Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4492 and PP_4493 are separated by 113 nucleotides PP_4493 and PP_4494 are separated by 152 nucleotides
PP_4492: PP_4492 - carbohydrate efflux transporter, at 5,103,775 to 5,104,977
_4492
PP_4493: PP_4493 - D-2-hydroxyglutarate dehydrogenase (EC 1.1.99.39) (from data) , at 5,105,091 to 5,108,111
_4493
PP_4494: PP_4494 - putative LysR-type transcriptional activator protein, at 5,108,264 to 5,109,148
_4494
Position (kb)
5105
5106
5107
5108
5109 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5104.155 kb on - strand, within PP_4492 at 5104.155 kb on - strand, within PP_4492 at 5104.240 kb on + strand, within PP_4492 at 5104.241 kb on - strand, within PP_4492 at 5104.388 kb on + strand, within PP_4492 at 5104.389 kb on - strand, within PP_4492 at 5104.389 kb on - strand, within PP_4492 at 5104.389 kb on - strand, within PP_4492 at 5104.421 kb on + strand, within PP_4492 at 5104.421 kb on + strand, within PP_4492 at 5104.422 kb on - strand, within PP_4492 at 5104.422 kb on - strand, within PP_4492 at 5104.492 kb on + strand, within PP_4492 at 5104.492 kb on + strand, within PP_4492 at 5104.492 kb on + strand, within PP_4492 at 5104.495 kb on + strand, within PP_4492 at 5104.495 kb on + strand, within PP_4492 at 5104.495 kb on + strand, within PP_4492 at 5104.495 kb on + strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.496 kb on - strand, within PP_4492 at 5104.795 kb on + strand, within PP_4492 at 5104.796 kb on - strand, within PP_4492 at 5104.927 kb on + strand at 5104.927 kb on + strand at 5104.928 kb on - strand at 5104.928 kb on - strand at 5104.962 kb on + strand at 5104.962 kb on + strand at 5104.963 kb on - strand at 5104.963 kb on - strand at 5104.963 kb on - strand at 5104.963 kb on - strand at 5105.037 kb on - strand at 5105.104 kb on + strand at 5105.105 kb on - strand at 5105.105 kb on - strand at 5105.165 kb on + strand at 5105.235 kb on + strand at 5105.276 kb on - strand at 5105.276 kb on - strand at 5105.276 kb on - strand at 5105.276 kb on - strand at 5105.288 kb on + strand at 5105.289 kb on - strand at 5105.289 kb on - strand at 5105.289 kb on - strand at 5105.289 kb on - strand at 5105.637 kb on + strand, within PP_4493 at 5105.637 kb on + strand, within PP_4493 at 5105.637 kb on + strand, within PP_4493 at 5105.637 kb on + strand, within PP_4493 at 5105.947 kb on + strand, within PP_4493 at 5105.948 kb on - strand, within PP_4493 at 5105.948 kb on - strand, within PP_4493 at 5105.971 kb on + strand, within PP_4493 at 5105.972 kb on - strand, within PP_4493 at 5106.026 kb on - strand, within PP_4493 at 5106.037 kb on + strand at 5106.129 kb on - strand, within PP_4493 at 5106.136 kb on + strand, within PP_4493 at 5106.367 kb on + strand, within PP_4493 at 5106.367 kb on + strand, within PP_4493 at 5106.368 kb on - strand, within PP_4493 at 5106.368 kb on - strand, within PP_4493 at 5106.368 kb on - strand, within PP_4493 at 5106.368 kb on - strand, within PP_4493 at 5106.368 kb on - strand, within PP_4493 at 5106.368 kb on - strand, within PP_4493 at 5106.368 kb on - strand, within PP_4493 at 5106.373 kb on + strand, within PP_4493 at 5106.373 kb on + strand, within PP_4493 at 5106.373 kb on + strand, within PP_4493 at 5106.374 kb on - strand, within PP_4493 at 5106.440 kb on + strand, within PP_4493 at 5106.577 kb on + strand, within PP_4493 at 5106.592 kb on + strand, within PP_4493 at 5106.604 kb on + strand, within PP_4493 at 5106.604 kb on + strand, within PP_4493 at 5106.605 kb on - strand, within PP_4493 at 5106.779 kb on - strand, within PP_4493 at 5106.823 kb on + strand, within PP_4493 at 5106.823 kb on + strand, within PP_4493 at 5106.824 kb on - strand, within PP_4493 at 5106.824 kb on - strand, within PP_4493 at 5106.847 kb on - strand, within PP_4493 at 5106.920 kb on + strand, within PP_4493 at 5106.920 kb on + strand, within PP_4493 at 5106.921 kb on - strand, within PP_4493 at 5106.921 kb on - strand, within PP_4493 at 5106.929 kb on + strand, within PP_4493 at 5106.929 kb on + strand, within PP_4493 at 5106.929 kb on + strand, within PP_4493 at 5106.930 kb on - strand, within PP_4493 at 5106.930 kb on - strand, within PP_4493 at 5106.930 kb on - strand, within PP_4493 at 5106.930 kb on - strand, within PP_4493 at 5106.932 kb on - strand, within PP_4493 at 5107.243 kb on + strand, within PP_4493 at 5107.267 kb on + strand, within PP_4493 at 5107.267 kb on + strand, within PP_4493 at 5107.267 kb on + strand, within PP_4493 at 5107.267 kb on + strand, within PP_4493 at 5107.310 kb on - strand, within PP_4493 at 5107.338 kb on + strand, within PP_4493 at 5107.339 kb on - strand, within PP_4493 at 5107.339 kb on - strand, within PP_4493 at 5107.475 kb on - strand, within PP_4493 at 5107.606 kb on + strand, within PP_4493 at 5107.607 kb on - strand, within PP_4493 at 5107.607 kb on - strand, within PP_4493 at 5107.821 kb on - strand at 5107.972 kb on + strand at 5107.996 kb on - strand at 5108.012 kb on - strand at 5108.131 kb on - strand at 5108.137 kb on + strand at 5108.150 kb on - strand at 5108.191 kb on + strand at 5108.192 kb on - strand at 5108.192 kb on - strand at 5108.194 kb on - strand at 5108.260 kb on - strand at 5108.337 kb on - strand at 5108.337 kb on - strand at 5108.337 kb on - strand at 5108.391 kb on + strand, within PP_4494 at 5108.391 kb on + strand, within PP_4494 at 5108.392 kb on - strand, within PP_4494 at 5108.392 kb on - strand, within PP_4494 at 5108.448 kb on + strand, within PP_4494 at 5108.449 kb on - strand, within PP_4494 at 5108.449 kb on - strand, within PP_4494 at 5108.475 kb on - strand, within PP_4494 at 5108.537 kb on + strand, within PP_4494 at 5108.538 kb on - strand, within PP_4494 at 5108.550 kb on + strand, within PP_4494 at 5108.591 kb on - strand, within PP_4494 at 5108.591 kb on - strand, within PP_4494 at 5108.621 kb on + strand, within PP_4494 at 5108.621 kb on + strand, within PP_4494 at 5108.962 kb on - strand, within PP_4494 at 5108.997 kb on + strand, within PP_4494 at 5108.998 kb on - strand, within PP_4494 at 5108.998 kb on - strand, within PP_4494 at 5109.032 kb on + strand, within PP_4494 at 5109.032 kb on + strand, within PP_4494 at 5109.032 kb on + strand, within PP_4494 at 5109.032 kb on + strand, within PP_4494 at 5109.032 kb on + strand, within PP_4494 at 5109.032 kb on + strand, within PP_4494 at 5109.032 kb on + strand, within PP_4494 at 5109.032 kb on + strand, within PP_4494 at 5109.033 kb on - strand, within PP_4494 at 5109.033 kb on - strand, within PP_4494 at 5109.033 kb on - strand, within PP_4494 at 5109.068 kb on + strand at 5109.068 kb on + strand at 5109.068 kb on + strand at 5109.071 kb on + strand at 5109.071 kb on + strand at 5109.072 kb on - strand at 5109.080 kb on + strand at 5109.081 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 5,104,155 - PP_4492 0.32 -0.5 5,104,155 - PP_4492 0.32 -1.5 5,104,240 + PP_4492 0.39 -0.1 5,104,241 - PP_4492 0.39 -3.9 5,104,388 + PP_4492 0.51 +2.3 5,104,389 - PP_4492 0.51 +2.3 5,104,389 - PP_4492 0.51 +0.4 5,104,389 - PP_4492 0.51 +0.3 5,104,421 + PP_4492 0.54 -0.2 5,104,421 + PP_4492 0.54 +1.3 5,104,422 - PP_4492 0.54 -0.1 5,104,422 - PP_4492 0.54 -0.9 5,104,492 + PP_4492 0.60 -1.2 5,104,492 + PP_4492 0.60 +0.7 5,104,492 + PP_4492 0.60 +0.1 5,104,495 + PP_4492 0.60 +0.8 5,104,495 + PP_4492 0.60 +1.0 5,104,495 + PP_4492 0.60 -0.7 5,104,495 + PP_4492 0.60 +1.2 5,104,496 - PP_4492 0.60 +0.2 5,104,496 - PP_4492 0.60 -0.6 5,104,496 - PP_4492 0.60 +0.6 5,104,496 - PP_4492 0.60 -0.7 5,104,496 - PP_4492 0.60 -0.5 5,104,496 - PP_4492 0.60 -0.5 5,104,496 - PP_4492 0.60 -1.1 5,104,496 - PP_4492 0.60 -0.1 5,104,496 - PP_4492 0.60 -0.2 5,104,496 - PP_4492 0.60 -0.3 5,104,795 + PP_4492 0.85 +1.7 5,104,796 - PP_4492 0.85 +0.4 5,104,927 + +0.7 5,104,927 + -0.6 5,104,928 - +0.1 5,104,928 - -1.3 5,104,962 + -0.5 5,104,962 + -1.0 5,104,963 - +0.8 5,104,963 - +2.7 5,104,963 - -1.1 5,104,963 - +0.1 5,105,037 - +0.7 5,105,104 + +0.1 5,105,105 - +0.7 5,105,105 - +0.3 5,105,165 + +0.6 5,105,235 + -1.8 5,105,276 - +0.4 5,105,276 - +1.3 5,105,276 - -0.8 5,105,276 - +1.9 5,105,288 + +0.4 5,105,289 - -2.5 5,105,289 - -1.6 5,105,289 - -0.1 5,105,289 - +0.3 5,105,637 + PP_4493 0.18 +0.3 5,105,637 + PP_4493 0.18 +0.2 5,105,637 + PP_4493 0.18 -0.3 5,105,637 + PP_4493 0.18 +0.4 5,105,947 + PP_4493 0.28 -0.7 5,105,948 - PP_4493 0.28 +0.3 5,105,948 - PP_4493 0.28 -0.7 5,105,971 + PP_4493 0.29 -0.1 5,105,972 - PP_4493 0.29 -0.4 5,106,026 - PP_4493 0.31 -0.7 5,106,037 + +1.3 5,106,129 - PP_4493 0.34 -0.3 5,106,136 + PP_4493 0.35 -0.1 5,106,367 + PP_4493 0.42 -1.9 5,106,367 + PP_4493 0.42 -1.1 5,106,368 - PP_4493 0.42 -0.8 5,106,368 - PP_4493 0.42 -2.4 5,106,368 - PP_4493 0.42 -0.6 5,106,368 - PP_4493 0.42 +0.3 5,106,368 - PP_4493 0.42 +0.3 5,106,368 - PP_4493 0.42 -0.5 5,106,368 - PP_4493 0.42 -0.3 5,106,373 + PP_4493 0.42 -0.8 5,106,373 + PP_4493 0.42 -0.9 5,106,373 + PP_4493 0.42 -1.3 5,106,374 - PP_4493 0.42 -0.1 5,106,440 + PP_4493 0.45 -1.9 5,106,577 + PP_4493 0.49 -0.7 5,106,592 + PP_4493 0.50 +0.1 5,106,604 + PP_4493 0.50 -0.9 5,106,604 + PP_4493 0.50 -0.7 5,106,605 - PP_4493 0.50 +0.0 5,106,779 - PP_4493 0.56 +0.2 5,106,823 + PP_4493 0.57 +1.7 5,106,823 + PP_4493 0.57 -0.3 5,106,824 - PP_4493 0.57 -1.1 5,106,824 - PP_4493 0.57 -0.3 5,106,847 - PP_4493 0.58 -0.5 5,106,920 + PP_4493 0.61 -0.3 5,106,920 + PP_4493 0.61 -0.8 5,106,921 - PP_4493 0.61 -0.2 5,106,921 - PP_4493 0.61 -0.1 5,106,929 + PP_4493 0.61 -0.5 5,106,929 + PP_4493 0.61 -1.8 5,106,929 + PP_4493 0.61 +0.5 5,106,930 - PP_4493 0.61 -1.2 5,106,930 - PP_4493 0.61 +0.9 5,106,930 - PP_4493 0.61 -0.1 5,106,930 - PP_4493 0.61 -1.6 5,106,932 - PP_4493 0.61 +0.8 5,107,243 + PP_4493 0.71 -0.1 5,107,267 + PP_4493 0.72 -1.1 5,107,267 + PP_4493 0.72 -1.4 5,107,267 + PP_4493 0.72 -0.1 5,107,267 + PP_4493 0.72 -0.3 5,107,310 - PP_4493 0.73 -0.5 5,107,338 + PP_4493 0.74 -0.1 5,107,339 - PP_4493 0.74 +0.3 5,107,339 - PP_4493 0.74 -2.0 5,107,475 - PP_4493 0.79 -1.0 5,107,606 + PP_4493 0.83 -0.4 5,107,607 - PP_4493 0.83 -0.8 5,107,607 - PP_4493 0.83 -1.1 5,107,821 - +1.7 5,107,972 + +0.8 5,107,996 - +0.7 5,108,012 - +0.2 5,108,131 - +0.5 5,108,137 + -1.7 5,108,150 - +0.6 5,108,191 + +0.2 5,108,192 - +0.7 5,108,192 - +0.9 5,108,194 - +0.8 5,108,260 - +0.9 5,108,337 - +1.3 5,108,337 - +0.1 5,108,337 - -1.6 5,108,391 + PP_4494 0.14 +0.7 5,108,391 + PP_4494 0.14 -0.1 5,108,392 - PP_4494 0.14 -2.3 5,108,392 - PP_4494 0.14 +0.2 5,108,448 + PP_4494 0.21 +0.8 5,108,449 - PP_4494 0.21 +0.4 5,108,449 - PP_4494 0.21 +0.5 5,108,475 - PP_4494 0.24 -0.8 5,108,537 + PP_4494 0.31 +0.3 5,108,538 - PP_4494 0.31 +0.0 5,108,550 + PP_4494 0.32 -0.2 5,108,591 - PP_4494 0.37 +0.1 5,108,591 - PP_4494 0.37 +0.4 5,108,621 + PP_4494 0.40 -0.8 5,108,621 + PP_4494 0.40 +1.3 5,108,962 - PP_4494 0.79 -0.6 5,108,997 + PP_4494 0.83 +0.2 5,108,998 - PP_4494 0.83 +0.3 5,108,998 - PP_4494 0.83 -0.5 5,109,032 + PP_4494 0.87 +2.6 5,109,032 + PP_4494 0.87 -0.2 5,109,032 + PP_4494 0.87 +0.5 5,109,032 + PP_4494 0.87 -1.3 5,109,032 + PP_4494 0.87 -0.4 5,109,032 + PP_4494 0.87 +0.7 5,109,032 + PP_4494 0.87 +0.2 5,109,032 + PP_4494 0.87 -1.1 5,109,033 - PP_4494 0.87 -0.1 5,109,033 - PP_4494 0.87 -1.6 5,109,033 - PP_4494 0.87 -0.1 5,109,068 + +1.1 5,109,068 + +0.2 5,109,068 + -0.1 5,109,071 + -0.1 5,109,071 + +0.4 5,109,072 - -1.3 5,109,080 + +0.6 5,109,081 - +0.7
Or see this region's nucleotide sequence