Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3419 and PP_3420 overlap by 32 nucleotides PP_3420 and PP_3421 are separated by 307 nucleotides
PP_3419: PP_3419 - Sigma-54 dependent transcriptional regulator/response regulator, at 3,869,491 to 3,870,918
_3419
PP_3420: PP_3420 - Sensor histidine kinase, at 3,870,887 to 3,872,380
_3420
PP_3421: PP_3421 - Sensor histidine kinase, at 3,872,688 to 3,874,139
_3421
Position (kb)
3870
3871
3872
3873 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3870.184 kb on - strand, within PP_3419 at 3870.601 kb on + strand, within PP_3419 at 3870.839 kb on + strand at 3870.840 kb on - strand at 3870.857 kb on + strand at 3870.858 kb on - strand at 3870.938 kb on - strand at 3870.946 kb on + strand at 3870.946 kb on + strand at 3870.947 kb on - strand at 3870.947 kb on - strand at 3870.947 kb on - strand at 3870.947 kb on - strand at 3871.508 kb on + strand, within PP_3420 at 3871.508 kb on + strand, within PP_3420 at 3871.509 kb on - strand, within PP_3420 at 3871.509 kb on - strand, within PP_3420 at 3871.509 kb on - strand, within PP_3420 at 3871.572 kb on + strand, within PP_3420 at 3871.573 kb on - strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.653 kb on + strand, within PP_3420 at 3871.653 kb on + strand, within PP_3420 at 3871.654 kb on - strand, within PP_3420 at 3871.654 kb on - strand, within PP_3420 at 3871.698 kb on + strand, within PP_3420 at 3871.699 kb on - strand, within PP_3420 at 3871.761 kb on - strand, within PP_3420 at 3871.761 kb on - strand, within PP_3420 at 3871.794 kb on + strand, within PP_3420 at 3871.812 kb on + strand, within PP_3420 at 3871.813 kb on - strand, within PP_3420 at 3871.813 kb on - strand, within PP_3420 at 3871.867 kb on - strand, within PP_3420 at 3871.867 kb on - strand, within PP_3420 at 3871.984 kb on - strand, within PP_3420 at 3871.984 kb on - strand, within PP_3420 at 3872.261 kb on - strand at 3872.403 kb on + strand at 3872.447 kb on - strand at 3872.585 kb on - strand at 3872.596 kb on + strand at 3872.843 kb on + strand, within PP_3421 at 3872.916 kb on + strand, within PP_3421 at 3872.916 kb on + strand, within PP_3421 at 3872.917 kb on - strand, within PP_3421 at 3872.978 kb on + strand, within PP_3421 at 3872.979 kb on - strand, within PP_3421 at 3872.985 kb on + strand, within PP_3421 at 3872.985 kb on + strand, within PP_3421 at 3873.031 kb on - strand, within PP_3421 at 3873.052 kb on - strand, within PP_3421 at 3873.079 kb on + strand, within PP_3421 at 3873.079 kb on + strand, within PP_3421 at 3873.080 kb on - strand, within PP_3421 at 3873.080 kb on - strand, within PP_3421 at 3873.080 kb on - strand, within PP_3421 at 3873.126 kb on + strand, within PP_3421 at 3873.203 kb on + strand, within PP_3421 at 3873.203 kb on + strand, within PP_3421 at 3873.293 kb on + strand, within PP_3421
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 3,870,184 - PP_3419 0.49 -0.3 3,870,601 + PP_3419 0.78 +0.8 3,870,839 + -0.2 3,870,840 - -0.7 3,870,857 + -0.1 3,870,858 - -0.2 3,870,938 - -1.2 3,870,946 + -1.1 3,870,946 + -0.5 3,870,947 - -0.0 3,870,947 - +0.5 3,870,947 - -1.3 3,870,947 - -1.8 3,871,508 + PP_3420 0.42 -0.6 3,871,508 + PP_3420 0.42 -0.4 3,871,509 - PP_3420 0.42 +0.4 3,871,509 - PP_3420 0.42 -0.4 3,871,509 - PP_3420 0.42 -0.2 3,871,572 + PP_3420 0.46 +1.0 3,871,573 - PP_3420 0.46 -0.6 3,871,615 + PP_3420 0.49 -0.9 3,871,615 + PP_3420 0.49 +0.2 3,871,615 + PP_3420 0.49 -0.8 3,871,615 + PP_3420 0.49 +0.8 3,871,615 + PP_3420 0.49 -0.6 3,871,615 + PP_3420 0.49 +0.0 3,871,615 + PP_3420 0.49 -0.2 3,871,615 + PP_3420 0.49 -0.5 3,871,615 + PP_3420 0.49 +2.8 3,871,616 - PP_3420 0.49 +0.8 3,871,616 - PP_3420 0.49 +0.8 3,871,616 - PP_3420 0.49 -0.8 3,871,616 - PP_3420 0.49 -2.2 3,871,616 - PP_3420 0.49 -0.5 3,871,653 + PP_3420 0.51 -0.6 3,871,653 + PP_3420 0.51 -2.2 3,871,654 - PP_3420 0.51 +0.6 3,871,654 - PP_3420 0.51 -0.7 3,871,698 + PP_3420 0.54 -0.5 3,871,699 - PP_3420 0.54 -1.2 3,871,761 - PP_3420 0.59 -0.1 3,871,761 - PP_3420 0.59 -0.7 3,871,794 + PP_3420 0.61 -0.1 3,871,812 + PP_3420 0.62 -1.0 3,871,813 - PP_3420 0.62 -0.2 3,871,813 - PP_3420 0.62 -1.2 3,871,867 - PP_3420 0.66 +0.2 3,871,867 - PP_3420 0.66 +0.2 3,871,984 - PP_3420 0.73 -0.3 3,871,984 - PP_3420 0.73 +1.3 3,872,261 - +0.6 3,872,403 + +1.5 3,872,447 - +0.5 3,872,585 - -1.8 3,872,596 + -0.1 3,872,843 + PP_3421 0.11 +0.3 3,872,916 + PP_3421 0.16 -0.0 3,872,916 + PP_3421 0.16 +0.1 3,872,917 - PP_3421 0.16 -0.1 3,872,978 + PP_3421 0.20 +0.3 3,872,979 - PP_3421 0.20 -0.4 3,872,985 + PP_3421 0.20 -0.8 3,872,985 + PP_3421 0.20 +0.9 3,873,031 - PP_3421 0.24 -1.4 3,873,052 - PP_3421 0.25 +0.8 3,873,079 + PP_3421 0.27 +0.2 3,873,079 + PP_3421 0.27 +1.0 3,873,080 - PP_3421 0.27 +1.5 3,873,080 - PP_3421 0.27 +0.3 3,873,080 - PP_3421 0.27 +0.2 3,873,126 + PP_3421 0.30 -1.1 3,873,203 + PP_3421 0.35 +0.6 3,873,203 + PP_3421 0.35 +1.8 3,873,293 + PP_3421 0.42 +1.2
Or see this region's nucleotide sequence