Strain Fitness in Pseudomonas putida KT2440 around PP_0715

Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0714 and PP_0715 are separated by 3 nucleotidesPP_0715 and PP_0716 overlap by 4 nucleotides PP_0714: PP_0714 - Transporter, PerM family, at 829,626 to 830,723 _0714 PP_0715: PP_0715 - Outer membrane efflux protein, at 830,727 to 832,241 _0715 PP_0716: PP_0716 - HlyD family secretion protein, at 832,238 to 833,335 _0716 Position (kb) 830 831 832 833Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 829.786 kb on - strand, within PP_0714at 829.883 kb on + strand, within PP_0714at 829.883 kb on + strand, within PP_0714at 829.883 kb on + strand, within PP_0714at 829.884 kb on - strand, within PP_0714at 829.884 kb on - strand, within PP_0714at 829.887 kb on + strand, within PP_0714at 829.887 kb on + strand, within PP_0714at 829.887 kb on + strand, within PP_0714at 829.888 kb on - strand, within PP_0714at 829.888 kb on - strand, within PP_0714at 829.888 kb on - strand, within PP_0714at 830.054 kb on - strand, within PP_0714at 830.054 kb on - strand, within PP_0714at 830.347 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.445 kb on + strand, within PP_0714at 830.446 kb on - strand, within PP_0714at 830.446 kb on - strand, within PP_0714at 830.446 kb on - strand, within PP_0714at 830.496 kb on + strand, within PP_0714at 830.496 kb on + strand, within PP_0714at 830.497 kb on - strand, within PP_0714at 830.497 kb on - strand, within PP_0714at 830.564 kb on - strand, within PP_0714at 830.564 kb on - strand, within PP_0714at 830.578 kb on + strand, within PP_0714at 830.593 kb on + strand, within PP_0714at 830.593 kb on + strand, within PP_0714at 830.593 kb on + strand, within PP_0714at 830.593 kb on + strand, within PP_0714at 830.593 kb on + strand, within PP_0714at 830.594 kb on - strand, within PP_0714at 830.594 kb on - strand, within PP_0714at 830.594 kb on - strand, within PP_0714at 830.594 kb on - strand, within PP_0714at 830.631 kb on - strandat 830.641 kb on + strandat 830.658 kb on + strandat 830.658 kb on + strandat 830.658 kb on + strandat 830.658 kb on + strandat 830.658 kb on + strandat 830.658 kb on + strandat 830.658 kb on + strandat 830.658 kb on + strandat 830.659 kb on - strandat 830.743 kb on - strandat 830.743 kb on - strandat 831.060 kb on + strand, within PP_0715at 831.083 kb on + strand, within PP_0715at 831.219 kb on + strand, within PP_0715at 831.219 kb on + strand, within PP_0715at 831.219 kb on + strand, within PP_0715at 831.219 kb on + strand, within PP_0715at 831.220 kb on - strand, within PP_0715at 831.220 kb on - strand, within PP_0715at 831.264 kb on + strand, within PP_0715at 831.264 kb on + strand, within PP_0715at 831.265 kb on - strand, within PP_0715at 831.265 kb on - strand, within PP_0715at 831.408 kb on + strand, within PP_0715at 831.408 kb on + strand, within PP_0715at 831.409 kb on - strand, within PP_0715at 831.409 kb on - strand, within PP_0715at 831.469 kb on + strand, within PP_0715at 831.469 kb on + strand, within PP_0715at 831.469 kb on + strand, within PP_0715at 831.469 kb on + strand, within PP_0715at 831.469 kb on + strand, within PP_0715at 831.469 kb on + strand, within PP_0715at 831.470 kb on - strand, within PP_0715at 