Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5508 and PP_5509 are separated by 3 nucleotides PP_5509 and PP_5510 are separated by 3 nucleotides PP_5510 and PP_2272 are separated by 240 nucleotides PP_2272 and PP_5511 overlap by 11 nucleotides PP_5511 and PP_2273 are separated by 140 nucleotides
PP_5508: PP_5508 - conserved protein of unknown function, at 2,593,272 to 2,593,499
_5508
PP_5509: PP_5509 - conserved exported protein of unknown function, at 2,593,503 to 2,593,913
_5509
PP_5510: PP_5510 - protein of unknown function, at 2,593,917 to 2,594,111
_5510
PP_2272: PP_2272 - conserved protein of unknown function, at 2,594,352 to 2,594,957
_2272
PP_5511: PP_5511 - protein of unknown function, at 2,594,947 to 2,595,141
_5511
PP_2273: PP_2273 - DNA polymerase I from phage origin, at 2,595,282 to 2,597,204
_2273
Position (kb)
2594
2595 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2593.434 kb on - strand, within PP_5508 at 2593.569 kb on + strand, within PP_5509 at 2593.570 kb on - strand, within PP_5509 at 2593.757 kb on + strand, within PP_5509 at 2593.824 kb on + strand, within PP_5509 at 2593.825 kb on - strand, within PP_5509 at 2593.825 kb on - strand, within PP_5509 at 2593.845 kb on + strand, within PP_5509 at 2593.846 kb on - strand, within PP_5509 at 2593.866 kb on + strand, within PP_5509 at 2593.866 kb on + strand, within PP_5509 at 2593.866 kb on + strand, within PP_5509 at 2593.866 kb on + strand, within PP_5509 at 2593.867 kb on - strand, within PP_5509 at 2593.867 kb on - strand, within PP_5509 at 2593.872 kb on + strand, within PP_5509 at 2593.873 kb on - strand at 2593.873 kb on - strand at 2593.908 kb on - strand at 2593.911 kb on + strand at 2593.911 kb on + strand at 2593.911 kb on + strand at 2593.912 kb on - strand at 2593.912 kb on - strand at 2593.912 kb on - strand at 2594.076 kb on + strand, within PP_5510 at 2594.109 kb on + strand at 2594.109 kb on + strand at 2594.109 kb on + strand at 2594.110 kb on - strand at 2594.110 kb on - strand at 2594.187 kb on + strand at 2594.188 kb on - strand at 2594.188 kb on - strand at 2594.226 kb on + strand at 2594.226 kb on + strand at 2594.334 kb on + strand at 2594.335 kb on - strand at 2594.363 kb on + strand at 2594.363 kb on + strand at 2594.363 kb on + strand at 2594.364 kb on - strand at 2594.364 kb on - strand at 2594.364 kb on - strand at 2594.366 kb on + strand at 2594.367 kb on - strand at 2594.367 kb on - strand at 2594.367 kb on - strand at 2594.632 kb on - strand, within PP_2272 at 2594.645 kb on + strand, within PP_2272 at 2594.677 kb on + strand, within PP_2272 at 2594.677 kb on + strand, within PP_2272 at 2594.678 kb on - strand, within PP_2272 at 2594.769 kb on + strand, within PP_2272 at 2594.769 kb on + strand, within PP_2272 at 2594.769 kb on + strand, within PP_2272 at 2594.769 kb on + strand, within PP_2272 at 2594.769 kb on + strand, within PP_2272 at 2594.770 kb on - strand, within PP_2272 at 2594.770 kb on - strand, within PP_2272 at 2594.779 kb on - strand at 2594.914 kb on - strand at 2594.914 kb on - strand at 2594.914 kb on - strand at 2595.124 kb on - strand at 2595.254 kb on + strand at 2595.352 kb on - strand at 2595.378 kb on + strand at 2595.561 kb on + strand, within PP_2273 at 2595.562 kb on - strand, within PP_2273 at 2595.645 kb on + strand, within PP_2273 at 2595.705 kb on + strand, within PP_2273 at 2595.706 kb on - strand, within PP_2273 at 2595.706 kb on - strand, within PP_2273
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 2,593,434 - PP_5508 0.71 +0.5 2,593,569 + PP_5509 0.16 -0.7 2,593,570 - PP_5509 0.16 -0.8 2,593,757 + PP_5509 0.62 +0.5 2,593,824 + PP_5509 0.78 -0.2 2,593,825 - PP_5509 0.78 +0.2 2,593,825 - PP_5509 0.78 -1.7 2,593,845 + PP_5509 0.83 +0.5 2,593,846 - PP_5509 0.83 +0.6 2,593,866 + PP_5509 0.88 -1.5 2,593,866 + PP_5509 0.88 +0.2 2,593,866 + PP_5509 0.88 +0.5 2,593,866 + PP_5509 0.88 +0.5 2,593,867 - PP_5509 0.89 +0.8 2,593,867 - PP_5509 0.89 +0.2 2,593,872 + PP_5509 0.90 +1.0 2,593,873 - +0.5 2,593,873 - +1.2 2,593,908 - -0.2 2,593,911 + -2.0 2,593,911 + +1.5 2,593,911 + -0.6 2,593,912 - +0.7 2,593,912 - +0.4 2,593,912 - -0.2 2,594,076 + PP_5510 0.82 +1.0 2,594,109 + -0.8 2,594,109 + -0.5 2,594,109 + -0.0 2,594,110 - +2.0 2,594,110 - +0.2 2,594,187 + +0.2 2,594,188 - -0.4 2,594,188 - +0.9 2,594,226 + +0.5 2,594,226 + +0.7 2,594,334 + -0.4 2,594,335 - +0.6 2,594,363 + -0.3 2,594,363 + -1.4 2,594,363 + +0.4 2,594,364 - -0.7 2,594,364 - -2.9 2,594,364 - -0.5 2,594,366 + -0.1 2,594,367 - -0.9 2,594,367 - -0.7 2,594,367 - +0.3 2,594,632 - PP_2272 0.46 -0.7 2,594,645 + PP_2272 0.48 -0.9 2,594,677 + PP_2272 0.54 -1.4 2,594,677 + PP_2272 0.54 -2.0 2,594,678 - PP_2272 0.54 -0.4 2,594,769 + PP_2272 0.69 +1.6 2,594,769 + PP_2272 0.69 -1.3 2,594,769 + PP_2272 0.69 -0.2 2,594,769 + PP_2272 0.69 -0.1 2,594,769 + PP_2272 0.69 -0.8 2,594,770 - PP_2272 0.69 -0.6 2,594,770 - PP_2272 0.69 -0.2 2,594,779 - +0.0 2,594,914 - -0.5 2,594,914 - +0.7 2,594,914 - -1.0 2,595,124 - -0.4 2,595,254 + -0.4 2,595,352 - +0.2 2,595,378 + -1.5 2,595,561 + PP_2273 0.15 -0.3 2,595,562 - PP_2273 0.15 -0.1 2,595,645 + PP_2273 0.19 +0.1 2,595,705 + PP_2273 0.22 -1.4 2,595,706 - PP_2273 0.22 +1.0 2,595,706 - PP_2273 0.22 -0.1
Or see this region's nucleotide sequence