Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1240 and PP_t14 are separated by 91 nucleotides PP_t14 and PP_1241 are separated by 98 nucleotides PP_1241 and PP_1242 are separated by 356 nucleotides PP_1242 and PP_1244 are separated by 135 nucleotides
PP_1240: PP_1240 - Phosphoribosylaminoimidazole-succinocarboxamide synthase, at 1,417,542 to 1,418,252
_1240
PP_t14: PP_t14 - tRNA-Ser, at 1,418,344 to 1,418,433
_t14
PP_1241: PP_1241 - conserved protein of unknown function, at 1,418,532 to 1,418,882
_1241
PP_1242: PP_1242 - conserved protein of unknown function, at 1,419,239 to 1,419,511
_1242
PP_1244: PP_1244 - conserved protein of unknown function, at 1,419,647 to 1,420,012
_1244
Position (kb)
1418
1419 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1418.251 kb on - strand at 1418.434 kb on + strand at 1418.434 kb on + strand at 1418.435 kb on - strand at 1418.513 kb on - strand at 1418.529 kb on - strand at 1418.574 kb on + strand, within PP_1241 at 1418.575 kb on - strand, within PP_1241 at 1418.575 kb on - strand, within PP_1241 at 1418.580 kb on + strand, within PP_1241 at 1418.580 kb on + strand, within PP_1241 at 1418.580 kb on + strand, within PP_1241 at 1418.580 kb on + strand, within PP_1241 at 1418.643 kb on - strand, within PP_1241 at 1418.744 kb on + strand, within PP_1241 at 1418.744 kb on + strand, within PP_1241 at 1418.745 kb on - strand, within PP_1241 at 1418.894 kb on + strand at 1418.894 kb on + strand at 1418.894 kb on + strand at 1418.955 kb on + strand at 1418.956 kb on - strand at 1418.977 kb on - strand at 1418.989 kb on + strand at 1418.989 kb on + strand at 1418.989 kb on + strand at 1418.989 kb on + strand at 1418.989 kb on + strand at 1418.990 kb on - strand at 1418.990 kb on - strand at 1418.990 kb on - strand at 1418.991 kb on + strand at 1418.991 kb on + strand at 1418.991 kb on + strand at 1418.991 kb on + strand at 1418.992 kb on - strand at 1419.118 kb on - strand at 1419.118 kb on - strand at 1419.127 kb on + strand at 1419.167 kb on + strand at 1419.211 kb on - strand at 1419.300 kb on + strand, within PP_1242 at 1419.301 kb on - strand, within PP_1242 at 1419.301 kb on - strand, within PP_1242 at 1419.366 kb on + strand, within PP_1242 at 1419.366 kb on + strand, within PP_1242 at 1419.366 kb on + strand, within PP_1242 at 1419.459 kb on + strand, within PP_1242 at 1419.459 kb on + strand, within PP_1242 at 1419.460 kb on - strand, within PP_1242 at 1419.460 kb on - strand, within PP_1242 at 1419.578 kb on + strand at 1419.579 kb on - strand at 1419.579 kb on - strand at 1419.649 kb on - strand at 1419.674 kb on + strand at 1419.674 kb on + strand at 1419.674 kb on + strand at 1419.675 kb on - strand at 1419.726 kb on - strand, within PP_1244 at 1419.824 kb on + strand, within PP_1244 at 1419.824 kb on + strand, within PP_1244 at 1419.824 kb on + strand, within PP_1244 at 1419.824 kb on + strand, within PP_1244 at 1419.825 kb on - strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 1,418,251 - -0.2 1,418,434 + -1.2 1,418,434 + -0.6 1,418,435 - -0.5 1,418,513 - +1.1 1,418,529 - +0.6 1,418,574 + PP_1241 0.12 +0.5 1,418,575 - PP_1241 0.12 +0.5 1,418,575 - PP_1241 0.12 -0.1 1,418,580 + PP_1241 0.14 +1.3 1,418,580 + PP_1241 0.14 +1.6 1,418,580 + PP_1241 0.14 +0.7 1,418,580 + PP_1241 0.14 +0.1 1,418,643 - PP_1241 0.32 -0.5 1,418,744 + PP_1241 0.60 -0.3 1,418,744 + PP_1241 0.60 +0.8 1,418,745 - PP_1241 0.61 -0.1 1,418,894 + -0.3 1,418,894 + +1.1 1,418,894 + +0.0 1,418,955 + -0.1 1,418,956 - +0.6 1,418,977 - -0.1 1,418,989 + -0.2 1,418,989 + -0.8 1,418,989 + +1.4 1,418,989 + -0.4 1,418,989 + +1.1 1,418,990 - +1.4 1,418,990 - -0.6 1,418,990 - -1.7 1,418,991 + -1.8 1,418,991 + +0.1 1,418,991 + +1.1 1,418,991 + +0.3 1,418,992 - +0.9 1,419,118 - +1.3 1,419,118 - +0.6 1,419,127 + +2.1 1,419,167 + +0.3 1,419,211 - +1.4 1,419,300 + PP_1242 0.22 -0.8 1,419,301 - PP_1242 0.23 +0.5 1,419,301 - PP_1242 0.23 +0.5 1,419,366 + PP_1242 0.47 +0.4 1,419,366 + PP_1242 0.47 +0.9 1,419,366 + PP_1242 0.47 +1.3 1,419,459 + PP_1242 0.81 -0.1 1,419,459 + PP_1242 0.81 -0.4 1,419,460 - PP_1242 0.81 -1.4 1,419,460 - PP_1242 0.81 -0.2 1,419,578 + -0.2 1,419,579 - -0.5 1,419,579 - +0.5 1,419,649 - +0.2 1,419,674 + -0.7 1,419,674 + -0.2 1,419,674 + -0.0 1,419,675 - -0.6 1,419,726 - PP_1244 0.22 +0.1 1,419,824 + PP_1244 0.48 +0.8 1,419,824 + PP_1244 0.48 +0.0 1,419,824 + PP_1244 0.48 -1.0 1,419,824 + PP_1244 0.48 +0.5 1,419,825 - PP_1244 0.49 -0.1 1,419,828 + PP_1244 0.49 -0.9 1,419,828 + PP_1244 0.49 -1.0 1,419,828 + PP_1244 0.49 -0.6 1,419,828 + PP_1244 0.49 +1.6 1,419,828 + PP_1244 0.49 +0.0
Or see this region's nucleotide sequence