Experiment: MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0619 and PP_0620 are separated by 90 nucleotides PP_0620 and PP_0621 are separated by 266 nucleotides PP_0621 and PP_0622 are separated by 13 nucleotides
PP_0619: PP_0619 - Branched-chain amino acid ABC transporter, periplasmic amino acid-binding protein, at 724,696 to 725,898
_0619
PP_0620: PP_0620 - GntR family transcriptional regulator, at 725,989 to 726,753
_0620
PP_0621: PP_0621 - conserved protein of unknown function, at 727,020 to 727,331
_0621
PP_0622: PP_0622 - Outer membrane protein assembly factor BamD, at 727,345 to 728,364
_0622
Position (kb) 725
726
727 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 725.000 kb on + strand, within PP_0619 at 725.000 kb on + strand, within PP_0619 at 725.000 kb on + strand, within PP_0619 at 725.000 kb on + strand, within PP_0619 at 725.060 kb on + strand, within PP_0619 at 725.075 kb on + strand, within PP_0619 at 725.075 kb on + strand, within PP_0619 at 725.294 kb on + strand, within PP_0619 at 725.295 kb on - strand, within PP_0619 at 725.368 kb on + strand, within PP_0619 at 725.368 kb on + strand, within PP_0619 at 725.368 kb on + strand, within PP_0619 at 725.368 kb on + strand, within PP_0619 at 725.368 kb on + strand, within PP_0619 at 725.368 kb on + strand, within PP_0619 at 725.369 kb on - strand, within PP_0619 at 725.369 kb on - strand, within PP_0619 at 725.372 kb on + strand, within PP_0619 at 725.372 kb on + strand, within PP_0619 at 725.373 kb on - strand, within PP_0619 at 725.390 kb on + strand, within PP_0619 at 725.391 kb on - strand, within PP_0619 at 725.391 kb on - strand, within PP_0619 at 725.453 kb on + strand, within PP_0619 at 725.453 kb on + strand, within PP_0619 at 725.453 kb on + strand, within PP_0619 at 725.453 kb on + strand, within PP_0619 at 725.453 kb on + strand, within PP_0619 at 725.453 kb on + strand, within PP_0619 at 725.453 kb on + strand, within PP_0619 at 725.454 kb on - strand, within PP_0619 at 725.454 kb on - strand, within PP_0619 at 725.454 kb on - strand, within PP_0619 at 725.454 kb on - strand, within PP_0619 at 725.454 kb on - strand, within PP_0619 at 725.454 kb on - strand, within PP_0619 at 725.454 kb on - strand, within PP_0619 at 725.750 kb on + strand, within PP_0619 at 725.759 kb on + strand, within PP_0619 at 725.759 kb on + strand, within PP_0619 at 725.795 kb on + strand at 725.796 kb on - strand at 725.796 kb on - strand at 725.796 kb on - strand at 725.852 kb on - strand at 725.852 kb on - strand at 725.852 kb on - strand at 725.852 kb on - strand at 725.852 kb on - strand at 725.886 kb on + strand at 725.886 kb on + strand at 725.887 kb on - strand at 725.902 kb on + strand at 725.902 kb on + strand at 725.903 kb on - strand at 725.903 kb on - strand at 725.921 kb on - strand at 725.923 kb on + strand at 725.923 kb on + strand at 725.923 kb on + strand at 725.924 kb on - strand at 725.924 kb on - strand at 725.924 kb on - strand at 725.924 kb on - strand at 725.924 kb on - strand at 725.980 kb on - strand at 726.095 kb on - strand, within PP_0620 at 726.185 kb on + strand, within PP_0620 at 726.186 kb on - strand, within PP_0620 at 726.377 kb on + strand, within PP_0620 at 726.446 kb on + strand, within PP_0620 at 726.446 kb on + strand, within