Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1100 and PP_1101 are separated by 61 nucleotides PP_1101 and PP_1102 are separated by 6 nucleotides PP_1102 and PP_1103 overlap by 4 nucleotides
PP_1100: PP_1100 - Deoxycytidine triphosphate deaminase, at 1,257,458 to 1,258,024
_1100
PP_1101: PP_1101 - conserved protein of unknown function, at 1,258,086 to 1,258,346
_1101
PP_1102: PP_1102 - conserved protein of unknown function, at 1,258,353 to 1,259,003
_1102
PP_1103: PP_1103 - ATP-dependent helicase, DEAD box family, at 1,259,000 to 1,261,450
_1103
Position (kb)
1258
1259
1260 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1257.388 kb on - strand at 1257.411 kb on + strand at 1257.411 kb on + strand at 1257.584 kb on + strand at 1257.591 kb on - strand, within PP_1100 at 1257.591 kb on - strand at 1257.747 kb on - strand, within PP_1100 at 1257.797 kb on + strand at 1257.966 kb on - strand at 1257.987 kb on - strand at 1258.072 kb on + strand at 1258.355 kb on - strand at 1258.388 kb on - strand at 1258.388 kb on - strand at 1258.449 kb on + strand, within PP_1102 at 1258.449 kb on + strand, within PP_1102 at 1258.533 kb on + strand, within PP_1102 at 1258.533 kb on + strand, within PP_1102 at 1258.533 kb on + strand, within PP_1102 at 1258.533 kb on + strand, within PP_1102 at 1258.534 kb on - strand, within PP_1102 at 1258.534 kb on - strand, within PP_1102 at 1258.534 kb on - strand, within PP_1102 at 1258.534 kb on - strand, within PP_1102 at 1258.939 kb on + strand at 1258.939 kb on + strand at 1258.996 kb on + strand at 1258.997 kb on - strand at 1259.210 kb on + strand at 1259.211 kb on - strand at 1259.211 kb on - strand at 1259.271 kb on + strand, within PP_1103 at 1259.271 kb on + strand, within PP_1103 at 1259.271 kb on + strand, within PP_1103 at 1259.271 kb on + strand, within PP_1103 at 1259.271 kb on + strand, within PP_1103 at 1259.272 kb on - strand, within PP_1103 at 1259.272 kb on - strand, within PP_1103 at 1259.272 kb on - strand, within PP_1103 at 1259.272 kb on - strand, within PP_1103 at 1259.277 kb on - strand, within PP_1103 at 1259.277 kb on - strand, within PP_1103 at 1259.277 kb on - strand, within PP_1103 at 1259.598 kb on + strand, within PP_1103 at 1259.702 kb on + strand, within PP_1103 at 1259.850 kb on + strand, within PP_1103 at 1259.850 kb on + strand, within PP_1103 at 1259.851 kb on - strand, within PP_1103 at 1259.851 kb on - strand, within PP_1103 at 1259.882 kb on + strand, within PP_1103 at 1259.882 kb on + strand, within PP_1103 at 1259.883 kb on - strand, within PP_1103 at 1259.883 kb on - strand, within PP_1103 at 1259.885 kb on + strand, within PP_1103 at 1259.985 kb on + strand, within PP_1103 at 1259.986 kb on - strand, within PP_1103 at 1259.986 kb on - strand, within PP_1103 at 1259.986 kb on - strand, within PP_1103
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 1,257,388 - +2.2 1,257,411 + -1.0 1,257,411 + -0.6 1,257,584 + +2.2 1,257,591 - PP_1100 0.23 +2.2 1,257,591 - +2.2 1,257,747 - PP_1100 0.51 +1.2 1,257,797 + +1.2 1,257,966 - +2.2 1,257,987 - +2.2 1,258,072 + -0.4 1,258,355 - -0.2 1,258,388 - -0.8 1,258,388 - +0.5 1,258,449 + PP_1102 0.15 -0.1 1,258,449 + PP_1102 0.15 -0.4 1,258,533 + PP_1102 0.28 -0.5 1,258,533 + PP_1102 0.28 -0.8 1,258,533 + PP_1102 0.28 -0.8 1,258,533 + PP_1102 0.28 -0.2 1,258,534 - PP_1102 0.28 -1.3 1,258,534 - PP_1102 0.28 -0.2 1,258,534 - PP_1102 0.28 -1.4 1,258,534 - PP_1102 0.28 -2.9 1,258,939 + +0.0 1,258,939 + -2.0 1,258,996 + -0.2 1,258,997 - +0.6 1,259,210 + -1.4 1,259,211 - +1.2 1,259,211 - +0.6 1,259,271 + PP_1103 0.11 -0.5 1,259,271 + PP_1103 0.11 -0.1 1,259,271 + PP_1103 0.11 -0.2 1,259,271 + PP_1103 0.11 +0.8 1,259,271 + PP_1103 0.11 -1.2 1,259,272 - PP_1103 0.11 -1.1 1,259,272 - PP_1103 0.11 +0.2 1,259,272 - PP_1103 0.11 +0.2 1,259,272 - PP_1103 0.11 -0.0 1,259,277 - PP_1103 0.11 +0.8 1,259,277 - PP_1103 0.11 +0.5 1,259,277 - PP_1103 0.11 -0.2 1,259,598 + PP_1103 0.24 +0.9 1,259,702 + PP_1103 0.29 -0.4 1,259,850 + PP_1103 0.35 -0.8 1,259,850 + PP_1103 0.35 +0.9 1,259,851 - PP_1103 0.35 +0.3 1,259,851 - PP_1103 0.35 -1.9 1,259,882 + PP_1103 0.36 -0.3 1,259,882 + PP_1103 0.36 -0.2 1,259,883 - PP_1103 0.36 -1.1 1,259,883 - PP_1103 0.36 -0.8 1,259,885 + PP_1103 0.36 +0.3 1,259,985 + PP_1103 0.40 +1.0 1,259,986 - PP_1103 0.40 -0.8 1,259,986 - PP_1103 0.40 +0.2 1,259,986 - PP_1103 0.40 +0.2
Or see this region's nucleotide sequence