831.470 kb on - strand, within PP_0715at 831.470 kb on - strand, within PP_0715at 831.569 kb on - strand, within PP_0715at 831.569 kb on - strand, within PP_0715at 831.569 kb on - strand, within PP_0715at 831.569 kb on - strand, within PP_0715at 831.569 kb on - strand, within PP_0715at 831.569 kb on - strand, within PP_0715at 831.780 kb on + strand, within PP_0715at 831.802 kb on + strand, within PP_0715at 831.802 kb on + strand, within PP_0715at 831.802 kb on + strand, within PP_0715at 831.802 kb on + strand, within PP_0715at 831.802 kb on + strand, within PP_0715at 831.803 kb on - strand, within PP_0715at 831.824 kb on - strand, within PP_0715at 831.843 kb on + strand, within PP_0715at 832.054 kb on - strand, within PP_0715at 832.054 kb on - strand, within PP_0715at 832.098 kb on + strandat 832.098 kb on + strandat 832.098 kb on + strandat 832.099 kb on - strandat 832.099 kb on - strandat 832.099 kb on - strandat 832.099 kb on - strandat 832.287 kb on + strandat 832.288 kb on - strandat 832.288 kb on - strandat 832.328 kb on + strandat 832.328 kb on + strandat 832.329 kb on - strandat 832.348 kb on + strand, within PP_0716at 832.348 kb on + strand, within PP_0716at 832.348 kb on + strand, within PP_0716at 832.349 kb on - strand, within PP_0716at 832.349 kb on - strand, within PP_0716at 832.355 kb on + strand, within PP_0716at 832.382 kb on + strand, within PP_0716at 832.382 kb on + strand, within PP_0716at 832.382 kb on + strand, within PP_0716at 832.382 kb on + strand, within PP_0716at 832.383 kb on - strand, within PP_0716at 832.383 kb on - strand, within PP_0716at 832.383 kb on - strand, within PP_0716at 832.567 kb on + strand, within PP_0716at 832.853 kb on - strand, within PP_0716at 832.913 kb on + strand, within PP_0716at 832.913 kb on + strand, within PP_0716at 832.913 kb on + strand, within PP_0716at 832.914 kb on - strand, within PP_0716at 832.928 kb on - strand, within PP_0716at 832.951 kb on + strand, within PP_0716at 832.969 kb on - strand, within PP_0716at 832.969 kb on - strand, within PP_0716at 833.068 kb on + strand, within PP_0716at 833.069 kb on - strand, within PP_0716at 833.069 kb on - strand, within PP_0716at 833.069 kb on - strand, within PP_0716at 833.069 kb on - strand, within PP_0716at 833.070 kb on + strand, within PP_0716at 833.071 kb on - strand, within PP_0716at 833.071 kb on - strand, within PP_0716at 833.071 kb on - strand, within PP_0716at 833.141 kb on - strand, within PP_0716at 833.183 kb on - strand, within PP_0716

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Per-strain Table

Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
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829,786 - PP_0714 0.15 +2.3
829,883 + PP_0714 0.23 +0.9
829,883 + PP_0714 0.23 -0.6
829,883 + PP_0714 0.23 -0.2
829,884 - PP_0714 0.23 -0.3
829,884 - PP_0714 0.23 +0.2
829,887 + PP_0714 0.24 -0.4
829,887 + PP_0714 0.24 -1.1
829,887 + PP_0714 0.24 -0.6
829,888 - PP_0714 0.24 -1.5
829,888 - PP_0714 0.24 +0.1
829,888 - PP_0714 0.24 -0.5
830,054 - PP_0714 0.39 -2.8
830,054 - PP_0714 0.39 -0.3
830,347 + PP_0714 0.66 -0.1
830,445 + PP_0714 0.75 +0.5
830,445 + PP_0714 0.75 -0.8
830,445 + PP_0714 0.75 -1.7
830,445 + PP_0714 0.75 -0.2
830,445 + PP_0714 0.75 +1.1
