Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1266 and PP_1267 are separated by 72 nucleotides PP_1267 and PP_1268 overlap by 4 nucleotides PP_1268 and PP_1269 are separated by 29 nucleotides PP_1269 and PP_1270 are separated by 273 nucleotides
PP_1266: PP_1266 - putative Fusaric acid resistance protein, at 1,448,281 to 1,449,141
_1266
PP_1267: PP_1267 - toxin endoribonuclease of toxin antitoxin system SohB(PrlF)-YhaV, at 1,449,214 to 1,449,681
_1267
PP_1268: PP_1268 - putative HtrA suppressor protein sohA, at 1,449,678 to 1,450,067
_1268
PP_1269: PP_1269 - Universal stress protein family, at 1,450,097 to 1,450,543
_1269
PP_1270: PP_1270 - Transcriptional regulator, LysR family, at 1,450,817 to 1,451,722
_1270
Position (kb)
1450
1451 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1449.111 kb on + strand at 1449.112 kb on - strand at 1449.112 kb on - strand at 1449.120 kb on - strand at 1449.199 kb on + strand at 1449.200 kb on - strand at 1449.200 kb on - strand at 1449.271 kb on - strand, within PP_1267 at 1449.296 kb on + strand, within PP_1267 at 1449.296 kb on + strand, within PP_1267 at 1449.296 kb on + strand, within PP_1267 at 1449.296 kb on + strand, within PP_1267 at 1449.297 kb on - strand, within PP_1267 at 1449.297 kb on - strand, within PP_1267 at 1449.297 kb on - strand, within PP_1267 at 1449.297 kb on - strand, within PP_1267 at 1449.297 kb on - strand, within PP_1267 at 1449.297 kb on - strand, within PP_1267 at 1449.297 kb on - strand, within PP_1267 at 1449.318 kb on - strand, within PP_1267 at 1449.464 kb on + strand, within PP_1267 at 1449.493 kb on + strand, within PP_1267 at 1449.494 kb on - strand, within PP_1267 at 1449.531 kb on + strand, within PP_1267 at 1449.532 kb on - strand, within PP_1267 at 1449.542 kb on - strand, within PP_1267 at 1449.549 kb on + strand, within PP_1267 at 1449.550 kb on - strand, within PP_1267 at 1449.550 kb on - strand, within PP_1267 at 1449.552 kb on - strand, within PP_1267 at 1449.595 kb on + strand, within PP_1267 at 1449.634 kb on + strand, within PP_1267 at 1449.634 kb on + strand, within PP_1267 at 1449.635 kb on - strand at 1449.635 kb on - strand at 1449.635 kb on - strand at 1449.635 kb on - strand at 1449.652 kb on - strand at 1449.868 kb on - strand, within PP_1268 at 1450.008 kb on + strand, within PP_1268 at 1450.008 kb on + strand, within PP_1268 at 1450.008 kb on + strand, within PP_1268 at 1450.009 kb on - strand, within PP_1268 at 1450.009 kb on - strand, within PP_1268 at 1450.032 kb on - strand at 1450.032 kb on - strand at 1450.079 kb on + strand at 1450.080 kb on - strand at 1450.080 kb on - strand at 1450.366 kb on - strand, within PP_1269 at 1450.702 kb on + strand at 1450.703 kb on - strand at 1450.703 kb on - strand at 1450.712 kb on + strand at 1450.725 kb on + strand at 1450.725 kb on + strand at 1450.726 kb on - strand at 1450.726 kb on - strand at 1450.779 kb on + strand at 1450.779 kb on + strand at 1450.780 kb on - strand at 1450.996 kb on + strand, within PP_1270 at 1450.996 kb on + strand, within PP_1270 at 1450.997 kb on - strand, within PP_1270 at 1451.314 kb on + strand, within PP_1270 at 1451.315 kb on - strand, within PP_1270 at 1451.319 kb on - strand, within PP_1270 at 1451.319 kb on - strand, within PP_1270 at 1451.351 kb on - strand, within PP_1270 at 1451.351 kb on - strand, within PP_1270
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 1,449,111 + +1.1 1,449,112 - -0.9 1,449,112 - -1.1 1,449,120 - -0.3 1,449,199 + -0.4 1,449,200 - -0.5 1,449,200 - +0.6 1,449,271 - PP_1267 0.12 +0.2 1,449,296 + PP_1267 0.18 -0.6 1,449,296 + PP_1267 0.18 +0.4 1,449,296 + PP_1267 0.18 -0.4 1,449,296 + PP_1267 0.18 +1.1 1,449,297 - PP_1267 0.18 +0.2 1,449,297 - PP_1267 0.18 +0.1 1,449,297 - PP_1267 0.18 +0.2 1,449,297 - PP_1267 0.18 -0.1 1,449,297 - PP_1267 0.18 -2.3 1,449,297 - PP_1267 0.18 -0.3 1,449,297 - PP_1267 0.18 +1.9 1,449,318 - PP_1267 0.22 +0.9 1,449,464 + PP_1267 0.53 +3.1 1,449,493 + PP_1267 0.60 +0.5 1,449,494 - PP_1267 0.60 +0.3 1,449,531 + PP_1267 0.68 +0.0 1,449,532 - PP_1267 0.68 +0.2 1,449,542 - PP_1267 0.70 +0.7 1,449,549 + PP_1267 0.72 -0.2 1,449,550 - PP_1267 0.72 +0.9 1,449,550 - PP_1267 0.72 +0.1 1,449,552 - PP_1267 0.72 -0.7 1,449,595 + PP_1267 0.81 +0.1 1,449,634 + PP_1267 0.90 +2.1 1,449,634 + PP_1267 0.90 -0.7 1,449,635 - -0.9 1,449,635 - +0.3 1,449,635 - +0.8 1,449,635 - +0.6 1,449,652 - -0.9 1,449,868 - PP_1268 0.49 -1.9 1,450,008 + PP_1268 0.85 -0.5 1,450,008 + PP_1268 0.85 +0.5 1,450,008 + PP_1268 0.85 -0.4 1,450,009 - PP_1268 0.85 +0.2 1,450,009 - PP_1268 0.85 -1.1 1,450,032 - -0.5 1,450,032 - +0.1 1,450,079 + -1.3 1,450,080 - -2.3 1,450,080 - +0.5 1,450,366 - PP_1269 0.60 +0.6 1,450,702 + +0.1 1,450,703 - -0.5 1,450,703 - +0.1 1,450,712 + -0.5 1,450,725 + -0.1 1,450,725 + -1.8 1,450,726 - -1.0 1,450,726 - +1.6 1,450,779 + +0.1 1,450,779 + -0.0 1,450,780 - -0.7 1,450,996 + PP_1270 0.20 +0.7 1,450,996 + PP_1270 0.20 -0.6 1,450,997 - PP_1270 0.20 +0.4 1,451,314 + PP_1270 0.55 +0.3 1,451,315 - PP_1270 0.55 +0.3 1,451,319 - PP_1270 0.55 +0.3 1,451,319 - PP_1270 0.55 +0.1 1,451,351 - PP_1270 0.59 +0.3 1,451,351 - PP_1270 0.59 +1.1
Or see this region's nucleotide sequence