strand, within PP_3235 at 3672.038 kb on - strand, within PP_3235 at 3672.179 kb on + strand, within PP_3235 at 3672.180 kb on - strand, within PP_3235 at 3672.240 kb on + strand, within PP_3235 at 3672.241 kb on - strand, within PP_3235 at 3672.256 kb on - strand, within PP_3235 at 3672.434 kb on + strand at 3672.506 kb on - strand at 3672.550 kb on + strand at 3672.550 kb on + strand at 3672.551 kb on - strand at 3672.551 kb on - strand at 3672.551 kb on - strand at 3672.623 kb on - strand, within PP_3236 at 3672.821 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 3,670,229 + PP_3233 0.71 +0.3 3,670,295 + PP_3233 0.80 -0.9 3,670,296 - PP_3233 0.80 +0.1 3,670,306 + PP_3233 0.82 -0.4 3,670,459 + -0.3 3,670,459 + -1.4 3,670,548 + PP_5591 0.19 -0.1 3,670,549 - PP_5591 0.20 -1.3 3,670,549 - PP_5591 0.20 -1.4 3,670,609 + PP_5591 0.28 +1.0 3,670,610 - PP_5591 0.28 +0.7 3,670,610 - PP_5591 0.28 -0.4 3,670,684 - PP_5591 0.38 -0.6 3,670,841 + PP_5591 0.60 +0.8 3,670,841 + PP_5591 0.60 -0.1 3,670,841 + PP_5591 0.60 -1.0 3,670,842 - PP_5591 0.60 +0.4 3,670,842 - PP_5591 0.60 -0.7 3,671,014 + +1.6 3,671,015 - PP_5591 0.84 -0.5 3,671,015 - PP_5591 0.84 -0.5 3,671,173 + +0.5 3,671,174 - -0.6 3,671,303 - PP_3234 0.15 +0.6 3,671,715 + PP_3234 0.75 +0.8 3,671,715 + PP_3234 0.75 +1.0 3,671,716 - PP_3234 0.75 -0.1 3,671,716 - PP_3234 0.75 +0.3 3,671,885 + +0.3 3,671,886 - -0.7 3,671,941 + +0.2 3,671,942 - +0.4 3,671,965 + +0.0 3,671,966 - +0.1 3,671,966 - -0.4 3,671,966 - -0.6 3,672,000 + PP_3235 0.13 -2.5 3,672,001 - PP_3235 0.13 +0.7 3,672,010 + PP_3235 0.15 +0.0 3,672,011 - PP_3235 0.16 +0.2 3,672,011 - PP_3235 0.16 +1.3 3,672,038 - PP_3235 0.22 +0.2 3,672,038 - PP_3235 0.22 +0.5 3,672,179 + PP_3235 0.58 -2.2 3,672,180 - PP_3235 0.59 -2.1 3,672,240 + PP_3235 0.74 -0.4 3,672,241 - PP_3235 0.74 -1.6 3,672,256 - PP_3235 0.78 -0.6 3,672,434 + +1.5 3,672,506 - +0.3 3,672,550 + +0.0 3,672,550 + +0.6 3,672,551 - +1.3 3,672,551 - +0.6 3,672,551 - +0.4 3,672,623 - PP_3236 0.33 -1.4 3,672,821 + +0.6
Or see this region's nucleotide sequence