Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1646 and PP_1647 are separated by 35 nucleotides PP_1647 and PP_1648 overlap by 31 nucleotides PP_1648 and PP_1649 are separated by 31 nucleotides PP_1649 and PP_1650 are separated by 37 nucleotides
PP_1646: PP_1646 - putative Thioredoxin, at 1,839,604 to 1,840,068
_1646
PP_1647: PP_1647 - conserved protein of unknown function, at 1,840,104 to 1,840,535
_1647
PP_1648: PP_1648 - conserved protein of unknown function, at 1,840,505 to 1,840,942
_1648
PP_1649: PP_1649 - D-lactate dehydrogenase, at 1,840,974 to 1,842,002
_1649
PP_1650: PP_1650 - Sensor protein GacS, at 1,842,040 to 1,844,793
_1650
Position (kb)
1840
1841
1842
1843 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1840.192 kb on - strand, within PP_1647 at 1840.340 kb on - strand, within PP_1647 at 1840.381 kb on + strand, within PP_1647 at 1840.382 kb on - strand, within PP_1647 at 1840.420 kb on + strand, within PP_1647 at 1840.421 kb on - strand, within PP_1647 at 1840.421 kb on - strand, within PP_1647 at 1840.921 kb on - strand at 1840.969 kb on + strand at 1840.970 kb on - strand at 1840.976 kb on - strand at 1841.186 kb on - strand, within PP_1649 at 1841.382 kb on + strand, within PP_1649 at 1841.447 kb on - strand, within PP_1649 at 1841.593 kb on + strand, within PP_1649 at 1841.728 kb on + strand, within PP_1649 at 1841.933 kb on - strand at 1842.163 kb on + strand at 1842.164 kb on - strand at 1842.183 kb on - strand at 1842.183 kb on - strand at 1842.208 kb on + strand at 1842.208 kb on + strand at 1842.208 kb on + strand at 1842.209 kb on - strand at 1842.209 kb on - strand at 1842.256 kb on + strand at 1842.256 kb on + strand at 1842.362 kb on - strand, within PP_1650 at 1842.486 kb on - strand, within PP_1650 at 1842.682 kb on + strand, within PP_1650 at 1842.683 kb on - strand, within PP_1650 at 1842.712 kb on + strand, within PP_1650 at 1842.745 kb on + strand, within PP_1650 at 1842.923 kb on + strand, within PP_1650 at 1842.923 kb on + strand, within PP_1650 at 1842.923 kb on + strand, within PP_1650 at 1842.933 kb on - strand, within PP_1650 at 1842.965 kb on - strand, within PP_1650 at 1842.965 kb on - strand, within PP_1650 at 1843.001 kb on + strand, within PP_1650 at 1843.001 kb on + strand, within PP_1650 at 1843.002 kb on - strand, within PP_1650
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 1,840,192 - PP_1647 0.20 -2.1 1,840,340 - PP_1647 0.55 +2.0 1,840,381 + PP_1647 0.64 -0.4 1,840,382 - PP_1647 0.64 +0.6 1,840,420 + PP_1647 0.73 -2.4 1,840,421 - PP_1647 0.73 +0.7 1,840,421 - PP_1647 0.73 -0.7 1,840,921 - +1.9 1,840,969 + -0.2 1,840,970 - -0.6 1,840,976 - -1.9 1,841,186 - PP_1649 0.21 -2.2 1,841,382 + PP_1649 0.40 -1.8 1,841,447 - PP_1649 0.46 -1.2 1,841,593 + PP_1649 0.60 +0.4 1,841,728 + PP_1649 0.73 -2.0 1,841,933 - -0.6 1,842,163 + -2.5 1,842,164 - -2.7 1,842,183 - -2.1 1,842,183 - -2.0 1,842,208 + -2.1 1,842,208 + -1.8 1,842,208 + -2.5 1,842,209 - -2.4 1,842,209 - -2.1 1,842,256 + -3.9 1,842,256 + -2.0 1,842,362 - PP_1650 0.12 -3.5 1,842,486 - PP_1650 0.16 -3.1 1,842,682 + PP_1650 0.23 -2.0 1,842,683 - PP_1650 0.23 -2.1 1,842,712 + PP_1650 0.24 -3.4 1,842,745 + PP_1650 0.26 -3.4 1,842,923 + PP_1650 0.32 -1.8 1,842,923 + PP_1650 0.32 -2.0 1,842,923 + PP_1650 0.32 -2.1 1,842,933 - PP_1650 0.32 -2.3 1,842,965 - PP_1650 0.34 -2.9 1,842,965 - PP_1650 0.34 -3.1 1,843,001 + PP_1650 0.35 -2.2 1,843,001 + PP_1650 0.35 -1.9 1,843,002 - PP_1650 0.35 -1.9
Or see this region's nucleotide sequence