Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0681 and PP_0682 are separated by 189 nucleotides PP_0682 and PP_0683 are separated by 76 nucleotides PP_0683 and PP_0684 are separated by 85 nucleotides PP_0684 and PP_0685 are separated by 26 nucleotides
PP_0681: PP_0681 - conserved protein of unknown function, at 796,460 to 796,798
_0681
PP_0682: PP_0682 - conserved inner membrane protein of unknown function, UPF0114 family, at 796,988 to 797,476
_0682
PP_0683: PP_0683 - conserved protein of unknown function, at 797,553 to 797,942
_0683
PP_0684: PP_0684 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 798,028 to 798,645
_0684
PP_0685: PP_0685 - conserved protein of unknown function, at 798,672 to 798,917
_0685
Position (kb)
797
798 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 796.683 kb on - strand, within PP_0681 at 796.776 kb on - strand at 796.852 kb on + strand at 796.852 kb on + strand at 796.853 kb on - strand at 796.927 kb on + strand at 796.928 kb on - strand at 796.944 kb on + strand at 796.944 kb on + strand at 796.945 kb on - strand at 796.945 kb on - strand at 796.945 kb on - strand at 796.962 kb on + strand at 796.963 kb on - strand at 796.997 kb on - strand at 797.015 kb on + strand at 797.015 kb on + strand at 797.015 kb on + strand at 797.016 kb on - strand at 797.016 kb on - strand at 797.016 kb on - strand at 797.045 kb on + strand, within PP_0682 at 797.045 kb on + strand, within PP_0682 at 797.046 kb on - strand, within PP_0682 at 797.390 kb on + strand, within PP_0682 at 797.402 kb on + strand, within PP_0682 at 797.402 kb on + strand, within PP_0682 at 797.402 kb on + strand, within PP_0682 at 797.403 kb on - strand, within PP_0682 at 797.403 kb on - strand, within PP_0682 at 797.533 kb on + strand at 797.591 kb on - strand at 797.718 kb on + strand, within PP_0683 at 797.719 kb on - strand, within PP_0683 at 797.719 kb on - strand, within PP_0683 at 797.719 kb on - strand, within PP_0683 at 797.940 kb on + strand at 797.940 kb on + strand at 797.967 kb on - strand at 798.070 kb on + strand at 798.070 kb on + strand at 798.070 kb on + strand at 798.399 kb on + strand, within PP_0684 at 798.400 kb on - strand, within PP_0684 at 798.407 kb on - strand at 798.448 kb on + strand at 798.448 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.547 kb on + strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.548 kb on - strand, within PP_0684 at 798.569 kb on - strand, within PP_0684 at 798.643 kb on + strand at 798.644 kb on - strand at 798.644 kb on - strand at 798.713 kb on - strand, within PP_0685 at 798.713 kb on - strand, within PP_0685 at 798.874 kb on + strand, within PP_0685 at 798.910 kb on + strand at 798.910 kb on + strand at 798.911 kb on - strand at 798.911 kb on - strand at 798.921 kb on + strand at 798.921 kb on + strand at 798.922 kb on - strand at 798.922 kb on - strand at 798.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 796,683 - PP_0681 0.66 +0.3 796,776 - -0.7 796,852 + +0.3 796,852 + -0.8 796,853 - -0.2 796,927 + -0.9 796,928 - +1.1 796,944 + +2.3 796,944 + +0.3 796,945 - +1.9 796,945 - +0.2 796,945 - -0.1 796,962 + -1.0 796,963 - +0.0 796,997 - -0.3 797,015 + +0.5 797,015 + +1.3 797,015 + -1.0 797,016 - -0.4 797,016 - +0.5 797,016 - -0.5 797,045 + PP_0682 0.12 -0.3 797,045 + PP_0682 0.12 +0.7 797,046 - PP_0682 0.12 -0.7 797,390 + PP_0682 0.82 -0.3 797,402 + PP_0682 0.85 +1.9 797,402 + PP_0682 0.85 +0.5 797,402 + PP_0682 0.85 -0.3 797,403 - PP_0682 0.85 +0.1 797,403 - PP_0682 0.85 -0.2 797,533 + -0.2 797,591 - -0.6 797,718 + PP_0683 0.42 -1.1 797,719 - PP_0683 0.43 -1.0 797,719 - PP_0683 0.43 +0.7 797,719 - PP_0683 0.43 -3.1 797,940 + -0.1 797,940 + +0.2 797,967 - -1.1 798,070 + -2.4 798,070 + +0.5 798,070 + -0.4 798,399 + PP_0684 0.60 -0.4 798,400 - PP_0684 0.60 -0.4 798,407 - -0.6 798,448 + -0.0 798,448 + PP_0684 0.68 -1.3 798,547 + PP_0684 0.84 +0.5 798,547 + PP_0684 0.84 +0.5 798,547 + PP_0684 0.84 -0.6 798,547 + PP_0684 0.84 -0.6 798,547 + PP_0684 0.84 -2.8 798,548 - PP_0684 0.84 -0.2 798,548 - PP_0684 0.84 -0.5 798,548 - PP_0684 0.84 +0.2 798,548 - PP_0684 0.84 -1.3 798,548 - PP_0684 0.84 +1.0 798,548 - PP_0684 0.84 -2.7 798,569 - PP_0684 0.88 -0.1 798,643 + -1.0 798,644 - -2.3 798,644 - +0.7 798,713 - PP_0685 0.17 +0.2 798,713 - PP_0685 0.17 +0.5 798,874 + PP_0685 0.82 +0.7 798,910 + -0.0 798,910 + +2.3 798,911 - -0.3 798,911 - +1.4 798,921 + -0.3 798,921 + -0.0 798,922 - -1.8 798,922 - +0.7 798,922 - +0.1
Or see this region's nucleotide sequence