830,445 + PP_0714 0.75 -0.5
830,445 + PP_0714 0.75 +0.1
830,445 + PP_0714 0.75 +0.3
830,445 + PP_0714 0.75 +1.3
830,445 + PP_0714 0.75 +0.2
830,446 - PP_0714 0.75 -2.8
830,446 - PP_0714 0.75 -0.1
830,446 - PP_0714 0.75 +0.5
830,496 + PP_0714 0.79 +0.2
830,496 + PP_0714 0.79 +0.1
830,497 - PP_0714 0.79 -0.8
830,497 - PP_0714 0.79 +0.3
830,564 - PP_0714 0.85 +0.5
830,564 - PP_0714 0.85 +0.3
830,578 + PP_0714 0.87 -0.2
830,593 + PP_0714 0.88 -0.8
830,593 + PP_0714 0.88 +0.8
830,593 + PP_0714 0.88 +0.5
830,593 + PP_0714 0.88 -1.6
830,593 + PP_0714 0.88 -0.3
830,594 - PP_0714 0.88 -0.2
830,594 - PP_0714 0.88 +0.8
830,594 - PP_0714 0.88 +1.3
830,594 - PP_0714 0.88 +1.2
830,631 - -1.0
830,641 + +1.8
830,658 + -1.2
830,658 + +0.0
830,658 + +0.3
830,658 + +1.3
830,658 + -0.2
830,658 + -1.1
830,658 + +1.8
830,658 + -1.9
830,659 - -2.0
830,743 - +0.2
830,743 - +0.6
831,060 + PP_0715 0.22 +0.6
831,083 + PP_0715 0.23 +0.3
831,219 + PP_0715 0.32 +0.9
831,219 + PP_0715 0.32 +0.8
831,219 + PP_0715 0.32 -1.0
831,219 + PP_0715 0.32 +0.3
831,220 - PP_0715 0.33 -2.5
831,220 - PP_0715 0.33 -1.6
831,264 + PP_0715 0.35 -0.5
831,264 + PP_0715 0.35 -1.4
831,265 - PP_0715 0.36 -0.0
831,265 - PP_0715 0.36 +0.7
831,408 + PP_0715 0.45 -1.3
831,408 + PP_0715 0.45 +0.2
831,409 - PP_0715 0.45 -3.3
831,409 - PP_0715 0.45 +0.3
831,469 + PP_0715 0.49 -1.2
831,469 + PP_0715 0.49 -0.1
831,469 + PP_0715 0.49 +0.6
831,469 + PP_0715 0.49 -0.8
831,469 + PP_0715 0.49 -1.7
831,469 + PP_0715 0.49 -1.0
831,470 - PP_0715 0.49 -0.5
831,470 - PP_0715 0.49 -0.7
831,470 - PP_0715 0.49 -0.1
831,569 - PP_0715 0.56 +0.3
831,569 - PP_0715 0.56 -0.4
831,569 - PP_0715 0.56 +0.2
831,569 - PP_0715 0.56 +0.3
831,569 - PP_0715 0.56 +1.3
831,569 - PP_0715 0.56 -0.3
831,780 + PP_0715 0.70 +1.4
831,802 + PP_0715 0.71 -0.4
831,802 + PP_0715 0.71 -0.6
831,802 + PP_0715 0.71 -1.4
831,802 + PP_0715 0.71 -1.0
831,802 + PP_0715 0.71 +1.3
831,803 - PP_0715 0.71 -1.6
831,824 - PP_0715 0.72 -0.1
831,843 + PP_0715 0.74 +0.0
832,054 - PP_0715 0.88 -0.6
832,054 - PP_0715 0.88 -1.5
832,098 + +0.3
832,098 + -2.0
832,098 + +1.3
832,099 - -0.8
832,099 - -0.1
832,099 - -2.7
832,099 - -0.5
832,287 + -0.7
832,288 - +1.1
832,288 - +0.4
832,328 + -0.5
832,328 + +0.7
832,329 - -0.5
832,348 + PP_0716 0.10 -0.2
832,348 + PP_0716 0.10 +1.3
832,348 + PP_0716 0.10 -1.4
832,349 - PP_0716 0.10 +0.1
832,349 - PP_0716 0.10 +0.3
832,355 + PP_0716 0.11 -1.3
832,382 + PP_0716 0.13 -0.4
832,382 + PP_0716 0.13 +0.2
832,382 + PP_0716 0.13 -0.5
832,382 + PP_0716 0.13 -0.5
832,383 - PP_0716 0.13 +0.1
832,383 - PP_0716 0.13 +0.6
832,383 - PP_0716 0.13 +0.3
832,567 + PP_0716 0.30 -0.6
832,853 - PP_0716 0.56 -0.5
832,913 + PP_0716 0.61 -0.4
832,913 + PP_0716 0.61 -0.2
832,913 + PP_0716 0.61 +0.7
832,914 - PP_0716 0.62 +0.1
832,928 - PP_0716 0.63 -0.1
832,951 + PP_0716 0.65 +1.2
832,969 - PP_0716 0.67 -1.3
832,969 - PP_0716 0.67 +0.3
833,068 + PP_0716 0.76 -1.2
833,069 - PP_0716 0.76 -0.3
833,069 - PP_0716 0.76 -1.6
833,069 - PP_0716 0.76 +0.1
833,069 - PP_0716 0.76 +1.8
833,070 + PP_0716 0.76 +1.6
833,071 - PP_0716 0.76 -0.9
833,071 - PP_0716 0.76 -1.0
833,071 - PP_0716 0.76 +0.1
833,141 - PP_0716 0.82 +0.5
833,183 - PP_0716 0.86 +1.3

Or see this region's nucleotide sequence