Experiment: MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5106 and PP_5107 are separated by 64 nucleotides PP_5107 and PP_5108 are separated by 198 nucleotides PP_5108 and PP_5109 are separated by 111 nucleotides
PP_5106: PP_5106 - conserved inner membrane protein, at 5,826,652 to 5,827,023
_5106
PP_5107: PP_5107 - Monofunctional biosynthetic peptidoglycan transglycosylase, at 5,827,088 to 5,827,798
_5107
PP_5108: PP_5108 - RNA polymerase factor sigma-32, at 5,827,997 to 5,828,851
_5108
PP_5109: PP_5109 - membrane component of the ABC transporter FtsEX involved in cellular division, at 5,828,963 to 5,829,988
_5109
Position (kb) 5827
5828
5829 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5827.038 kb on + strand at 5827.049 kb on + strand at 5827.049 kb on + strand at 5827.049 kb on + strand at 5827.049 kb on + strand at 5827.050 kb on - strand at 5827.050 kb on - strand at 5827.050 kb on - strand at 5827.050 kb on - strand at 5827.050 kb on - strand at 5827.051 kb on + strand at 5827.051 kb on + strand at 5827.052 kb on - strand at 5827.052 kb on - strand at 5827.103 kb on - strand at 5827.215 kb on + strand, within PP_5107 at 5827.541 kb on + strand, within PP_5107 at 5827.541 kb on + strand, within PP_5107 at 5827.541 kb on + strand, within PP_5107 at 5827.542 kb on - strand, within PP_5107 at 5827.542 kb on - strand, within PP_5107 at 5827.542 kb on - strand, within PP_5107 at 5827.703 kb on + strand, within PP_5107 at 5827.703 kb on + strand, within PP_5107 at 5827.836 kb on - strand at 5827.901 kb on + strand at 5827.902 kb on - strand at 5828.869 kb on - strand at 5828.919 kb on - strand at 5828.919 kb on - strand at 5828.958 kb on - strand at 5829.017 kb on - strand at 5829.017 kb on - strand at 5829.019 kb on - strand at 5829.096 kb on + strand, within PP_5109 at 5829.096 kb on + strand, within PP_5109 at 5829.186 kb on + strand, within PP_5109 at 5829.186 kb on + strand, within PP_5109 at 5829.186 kb on + strand, within PP_5109 at 5829.186 kb on + strand, within PP_5109 at 5829.187 kb on - strand, within PP_5109 at 5829.187 kb on - strand, within PP_5109 at 5829.201 kb on + strand, within PP_5109 at 5829.201 kb on + strand, within PP_5109 at 5829.201 kb on + strand, within PP_5109 at 5829.222 kb on + strand, within PP_5109 at 5829.223 kb on - strand, within PP_5109 at 5829.266 kb on - strand, within PP_5109 at 5829.305 kb on + strand, within PP_5109 at 5829.306 kb on - strand, within PP_5109 at 5829.309 kb on - strand, within PP_5109 at 5829.591 kb on + strand, within PP_5109 at 5829.591 kb on + strand, within PP_5109 at 5829.592 kb on - strand, within PP_5109 at 5829.592 kb on - strand, within PP_5109 at 5829.675 kb on + strand, within PP_5109
Per-strain Table
Position Strand Gene LocusTag Fraction MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL remove 5,827,038 + -0.3 5,827,049 + -0.5 5,827,049 + -0.2 5,827,049 + -0.7 5,827,049 + +0.7 5,827,050 - +1.5 5,827,050 - -0.9 5,827,050 - -1.0 5,827,050 - +1.5 5,827,050 - +0.4 5,827,051 + -0.4 5,827,051 + -0.8 5,827,052 - -0.2 5,827,052 - +0.9 5,827,103 - -0.5 5,827,215 + PP_5107 0.18 +2.2 5,827,541 + PP_5107 0.64 +2.5 5,827,541 + PP_5107 0.64 -0.7 5,827,541 + PP_5107 0.64 -0.1 5,827,542 - PP_5107 0.64 -0.8 5,827,542 - PP_5107 0.64 -0.0 5,827,542 - PP_5107 0.64 -0.0 5,827,703 + PP_5107 0.86 -0.1 5,827,703 + PP_5107 0.86 +0.8 5,827,836 - -1.5 5,827,901 + +0.7 5,827,902 - +0.6 5,828,869 - -0.9 5,828,919 - +0.1 5,828,919 - -0.1 5,828,958 - +0.9 5,829,017 - -0.7 5,829,017 - +1.3 5,829,019 - +0.2 5,829,096 + PP_5109 0.13 +0.6 5,829,096 + PP_5109 0.13 -1.4 5,829,186 + PP_5109 0.22 -1.2 5,829,186 + PP_5109 0.22 -0.8 5,829,186 + PP_5109 0.22 -1.7 5,829,186 + PP_5109 0.22 +0.0 5,829,187 - PP_5109 0.22 -0.1 5,829,187 - PP_5109 0.22 +2.2 5,829,201 + PP_5109 0.23 +0.4 5,829,201 + PP_5109 0.23 +1.4 5,829,201 + PP_5109 0.23 -0.7 5,829,222 + PP_5109 0.25 +0.5 5,829,223 - PP_5109 0.25 +1.5 5,829,266 - PP_5109 0.30 -1.4 5,829,305 + PP_5109 0.33 -1.9 5,829,306 - PP_5109 0.33 -1.3 5,829,309 - PP_5109 0.34 +1.5 5,829,591 + PP_5109 0.61 -0.4 5,829,591 + PP_5109 0.61 +0.5 5,829,592 - PP_5109 0.61 +0.9 5,829,592 - PP_5109 0.61 -0.3 5,829,675 + PP_5109 0.69 -0.2
Or see this region's nucleotide sequence