Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1663 and PP_1664 are separated by 8 nucleotides PP_1664 and PP_1665 overlap by 1 nucleotides PP_1665 and PP_1666 are separated by 320 nucleotides
PP_1663: PP_1663 - conserved exported protein of unknown function, at 1,859,564 to 1,860,274
_1663
PP_1664: PP_1664 - phosphoribosylglycinamide formyltransferase 1, at 1,860,283 to 1,860,936
_1664
PP_1665: PP_1665 - Phosphoribosylformylglycinamidine cyclo-ligase, at 1,860,936 to 1,861,994
_1665
PP_1666: PP_1666 - conserved exported protein of unknown function, at 1,862,315 to 1,863,382
_1666
Position (kb)
1860
1861
1862 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1860.024 kb on + strand, within PP_1663 at 1860.024 kb on + strand, within PP_1663 at 1860.025 kb on - strand, within PP_1663 at 1860.110 kb on - strand, within PP_1663 at 1860.110 kb on - strand, within PP_1663 at 1860.110 kb on - strand, within PP_1663 at 1860.193 kb on - strand, within PP_1663 at 1860.274 kb on + strand at 1860.285 kb on - strand at 1860.331 kb on + strand at 1860.331 kb on + strand at 1860.332 kb on - strand at 1860.393 kb on - strand, within PP_1664 at 1860.416 kb on - strand, within PP_1664 at 1860.515 kb on - strand, within PP_1664 at 1860.515 kb on - strand, within PP_1664 at 1860.515 kb on - strand, within PP_1664 at 1860.564 kb on + strand, within PP_1664 at 1860.564 kb on + strand, within PP_1664 at 1860.565 kb on - strand, within PP_1664 at 1860.578 kb on + strand, within PP_1664 at 1860.578 kb on + strand, within PP_1664 at 1860.579 kb on - strand, within PP_1664 at 1860.579 kb on - strand, within PP_1664 at 1860.579 kb on - strand, within PP_1664 at 1860.907 kb on + strand at 1860.907 kb on + strand at 1860.913 kb on + strand at 1860.913 kb on + strand at 1860.913 kb on + strand at 1860.913 kb on + strand at 1860.913 kb on + strand at 1860.913 kb on + strand at 1860.913 kb on + strand at 1860.914 kb on - strand at 1860.914 kb on - strand at 1860.914 kb on - strand at 1862.009 kb on - strand at 1862.155 kb on + strand at 1862.156 kb on - strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.201 kb on - strand at 1862.201 kb on - strand at 1862.201 kb on - strand at 1862.201 kb on - strand at 1862.201 kb on - strand at 1862.202 kb on + strand at 1862.202 kb on + strand at 1862.203 kb on - strand at 1862.203 kb on - strand at 1862.281 kb on + strand at 1862.330 kb on - strand at 1862.397 kb on - strand at 1862.397 kb on - strand at 1862.397 kb on - strand at 1862.467 kb on + strand, within PP_1666 at 1862.468 kb on - strand, within PP_1666 at 1862.497 kb on + strand, within PP_1666 at 1862.497 kb on + strand, within PP_1666 at 1862.497 kb on + strand, within PP_1666 at 1862.497 kb on + strand, within PP_1666 at 1862.614 kb on + strand, within PP_1666 at 1862.615 kb on - strand, within PP_1666 at 1862.883 kb on - strand, within PP_1666 at 1862.907 kb on + strand, within PP_1666 at 1862.908 kb on - strand, within PP_1666 at 1862.908 kb on - strand, within PP_1666 at 1862.908 kb on - strand, within PP_1666
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 1,860,024 + PP_1663 0.65 -0.1 1,860,024 + PP_1663 0.65 +0.8 1,860,025 - PP_1663 0.65 -0.3 1,860,110 - PP_1663 0.77 -0.2 1,860,110 - PP_1663 0.77 -1.1 1,860,110 - PP_1663 0.77 -0.4 1,860,193 - PP_1663 0.88 -0.9 1,860,274 + -0.8 1,860,285 - +1.9 1,860,331 + -1.6 1,860,331 + -2.0 1,860,332 - +0.5 1,860,393 - PP_1664 0.17 -0.1 1,860,416 - PP_1664 0.20 -0.4 1,860,515 - PP_1664 0.35 -4.6 1,860,515 - PP_1664 0.35 -4.7 1,860,515 - PP_1664 0.35 -4.3 1,860,564 + PP_1664 0.43 +0.3 1,860,564 + PP_1664 0.43 -4.6 1,860,565 - PP_1664 0.43 -1.8 1,860,578 + PP_1664 0.45 -0.7 1,860,578 + PP_1664 0.45 -2.0 1,860,579 - PP_1664 0.45 +0.9 1,860,579 - PP_1664 0.45 +1.9 1,860,579 - PP_1664 0.45 -1.1 1,860,907 + -1.7 1,860,907 + -2.0 1,860,913 + -1.8 1,860,913 + -2.0 1,860,913 + +0.9 1,860,913 + -0.9 1,860,913 + -1.8 1,860,913 + -2.3 1,860,913 + -2.5 1,860,914 - -1.4 1,860,914 - +0.3 1,860,914 - -1.8 1,862,009 - -1.1 1,862,155 + -0.7 1,862,156 - -2.0 1,862,200 + -0.1 1,862,200 + -1.1 1,862,200 + +0.8 1,862,200 + -0.1 1,862,200 + +2.1 1,862,200 + -0.7 1,862,200 + -0.1 1,862,201 - +0.9 1,862,201 - +0.1 1,862,201 - +0.8 1,862,201 - +0.6 1,862,201 - -0.5 1,862,202 + +1.9 1,862,202 + -0.4 1,862,203 - +0.6 1,862,203 - -0.5 1,862,281 + -0.6 1,862,330 - -0.9 1,862,397 - -0.8 1,862,397 - +2.5 1,862,397 - +1.0 1,862,467 + PP_1666 0.14 -0.5 1,862,468 - PP_1666 0.14 +1.3 1,862,497 + PP_1666 0.17 -1.7 1,862,497 + PP_1666 0.17 +0.2 1,862,497 + PP_1666 0.17 -0.2 1,862,497 + PP_1666 0.17 +0.5 1,862,614 + PP_1666 0.28 -1.6 1,862,615 - PP_1666 0.28 -0.9 1,862,883 - PP_1666 0.53 -0.3 1,862,907 + PP_1666 0.55 -1.5 1,862,908 - PP_1666 0.56 +1.9 1,862,908 - PP_1666 0.56 +1.6 1,862,908 - PP_1666 0.56 -0.7
Or see this region's nucleotide sequence