PP_0620 at 726.447 kb on - strand, within PP_0620 at 726.447 kb on - strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.483 kb on - strand, within PP_0620 at 726.839 kb on + strand at 726.840 kb on - strand at 726.840 kb on - strand at 726.840 kb on - strand at 726.867 kb on + strand at 727.066 kb on + strand, within PP_0621 at 727.066 kb on + strand, within PP_0621 at 727.066 kb on + strand, within PP_0621 at 727.067 kb on - strand, within PP_0621 at 727.067 kb on - strand, within PP_0621 at 727.067 kb on - strand, within PP_0621 at 727.189 kb on + strand, within PP_0621 at 727.189 kb on + strand, within PP_0621 at 727.190 kb on - strand, within PP_0621 at 727.370 kb on + strand at 727.371 kb on - strand at 727.371 kb on - strand at 727.371 kb on - strand at 727.371 kb on - strand at 727.444 kb on - strand at 727.643 kb on + strand, within PP_0622
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL remove 725,000 + PP_0619 0.25 +0.5 725,000 + PP_0619 0.25 +0.7 725,000 + PP_0619 0.25 +0.2 725,000 + PP_0619 0.25 -1.8 725,060 + PP_0619 0.30 -1.2 725,075 + PP_0619 0.32 -2.6 725,075 + PP_0619 0.32 +1.2 725,294 + PP_0619 0.50 +0.5 725,295 - PP_0619 0.50 +2.3 725,368 + PP_0619 0.56 -0.9 725,368 + PP_0619 0.56 -1.6 725,368 + PP_0619 0.56 +0.5 725,368 + PP_0619 0.56 +1.0 725,368 + PP_0619 0.56 -2.2 725,368 + PP_0619 0.56 +1.3 725,369 - PP_0619 0.56 +0.0 725,369 - PP_0619 0.56 +1.9 725,372 + PP_0619 0.56 -0.6 725,372 + PP_0619 0.56 -0.0 725,373 - PP_0619 0.56 +1.3 725,390 + PP_0619 0.58 +0.2 725,391 - PP_0619 0.58 -0.6 725,391 - PP_0619 0.58 -2.2 725,453 + PP_0619 0.63 -0.3 725,453 + PP_0619 0.63 -0.0 725,453 + PP_0619 0.63 +0.8 725,453 + PP_0619 0.63 -1.1 725,453 + PP_0619 0.63 +1.1 725,453 + PP_0619 0.63 +0.2 725,453 + PP_0619 0.63 +1.1 725,454 - PP_0619 0.63 -0.2 725,454 - PP_0619 0.63 +0.2 725,454 - PP_0619 0.63 +0.7 725,454 - PP_0619 0.63 +0.3 725,454 - PP_0619 0.63 +1.3 725,454 - PP_0619 0.63 +0.3 725,454 - PP_0619 0.63 +0.5 725,750 + PP_0619 0.88 +0.6 725,759 + PP_0619 0.88 -1.3 725,759 + PP_0619 0.88 -0.0 725,795 + -1.9 725,796 - -0.0 725,796 - +0.1 725,796 - -2.8 725,852 - +0.3 725,852 - +0.4 725,852 - -0.7 725,852 - +0.5 725,852 - +0.6 725,886 + +0.4 725,886 + -0.7 725,887 - -0.1 725,902 + +0.7 725,902 + +0.3 725,903 - +0.8 725,903 - +0.9 725,921 - +0.3 725,923 + -1.2 725,923 + +0.5 725,923 + -0.5 725,924 - -0.9 725,924 - -0.7 725,924 - +0.0 725,924 - -0.2 725,924 - -0.1 725,980 - +1.2 726,095 - PP_0620 0.14 -0.5 726,185 + PP_0620 0.26 -1.0 726,186 - PP_0620 0.26 -0.9 726,377 + PP_0620 0.51 +0.3 726,446 + PP_0620 0.60 -0.5 726,446 + PP_0620 0.60 -1.4 726,447 - PP_0620 0.60 -0.1 726,447 - PP_0620 0.60 +0.1 726,482 + PP_0620 0.64 -0.3 726,482 + PP_0620 0.64 +0.3 726,482 + PP_0620 0.64 +0.8 726,482 + PP_0620 0.64 -1.1 726,483 - PP_0620 0.65 -1.5 726,839 + -2.1 726,840 - -0.2 726,840 - -1.9 726,840 - +0.6 726,867 + +1.0 727,066 + PP_0621 0.15 -2.1 727,066 + PP_0621 0.15 +1.3 727,066 + PP_0621 0.15 +1.2 727,067 - PP_0621 0.15 +1.6 727,067 - PP_0621 0.15 +0.0 727,067 - PP_0621 0.15 -1.0 727,189 + PP_0621 0.54 -0.7 727,189 + PP_0621 0.54 -0.5 727,190 - PP_0621 0.54 +0.4 727,370 + -0.6 727,371 - +1.9 727,371 - +1.3 727,371 - -1.0 727,371 - -0.1 727,444 - -0.5 727,643 + PP_0622 0.29 +0.0
Or see this region's nucleotide sequence