Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1622 and PP_1623 are separated by 108 nucleotides PP_1623 and PP_1624 are separated by 474 nucleotides
PP_1622: PP_1622 - metallopeptidase, M23B subfamily, at 1,817,859 to 1,818,647
_1622
PP_1623: PP_1623 - RNA polymerase, sigma S (sigma 38) factor, at 1,818,756 to 1,819,763
_1623
PP_1624: PP_1624 - Group II intron-encoding maturase, at 1,820,238 to 1,821,659
_1624
Position (kb)
1818
1819
1820 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1818.256 kb on + strand at 1818.307 kb on + strand, within PP_1622 at 1818.308 kb on - strand, within PP_1622 at 1818.654 kb on + strand at 1818.654 kb on + strand at 1818.655 kb on - strand at 1818.676 kb on + strand at 1818.676 kb on + strand at 1818.676 kb on + strand at 1818.677 kb on - strand at 1818.751 kb on + strand at 1818.751 kb on + strand at 1818.751 kb on + strand at 1818.751 kb on + strand at 1818.752 kb on - strand at 1818.752 kb on - strand at 1818.862 kb on + strand, within PP_1623 at 1818.863 kb on - strand, within PP_1623 at 1818.863 kb on - strand, within PP_1623 at 1818.915 kb on + strand, within PP_1623 at 1818.915 kb on + strand, within PP_1623 at 1818.915 kb on + strand, within PP_1623 at 1818.916 kb on - strand, within PP_1623 at 1818.954 kb on + strand, within PP_1623 at 1818.954 kb on + strand, within PP_1623 at 1818.954 kb on + strand, within PP_1623 at 1818.955 kb on - strand, within PP_1623 at 1818.955 kb on - strand, within PP_1623 at 1818.955 kb on - strand at 1819.098 kb on + strand, within PP_1623 at 1819.206 kb on + strand, within PP_1623 at 1819.207 kb on - strand, within PP_1623 at 1819.406 kb on + strand, within PP_1623 at 1819.407 kb on - strand, within PP_1623 at 1819.407 kb on - strand, within PP_1623 at 1819.407 kb on - strand, within PP_1623 at 1819.597 kb on + strand, within PP_1623 at 1819.597 kb on + strand, within PP_1623 at 1819.598 kb on - strand at 1819.598 kb on - strand, within PP_1623 at 1819.631 kb on + strand, within PP_1623 at 1819.761 kb on + strand at 1819.761 kb on + strand at 1819.761 kb on + strand at 1819.761 kb on + strand at 1819.761 kb on + strand at 1819.766 kb on - strand at 1819.838 kb on - strand at 1820.442 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 1,818,256 + +2.0 1,818,307 + PP_1622 0.57 +0.4 1,818,308 - PP_1622 0.57 +0.4 1,818,654 + +1.0 1,818,654 + +0.4 1,818,655 - -0.2 1,818,676 + -0.8 1,818,676 + +0.5 1,818,676 + +0.0 1,818,677 - -0.0 1,818,751 + -0.1 1,818,751 + +1.2 1,818,751 + +1.7 1,818,751 + +0.2 1,818,752 - -0.2 1,818,752 - +1.5 1,818,862 + PP_1623 0.11 +0.8 1,818,863 - PP_1623 0.11 +0.7 1,818,863 - PP_1623 0.11 +1.1 1,818,915 + PP_1623 0.16 -0.6 1,818,915 + PP_1623 0.16 +1.2 1,818,915 + PP_1623 0.16 +0.1 1,818,916 - PP_1623 0.16 +0.4 1,818,954 + PP_1623 0.20 -0.6 1,818,954 + PP_1623 0.20 +0.5 1,818,954 + PP_1623 0.20 +0.3 1,818,955 - PP_1623 0.20 +0.7 1,818,955 - PP_1623 0.20 -0.7 1,818,955 - -0.0 1,819,098 + PP_1623 0.34 +0.4 1,819,206 + PP_1623 0.45 -2.9 1,819,207 - PP_1623 0.45 -2.0 1,819,406 + PP_1623 0.64 +0.7 1,819,407 - PP_1623 0.65 +0.4 1,819,407 - PP_1623 0.65 -0.8 1,819,407 - PP_1623 0.65 +0.2 1,819,597 + PP_1623 0.83 +0.9 1,819,597 + PP_1623 0.83 +0.7 1,819,598 - +2.0 1,819,598 - PP_1623 0.84 +1.0 1,819,631 + PP_1623 0.87 +0.4 1,819,761 + +0.3 1,819,761 + -1.2 1,819,761 + +0.5 1,819,761 + +0.8 1,819,761 + -0.1 1,819,766 - +0.1 1,819,838 - -1.5 1,820,442 + +2.0
Or see this region's nucleotide sequence